2018
DOI: 10.1063/1.5050906
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Unlimited acquisition time in electron holography by automated feedback control of transmission electron microscope

Abstract: The signal-to-noise ratio of measurements by electron holography could be considerably improved if longer exposure times were possible: increasing the number of electrons contributing to the hologram improves the counting statistics. However, instrumental instabilities causing drift in the hologram fringes and specimen position make acquisition times of above a few seconds counterproductive. The current approach is to acquire image stacks of holograms, with short exposure times, followed by numerical realignme… Show more

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Cited by 31 publications
(22 citation statements)
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“…The fringe spacing is equal to 1 nm (7 pixels) allowing for a spatial resolution of 1.5 nm for the treated magnetic phase images. The exposure time was set to 1 nm using dynamic automation acquisition for removing instabilities and applying the fringe π-shift method 66,67 . Phase and amplitude images were extracted from the holograms by using homemade software based on fast-Fourier transform approach.…”
Section: Methodsmentioning
confidence: 99%
“…The fringe spacing is equal to 1 nm (7 pixels) allowing for a spatial resolution of 1.5 nm for the treated magnetic phase images. The exposure time was set to 1 nm using dynamic automation acquisition for removing instabilities and applying the fringe π-shift method 66,67 . Phase and amplitude images were extracted from the holograms by using homemade software based on fast-Fourier transform approach.…”
Section: Methodsmentioning
confidence: 99%
“…In this section we will show how the use of image stacks previously introduced in conventional holography experiments [24,25], can contribute to overcome this limitation.…”
Section: Detection Limits In Off-axis Electron Holography With Femtosmentioning
confidence: 99%
“…Data processing has been performed using home- In the Not corrected row, individual holograms in the stack have been summed without applying any fringe drift using the gun tilt coils will be implemented in the future [25]. The future transfer of our technology from the old HF2000 to a recent stateof-the-art TEM, will give us the possibility to move towards this direction and the use of direct electron camera providing a higher DQE should also strongly improve the live-correction process.…”
Section: Detection Limits In Off-axis Electron Holography With Femtosmentioning
confidence: 99%
“…In cryo-TEM, feedback from image analysis is employed to identify and judge the quality of particles for acquisition of imaging [10][11][12] or diffraction data [13][14][15] . In material science TEM, similar approaches exist that enable ultrastable imaging [16][17][18] . There is, however, no solution that incorporates advanced image analysis procedures into the various kinds of TEM data acquisition workflows in a generic way.…”
Section: Introductionmentioning
confidence: 99%