Units for Immunoreactive Trypsin

Wood, T; Redshaw, M. R.; Rouseli, R.H

doi:10.1016/s0140-6736(79)91240-6

Cited by 11 publications

(3 citation statements)

References 6 publications

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“…Although few specific details of this commercially prepared kit are available, Elias et al (10) have stated that a semipurified trypsin preparation (designated Ag5) was used as standard. Wood et al (28) later reported that the Ag5 standard has only 45% of the immunoreactivity of highly purified trypsin and have described a method for the conversion of trypsin values obtained with Ag5 trypsin to values for pure trypsin. However, the trypsin values obtained by these authors after conversion remain much higher than the levels reported by Borgstrom and Ohlsson (4) and by this laboratory (12).…”

Section: Discussionmentioning

confidence: 99%

Plasma Immunoreactive Pancreatic Cationic Trypsinogen in Cystic Fibrosis: a Sensitive Indicator of Exocrine Pancreatic Dysfunction

et al. 1981

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SummaryPlasma immunoreactive cationic trypsin(ogen) levels were determined in 32 control subjects and 43 patients with varying degrees of pancreatic insufficiency including 35 with cystic fibrosis (CF) and eight with Shwachman's syndrome. In six CF infants less than 2 years of age, plasma trypsin(ogen) levels were significantly elevated (97.3 + 62.2 ng/ml) above the normal range for nine controls (7.0 + 5.9 ng/ml, P < 0.025). Four of these infants had steatorrhea, three of whom had undetectable duodenal trypsin activity after stimulation with secretin-cholecystokinin. In two CF infants, molecular size fractionation by gel filtration of plasma followed by radioimmunoassay of the column fractions demonstrated that trypsinogen was the only immunoreactive species in the circulation.In contrast, in older CF patients with steatorrhea (mean age, 15.3 + 4.6 years), plasma cationic trypsin(ogen) levels were undetectable or low (1.1 2 1.7 ng/ml). This finding clearly distinguished them from older CF patients without steatorrhea (mean age, 14.3 f 3.9 years) in whom cationic trypsin(ogen) levels were significantly higher (23.3 + 17.6 ng/ml; P < 0.01). The mean trypsin(ogen) concentration in the older CF patients without steatorrhea did not differ from the mean value for 23 normal subjects of similar age. Plasma cationic trypsin(ogen) levels in two Shwachman's patients with steatorrhea (0.19 and 0.86 ng/ml) were significantly lower than the values found in six Shwachman's patients without steatorrhea (5.9 + 2.3 ng/ml; P < 0.025). Furthermore, in nine older CF patients and eight Shwachman's patients, circulating trypsin(ogen) levels were highly correlated with duodenal trypsin output after secretin-cholecystokinin stimulation (r = 0.946, P < 0.01; r = 0.899, P < 0.01, respectively). These results suggest that in CF infants high levels of circulating trypsin(ogen) persist even in those with complete pancreatic insufficiency. In older CF patients and those with Shwachman's syndrome, however, circulating trypsin(ogen) accurately reflects residual pancreatic function.

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Section: Discussionmentioning

confidence: 99%

Plasma Immunoreactive Pancreatic Cationic Trypsinogen in Cystic Fibrosis: a Sensitive Indicator of Exocrine Pancreatic Dysfunction

et al. 1981

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“…No significant differ- and 380 ng/ml, respectively. These differ ences were attributed in part to differences in the definition of the purity of standard tryp sin [10]. In the present paper, the influence of inactivation of trypsin on the immunoreac tivity of trypsin and serum immunoreactive trypsin concentration were investigated using the same purified trypsin and the same nor mal sera.…”

Section: Scrum Immunoreactive Trypsin Concentration Measured By Riamentioning

confidence: 96%

Influence of Inactivation of Trypsin on Immunoreactivity and Serum Immunoreactive Trypsin Concentration Measured by Radioimmunoassay

Iwaki¹,

Ogawa²,

Kitahara³

et al. 1983

Enzyme

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The influences of various active site-specific reagents of trypsin and protease inhibitors on the immunoreactivity of trypsin and serum trypsin concentration have been studied by radioimmunoassay (RIA). The RIA using inactivated125I-trypsin as tracer showed lower B(0)/T than the RIA using active 125I-trypsin, but the coefficient of variance of the former was smaller than that of the latter. Normal serum trypsin concentrations were 26.12- 36.38 ng/ml with the RIA using inactivated 125I-trypsin as antigen tracer, and 201.15 ng/ml with the RIA using active 125I-trypsin as tracer. The recovery experiment showed that the difference was due to the interaction of serum protease inhibitors and labeled active trypsin.

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“…The normal cationic trypsin level, for example, was determined to be 273 ng/ml by the RIA system established by Elias et al [56] but quite different values were obtained in other studies [13,53]. Woods et al [57] later sug gested that the standard protein used in this RIA system was a crude trypsin, and that the normal value obtained using a highly puri fied agent was 45% (122 ng/ml) of this value.…”

Section: Molecular Forms In Serum and Immunoreactivitymentioning

confidence: 86%

Immunoassay of Circulating Enzymes: Current Status

Ogawa¹

1986

Enzyme

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This report reviews the current status of immunoassays for circulating enzymes. The necessity for measuring the protein concentration of circulating enzymes is evident in the light of the inability of conventional enzyme assays to measure the levels of released enzymes accurately, especially when these enzymes are inactivated, bound to an inhibitor, or present as proenzymes. In some cases, therefore, measurement of enzyme protein concentrations rather than their catalytic activities provide more relevant information for detection of lesions and evaluation of their extent in the organs from which the enzymes are derived. Facts to be considered in the measurement and interpretation of the values obtained by immunoassays are discussed.

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Units for Immunoreactive Trypsin

Cited by 11 publications

References 6 publications

Plasma Immunoreactive Pancreatic Cationic Trypsinogen in Cystic Fibrosis: a Sensitive Indicator of Exocrine Pancreatic Dysfunction

Plasma Immunoreactive Pancreatic Cationic Trypsinogen in Cystic Fibrosis: a Sensitive Indicator of Exocrine Pancreatic Dysfunction

Influence of Inactivation of Trypsin on Immunoreactivity and Serum Immunoreactive Trypsin Concentration Measured by Radioimmunoassay

Immunoassay of Circulating Enzymes: Current Status

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