Uniqueness of Sperm mtDNA as Compared to Somatic mtDNA in Ram

Bartoov, B.; Fisher, J.

doi:10.1111/j.1365-2605.1980.tb00147.x

Cited by 6 publications

(2 citation statements)

References 31 publications

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“…It has been shown that sperm mitochondria are more dense and contain a distinct polypeptide which is not found in other mitochondria, suggesting the occurrence of significant macromolecular changes in paternal mitochondria (Hecht & Bradley, 1981). However, on the basis of evidence that mitochondrial DNA of ram sperm has a different density, base composition and counter length from somatic mitochondrial DNA, Bartoov & Fisher (1980) suggested that the genome of sperm mitochondria alters during spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

Sperm head decondensation, pronuclear formation, cleavage and embryonic development following intracytoplasmic injection of mitochondria-damaged sperm in mammals

Ahmadi

1997

Zygote

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The objective of this study was to investigate the influence of sperm mitochondrial destruction on sperm head decondensation, male pronuclear formation, cleavage and embryonic development. In the study two models were used: heterologous (hamster ICSI assay: human sperm injected into a hamster oocyte) for evaluation of sperm head decondensation and pronuclear formation, and homologous (mouse model) for the study of fertilisation and development. Destruction of mitochondria of the sperm was achieved by exposure to cyanide, a respiratory poison. Rhodamine 123 was used to evaluate the functional integrity of mitochondria. Sperm head decondensation was found to be not statistically significantly affected by mitochondrial damage (p = 0.8), with 62.8% and 67.9% condensation in the experimental and control groups respectively. Male pronucleus formation was seen in 40.2% and 44.4% of the injected oocytes in the experimental and control groups respectively. In the mouse experiments 45.5% and 49.7% of the injected oocytes were fertilised in the mitochondria-damaged and live-intact sperm groups respectively (p = 0.53). Development to blastocyst was achieved in 53.5% and 59.4% of the experimental and control groups respectively; the difference was not significant (p = 0.71). Inner cell mass (ICM) cell number were 15.7 + 4.02 and 43.1+11.3 respectively in the mitochondriadamaged group; the equivalent numbers were 14.12 + 4.12 and 39.3 + 12.6 in the control group. However, the differences in ICM and total cell counts between these two groups were not significant. Of the blastocysts transferred to pseudopregnant mice, 51.3% (20/36) implanted and 33.4% (12/36) developed to live fetuses in the mitochondria-damaged group. These rates were 60.5% (23/38) and 39.5% (15/38) in the control group. In conclusion, this study shows that functional integrity of the sperm mitochondria is not necessary in the process of fertilisation and development when the sperm is deposited into the ooplasm. Fertilisation and development can be achieved by injection of sperm at the very early stage of necrosis in which only the mitochondria have been destroyed and the rest of the cell including the plasma membrane is still intact.

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Section: Discussionmentioning

confidence: 99%

Sperm head decondensation, pronuclear formation, cleavage and embryonic development following intracytoplasmic injection of mitochondria-damaged sperm in mammals

Ahmadi

1997

Zygote

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“…In these studies in rats, the type of mtDNA extracted from the various tissues was always the same type for a given rat. However, some species exhibit heterogeneity of mtDNA in various tissues from the same animal (Coote, Szabados, and Work, 1979;Bartoov and Fisher, 1980;Hauswirth et al, 1984).…”

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confidence: 99%

Mitochondrial DNA in somatic and germinal cells of male rats, and embryo transfer between donors and recipients of known mitochondrial DNA

Alcivar

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Isolation and Characterization of Mitochondria from Turkey Spermatozoa

MCLEAN,

KORN,

PEREZ

et al. 1993

Journal of Andrology

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A procedure was developed to rapidly isolate functional, intact mitochondria from turkey spermatozoa. Semen was collected from turkeys, pooled, and centrifuged to remove spermiophages and other cells. The sperm cells were then mechanically disrupted with a Dounce homogenizer, sonicated, and centrifuged using a discontinuous Percoll gradient. Electron microscopy revealed morphologically intact mitochondria. The isolated mitochondria exhibited cytochrome oxidase activity, oxygen consumption, and were stained by rhodamine 123, a fluorescent stain specific for functional mitochondria in eukaryotic cells. Mitochondrial DNA (mtDNA) was isolated and purified, and the genome was determined to be 16.457 ± 0.07 kbp. Restriction fragment patterns were identified using the endonucleases EcoR1, HindIII, and BamH1. Mitochondrial DNA was also purified from turkey liver and testis, and no differences in the restriction enzyme patterns were found between somatic and germ cell mtDNA. It is concluded that mitochondria can be isolated from spermatozoa for metabolic or genetic study.

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Uniqueness of Sperm mtDNA as Compared to Somatic mtDNA in Ram

Cited by 6 publications

References 31 publications

Sperm head decondensation, pronuclear formation, cleavage and embryonic development following intracytoplasmic injection of mitochondria-damaged sperm in mammals

Sperm head decondensation, pronuclear formation, cleavage and embryonic development following intracytoplasmic injection of mitochondria-damaged sperm in mammals

Mitochondrial DNA in somatic and germinal cells of male rats, and embryo transfer between donors and recipients of known mitochondrial DNA

Isolation and Characterization of Mitochondria from Turkey Spermatozoa

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