Unitary synaptic responses of parvalbumin interneurons evoked by excitatory neurons in the mouse barrel cortex

Canales, Alejandra; Scheuer, Katherine; Zhao, Xinyu; Jackson, Meyer B.

doi:10.1093/cercor/bhac403

Cited by 7 publications

(4 citation statements)

References 58 publications

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“…Previous hVOS studies of PV interneuron activity in somatosensory cortex determined that spike-like responses similar to action potentials had a peak ΔF/F of 2.4% (Bayguinov et al, 2017), while unitary synaptic responses elicited by an action potential in a single excitatory neuron ranged from 0.2 -0.4% (Canales et al, 2022). Assuming ~100 mV action potentials, the mean amplitude of 0.494% reported here can be estimated as approximately 21 mV.…”

Section: Discussionmentioning

confidence: 48%

Inter and Intralaminar Excitation of Parvalbumin Interneurons in Mouse Barrel Cortex

Scheuer¹,

Jansson²,

Zhao

et al. 2023

Preprint

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Parvalbumin (PV) interneurons are inhibitory fast-spiking cells with essential roles in directing the flow of information through cortical circuits. These neurons set the balance between excitation and inhibition, control rhythmic activity, and have been linked to disorders including autism spectrum and schizophrenia. PV interneurons differ between cortical layers in their morphology, circuitry, and function, but how their electrophysiological properties vary has received little attention. Here we investigate responses of PV interneurons in different layers of primary somatosensory barrel cortex (BC) to different excitatory inputs. With the genetically-encoded hybrid voltage sensor, hVOS, we recorded voltage changes simultaneously in many L2/3 and L4 PV interneurons to stimulation in either L2/3 or L4. Decay-times were consistent across L2/3 and L4. Amplitude, half-width, and rise-time were greater for PV interneurons residing in L2/3 compared to L4. Stimulation in L2/3 elicited responses in both L2/3 and L4 with longer latency compared to stimulation in L4. These differences in latency between layers could influence their windows for temporal integration. Thus PV interneurons in different cortical layers of BC show differences in response properties with potential roles in cortical computations.

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Section: Discussionmentioning

confidence: 48%

Inter and Intralaminar Excitation of Parvalbumin Interneurons in Mouse Barrel Cortex

Scheuer¹,

Jansson²,

Zhao

et al. 2023

Preprint

Self Cite

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“…In other brain regions hotspots in hVOS ΔF/F maps align well with cells in simultaneous patch clamp recordings (Ghitani et al, 2015;Bayguinov et al, 2017), as well as with cells visible in fluorescence images (Bayguinov et al, 2017;Ma et al, 2021). Furthermore, cluster analysis methods have proven effective in identifying responses arising from single parvalbumin interneurons in cortical slices, even when the cells are not visible in fluorescence images (Canales et al, 2022;Scheuer et al, 2022). It should be possible to reach this level of resolution with hVOS in VCN slices, especially if cells can be labeled more sparsely.…”

Section: Hvos Imaging With Train Stimulationmentioning

confidence: 77%

Imaging Voltage Globally and in Isofrequency Lamina in Slices of Mouse Ventral Cochlear Nucleus

Shu²,

Lin³

et al. 2023

eNeuro

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The cochlear nuclei (CN) receive sensory information from the ear and perform fundamental computations before relaying this information to higher processing centers. These computations are performed by distinct types of neurons interconnected in circuits dedicated to the specialized roles of the auditory system. In the present study we explored the use of voltage imaging to investigate CN circuitry. We tested two approaches based on fundamentally different voltage sensing technologies. Using a voltage-sensitive dye we recorded glutamate receptor-independent signals arising predominantly from axons. The mean conduction velocity of these fibers of 0.27 m/sec was rapid but in range with other unmyelinated axons. We then used a genetically-encoded hybrid voltage sensor (hVOS) to image voltage from a specific population of neurons. Probe expression was controlled using Cre recombinase linked toc-fosactivation. This activity-induced gene enabled targeting of neurons that are activated when a mouse hears a pure 15 kHz tone. In CN slices from these animals auditory nerve fiber stimulation elicited a glutamate receptor-dependent depolarization in hVOS probe-labeled neurons. These cells resided within a band corresponding to an isofrequency lamina, and responded with a high degree of synchrony. In contrast to the axonal origin of voltage-sensitive dye signals, hVOS signals represent predominantly post-synaptic responses. The introduction of voltage imaging to the CN creates the opportunity to investigate auditory processing circuitry in populations of neurons targeted on the basis of their genetic identity and their roles in sensory processing.Significance StatementThe cochlear nucleus uses dedicated circuitry to process and interpret information from the ear. This circuitry is organized tonotopically into laminae, each containing cells with an optimal sensitivity to a specific sound frequency. By targeting a genetically-encoded hybrid voltage sensor (hVOS) to identify neurons activated during the presentation of sound, the properties and function of these neurons become accessible to study in slices of mouse ventral cochlear nucleus. Imaging hVOS signals in these slices recapitulated the tonotopic organization. Imaging with a voltage sensitive dye provided a contrasting global view of signals arising predominantly from unmyelinated axons creating a potential method for studying type II auditory nerve or DCN parallel fibers.

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“…Previous hVOS studies of PV interneuron activity in somatosensory cortex determined that spike-like responses similar to action potentials had a peak ΔF/F of 2.4% [ 32 ], while unitary synaptic responses elicited by an action potential in a single excitatory neuron ranged from 0.2–0.4% [ 54 ]. Assuming ~100 mV action potentials, the mean amplitude of 0.49% reported here can be estimated as approximately 21 mV.…”

Section: Discussionmentioning

confidence: 99%

Inter and intralaminar excitation of parvalbumin interneurons in mouse barrel cortex

Scheuer,

Jansson,

Zhao

et al. 2024

PLoS ONE

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Parvalbumin (PV) interneurons are inhibitory fast-spiking cells with essential roles in directing the flow of information through cortical circuits. These neurons set the balance between excitation and inhibition and control rhythmic activity. PV interneurons differ between cortical layers in their morphology, circuitry, and function, but how their electrophysiological properties vary has received little attention. Here we investigate responses of PV interneurons in different layers of primary somatosensory barrel cortex (BC) to different excitatory inputs. With the genetically-encoded hybrid voltage sensor, hVOS, we recorded voltage changes in many L2/3 and L4 PV interneurons simultaneously, with stimulation applied to either L2/3 or L4. A semi-automated procedure was developed to identify small regions of interest corresponding to single responsive PV interneurons. Amplitude, half-width, and rise-time were greater for PV interneurons residing in L2/3 compared to L4. Stimulation in L2/3 elicited responses in both L2/3 and L4 with longer latency compared to stimulation in L4. These differences in latency between layers could influence their windows for temporal integration. Thus, PV interneurons in different cortical layers of BC respond in a layer specific and input specific manner, and these differences have potential roles in cortical computations.

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Unitary synaptic responses of parvalbumin interneurons evoked by excitatory neurons in the mouse barrel cortex

Cited by 7 publications

References 58 publications

Inter and Intralaminar Excitation of Parvalbumin Interneurons in Mouse Barrel Cortex

Inter and Intralaminar Excitation of Parvalbumin Interneurons in Mouse Barrel Cortex

Imaging Voltage Globally and in Isofrequency Lamina in Slices of Mouse Ventral Cochlear Nucleus

Inter and intralaminar excitation of parvalbumin interneurons in mouse barrel cortex

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