Universally Primed Polymerase Chain Reaction Alleles and Internal Transcribed Spacer Restriction Fragment Length Polymorphisms Distinguish Two Subgroups in <i>Botrytis aclada</i> Distinct from <i>B. byssoidea</i>

Nielsen, Karsten; Justesen, Annemarie Fejer; Jensen, Dan Funck; Yohalem, D.

doi:10.1094/phyto.2001.91.6.527

Cited by 40 publications

(21 citation statements)

References 17 publications

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“…Variation in the size of conidia from different isolates of Botrytis sp. was not uncommon (Nielsen et al, 2001). Fungal morphological variation is highly subjected to the substrate availability and experimental conditions, and often exponentially responds to minor change in these conditions.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Botrytis cinerea isolates from chickpea: DNA polymorphisms, cultural, morphological and virulence characteristics

Suresh¹,

e²,

Sharma³

et al. 2010

Afr. J. Biotechnol.

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Eight isolates of Botrytis cinerea, causal organism of chickpea from eight different locations of western and eastern Indo-Gangetic Plains (IGP) of India were analyzed for morphological, cultural, virulence and genetic variations. Characterization of virulence and genetic variations of the isolates was based on their pathogenicity against 40 selected chickpea genotypes and randomly amplified polymorphic DNA (RAPD) analysis, respectively. The isolates differed in their optimum growth, temperature, conidial formation and size of conidia on potato dextrose agar. Based on cluster analysis, the eight test isolates were separated into three pathotypes with two isolates from western and four from eastern IGP grouped together with >80% similarity. Based on cluster analysis of the RAPD banding patterns, genetic similarity of the isolates varied from 14-44%, and the isolates were separated into three groups. However, pathotypes variation detected among B. cinerea isolates could not be differentiated based on the RAPD markers examined.

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Section: Discussionmentioning

confidence: 99%

Characterization of Botrytis cinerea isolates from chickpea: DNA polymorphisms, cultural, morphological and virulence characteristics

Suresh¹,

e²,

Sharma³

et al. 2010

Afr. J. Biotechnol.

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“…Evidence of this type has been published for two other ascomycete fungi which associate with plant hosts. Botrytis allii , the only other known amphihaploid (‘polyploid’ in the less specific terminology used in the relevant papers) plant pathogen, arose through interspecific hybridization between the haploid species B. aclada and B. byssoidea (Nielsen & Yohalem, 2001; Nielsen et al ., 2001; Yohalem et al ., 2003). Neotyphodium species are asexual endophytes of grasses.…”

Section: Introductionmentioning

confidence: 99%

Direct molecular evidence supports long‐spored microsclerotial isolates of Verticillium from crucifers being interspecific hybrids

2008

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There is no published evidence directly supporting the suggestion that long-spored isolates of Verticillium dahliae are interspecific hybrids. Retention in the nuclear genome of long-spored (amphihaploid) isolates of two sequence types where non-hybrid short-spored (haploid) isolates carry only single sequence types would provide such evidence. PCR amplicons for a partial β -tubulin gene and a 5 S rRNA-associated sequence were cloned and sequenced to provide multiple sequences for individual isolates. For the 5 S rRNA sequence, two sequence types were found for long-spored isolates, but only one for the haploid isolates. For the β -tubulin gene, two or three sequence types were found in longspored isolates, but only one in most haploid isolates. One haploid isolate gave two sequence types, one like that from the other haploid isolates and the second like the short-sequence type from some long-spored isolates. These results support the long-spored isolates being interspecific hybrids between a 'parental' species similar to V. dahliae (haploid) and an unidentified second species. The presence of a sequence type in some long-spored isolates similar to that in one haploid isolate suggests a third 'parental' species may have been involved, possibly via a short-spored isolate that was itself hybrid.

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“…Two subgroups within B. aclada (AI and AII) can be distinguished based on chromosome number and conidial dimensions (20,58). Polymorphic polymerase chain reaction (PCR) alleles and internal transcribed spacer restriction fragment length polymorphisms (ITS-RFLPs) have been used to demonstrate that isolates in subgroups AI and AII are distinct from B. byssoidea (36,39). In fact, Nielsen and Yohalem (38) concluded that the largerspored subgroup developed as a result of a hybridization event between a smallspored isolate of B. aclada and an isolate of B. byssoidea.…”

mentioning

confidence: 99%

“…Neck rot species of Botrytis are found in all areas of the world where onions are produced, but the greatest losses have been reported in temperate regions, where B. allii and B. aclada appear to be the predominant onion neck rot pathogens (8, 11,13,28,36). However, the two species are difficult to distinguish morphologically, with similar growth patterns on agar media and overlapping spore sizes (7, 76).…”

mentioning

confidence: 99%

A Real-Time, Quantitative PCR Seed Assay for Botrytis spp. that Cause Neck Rot of Onion

Chilvers

Toit²,

Akamatsu

et al. 2007

Plant Disease

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A real-time fluorescent polymerase chain reaction (PCR) assay was developed using SYBR Green chemistry to quantify the Botrytis spp. associated with onion (Allium cepa) seed that are also able to induce neck rot of onion bulbs, i.e., B. aclada, B. allii, and B. byssoidea. The nuclear ribosomal intergenic spacer (IGS) regions of target and nontarget Botrytis spp. were sequenced, aligned, and used to design a primer pair specific to B. aclada, B. allii, and B. byssoidea. Primers and amplification parameters were optimized to avoid amplifying the related species B. cinerea, B. porri, and B. squamosa, as well as Sclerotinia sclerotiorum and isolates of 15 other fungal species commonly found associated with onion seed. The primers reliably detected 10 fg of genomic DNA per PCR reaction extracted from pure cultures of B. aclada and B. allii. Conventional assays of surface-disinfested and nondisinfested seed on an agar medium were used to determine the incidence of neck rot Botrytis spp. associated with each of 23 commercial onion seed lots, and the real-time PCR assay was used to determine the quantity of DNA of neck rot Botrytis spp. in each seed lot. A linear relationship could not be found between the incidence of seed infected with the neck rot Botrytis spp. using the conventional agar seed assays and the quantity of DNA of the neck rot Botrytis spp. detected by the real-time PCR assay. However, the real-time PCR assay appeared to be more sensitive than the conventional agar assay, allowing detection of neck rot Botrytis spp. in 5 of the 23 seed lots that tested negative using the conventional agar seed assay.

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Universally Primed Polymerase Chain Reaction Alleles and Internal Transcribed Spacer Restriction Fragment Length Polymorphisms Distinguish Two Subgroups in Botrytis aclada Distinct from B. byssoidea

Cited by 40 publications

References 17 publications

Characterization of Botrytis cinerea isolates from chickpea: DNA polymorphisms, cultural, morphological and virulence characteristics

Characterization of Botrytis cinerea isolates from chickpea: DNA polymorphisms, cultural, morphological and virulence characteristics

Direct molecular evidence supports long‐spored microsclerotial isolates of Verticillium from crucifers being interspecific hybrids

A Real-Time, Quantitative PCR Seed Assay for Botrytis spp. that Cause Neck Rot of Onion

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