Entrepreneurship education can increase student’s entrepreneurial skills and intention; and entrepreneurship activities stimulate economic growth. Therefore, the number of entrepreneurship courses is increasing, but they are often offered to students from a specific field of study and/or to only one educational level, even though multidisciplinarity is important for entrepreneurship. This study has carried out an exploratory single case study on an entrepreneurial programme in a multidisciplinary environment, that is, the Contamination Lab of Turin (CLabTo). Moreover, pre- and post-surveys have also been conducted in order to perform some qualitative analyses. The results show the importance of creating teams with different competencies, cognitive and decision-making skills. Moreover, we explain how design-thinking is useful in entrepreneurship education and that a challenge-based entrepreneurship course leads to cooperation with external actors in the local entrepreneurial ecosystem. It is also explained what the obstacles and opportunities of these programmes are and how to improve them. Moreover, on the basis of pre- and post-surveys, our qualitative analyses show that the students’ overall perception of their abilities to work in a multidisciplinary team, their entrepreneurial skills and entrepreneurial intentions increased slightly. In short, this work is aimed at increasing the importance of entrepreneurship education in a multidisciplinary environment and the use of practical-oriented teaching models.