Unistellate spermatozoa of decapods: comparative evaluation and evolution of the morphology

Braga, André; Nakayama, Cíntia Labussière; Poersch, Luís; Wasielesky, Wilson

doi:10.1007/s00435-013-0187-2

Cited by 31 publications

(52 citation statements)

References 108 publications

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“…Braga et al. () suggested that in palaemonids, the posterior region of the main body of the sperm is in first contact with the oocyte envelope, and that the tip of the spike contains the enzymes responsible for egg incorporation. In contrast to suggestions from previous studies, we propose that the brush‐like border in the spermatozoa may be participating in sperm–egg recognition and that the spike may contain acrosomal components required for egg penetration.…”

Section: Discussionmentioning

confidence: 99%

New insights in the male anatomy, spermatophore formation, and sperm structure in Atyidae: The red cherry shrimp Neocaridina davidi

Tomás

Bento

Mutti

et al. 2019

Invertebrate Biology

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This study aims to analyze the functional anatomy of the male reproductive system in Neocaridina davidi, a very popular ornamental species of caridean shrimp. Mature males were cold‐anaesthetized and their reproductive systems were dissected for histological and histochemical analysis, while the spermatozoa and spermatophore wall ultrastructure were analyzed under transmission electron microscopy. The male reproductive system consisted of two coiled testes, which were continuous with the vasa deferentia. Testes were positioned on the dorsal side of the cephalothorax above the hepatopancreas, and comprised seminiferous tubules where spermatogenesis occurred. Each vas deferens (VD) was a long tube dorsolaterally positioned with respect to the hepatopancreas, and increased in diameter at the distal end. Three regions could be recognized in the VD: proximal, middle, and distal. The proximal region had a cylindrical epithelium with secretory cells. The middle region (or typhlosole) had a dorsal fold (or typhlosole) with a thick columnar epithelium and high secretory activity. The spermatophore was a continuous cord with three acellular layers, which were mainly characterized by the presence of neutral glycoconjugates and proteins. The sperm morphology was distinct from the inverted cup‐shaped spermatozoa observed in the majority of caridean shrimps. The spermatozoa in specimens of N. davidi were spherical in shape, with a cross‐striated, single, short spike, and arranged in clusters of three or four sperm cells. The composition of the spermatophore, and the arrangement and form of the spermatozoa, seem to be unique in comparison to other species of Caridea.

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Section: Discussionmentioning

confidence: 99%

New insights in the male anatomy, spermatophore formation, and sperm structure in Atyidae: The red cherry shrimp Neocaridina davidi

Tomás

Bento

Mutti

et al. 2019

Invertebrate Biology

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“…Crustaceans display a large array of sperm designs that are structurally different when compared with those of numerous other metazoan groups (e.g. Braga et al 2013; Fig. 1).…”

Section: Spermatozoa Extraction and Maintenancementioning

confidence: 99%

“…aflagellated and non-mobile, non-systematic occurrence of mitochondria and acrosome; review in Braga et al 2013) that are very different from the more usual entaquasperm commonly found in vertebrates or in other invertebrates, such as molluscs. This apparent diversity limits the methodological transfer of some sperm quality markers classically used, such as mobility, acrosomal membrane integrity or mitochondrial function.…”

Section: Introductionmentioning

confidence: 98%

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization

Erraud

Bonnard

Duflot

et al. 2017

Environ Sci Pollut Res

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The aim of this study was to adapt the Comet assay in spermatozoa of the marine prawn Palaemon serratus to use it as a marker of sperm quality. Indeed, due to the characteristics of their spermatozoa, the measurement of DNA integrity is one of the few markers which can be transferred to crustaceans to assess the quality of their semen. In the first step, the methods of collecting and maintaining spermatozoa were optimized. Cell survival was estimated during kinetics of preservation (i.e. 1, 2, 4 and 8 h) in various suspension media to define artificial seawater (ASW) as optimal. Several methods in the releasing of spermatozoa from the spermatophore of prawns were estimated with regard to their incidence both on the efficiency of extraction and the survival of cells. Pipetting up and down turned out to be the most successful and the least invasive technique. Secondly, the transfer of Comet assay was optimized by studying various times in both cell lysis (i.e. 1, 6, 18 h) and DNA denaturation (i.e. 15, 30 and 45 min), after in vitro exposure of spermatozoa to an H2O2 gradient as model genotoxicant. Results revealed that a minimum of 1 h in cell lysis and 15 min of DNA denaturation were sufficient to obtain valuable results, linked with a low compaction of DNA in spermatozoa of Palaemon sp. Finally, the sensitivity of P. serratus spermatozoa was assessed after in vitro exposures to model genotoxicants displaying various modes of interaction with DNA (i.e. UV-C, 13.3-79.5 J m; HO, 5-10 μM and MMS, 0.5-5 mM) and some environmental contaminants known or suspected to be genotoxic (i.e. cadmium and diuron, 0.015-1.5 μg L; carbamazepine, 0.1-10 μg L) for invertebrates. The low variability of the baseline level of DNA strand breaks recorded in controls highlighted the robustness of the method. P. serratus spermatozoa displayed significant DNA damage from the lowest doses tested for all model genotoxicants, but conversely, no genotoxic effect of tested environmental contaminants was observed. These results, which are discussed according to the protocol tested in the present study and the comparison with literature data, could suggest a difference in the response or sensitivity of spermatozoa to environmental genotoxicity between invertebrate species, and therefore the interest of Palaemonidae prawns in ecogenotoxicology. In conclusion, the present study underlines the potential of the Comet assay as a marker to assess the contamination impact on the sperm quality in Palaemonidae prawns in view to a potential application for in situ biomonitoring surveys.

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“…Dendrobranchiate sperm, like that of all decapod crustaceans, are nonmotile and consist of a spike, acrosome, and nuclear regions (reviewed by Braga et al 2013 ). Dendrobranchiate sperm have been studied most thoroughly in Sicyonia ingentis (reviewed by Clark and Griffin 1988 ).…”

Section: Spawning and Fertilizationmentioning

confidence: 98%

“Crustacea”: Decapoda (Dendrobranchiata)

Hertzler

2015

Evolutionary Developmental Biology of Invertebrates 4

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Unistellate spermatozoa of decapods: comparative evaluation and evolution of the morphology

Cited by 31 publications

References 108 publications

New insights in the male anatomy, spermatophore formation, and sperm structure in Atyidae: The red cherry shrimp Neocaridina davidi

New insights in the male anatomy, spermatophore formation, and sperm structure in Atyidae: The red cherry shrimp Neocaridina davidi

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization

“Crustacea”: Decapoda (Dendrobranchiata)

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