Unlocking the secrets of Brassica oleracea crops : A genomic journey

Abstract

Theophrastrus , suggesting that domestication of B. oleracea can date back to as early as around 400 BC. Genome sequencing Revolutions of sequencing technologyThe past few decades have seen several rounds of technological revolution in DNA sequencing. The first revolution came with the advent of Sanger sequencing, which allows deciphering of complete genome sequences for the first time (Sanger et al., 1977. This technology was considered the gold standard for DNA sequencing for the next 25 years since 1977 (Sanger et al., 1977, Grada and Weinbrecht, 2013). Its read lengths typically range between 600 and 800 bp and the base accuracy is high with only 0.01% error rate (Mardis, 2017). However, the major drawback is that this technology is too labour-intensive, time-consuming and expensive for large-scale sequencing projects (Van Dijk et al., 2018). Sanger sequencing was used in the 13-year-long (1990-2003) and very expensive ($3 billion costs)