Universal point-of-care detection of proteins based on proximity hybridization-mediated isothermal exponential amplification

Abstract

We have developed a point-of-care (POC) lateral flow biosensor (LFB) for universal protein detection based on a proximity hybridization-mediated protein-to-DNA signal transducer, isothermal exponential amplification (EXPAR) and catalytic hairpin assembly...

Recent advances in cascade isothermal amplification techniques for ultra-sensitive nucleic acid detection

“…Copyright © The Royal Society of Chemistry 2019. (B) Schematic illustration for protein detection based on proximity hybridization-mediated isothermal exponential amplification [ 115 ]. (C) Schematic illustration of electrochemical assay for miRNA based on CRISPR-Cas13a system and CHA reaction [ 116 ].…”

“…Tang et al [ 115 ] developed a universal protein detection method based on a protein-to-DNA signal transducer, by cascading EXPAR and CHA ( Fig. 6 B).…”

One-tube B7-H3 detection based on isothermal exponential amplification and dendritic hybridization chain reaction

“…The signal translator was recently applied in proteins and small molecular detection with high specicity. 17,18 Isothermal amplication has attracted a great deal of research interest because of its simplicity, portability, short assay time, and cost-effectiveness. 19,20 Isothermal exponential amplication (EXPAR) is an isothermal nucleic acid amplication technique with excellent amplication efficiency.…”

“…The signal translator was recently applied in proteins and small molecular detection with high specificity. 17,18…”

“…23–27 Detection approaches based on EXPAR and catalytic hairpin assembly (CHA) have widely been applied in microRNA and protein assays. 17,28…”