The aboveground biomass (AGB) of summer maize is an important indicator for assessing crop growth status and predicting yield, playing a significant role in agricultural management and decision-making. Traditional on-site measurements of AGB are limited, due to low efficiency and a lack of spatial information. The development of unmanned aerial vehicle (UAV) technology in agriculture offers a rapid and cost-effective method for obtaining crop growth information, but currently, the prediction accuracy of summer maize AGB based on UAVs is limited. This study focuses on the entire growth period of summer maize. Multispectral images of six key growth stages of maize were captured using a DJI Phantom 4 Pro, and color indices and elevation data (DEM) were extracted from these growth stage images. Combining measured data such as summer maize AGB and plant height, which were collected on the ground, and based on the three machine learning algorithms of partial least squares regression (PLSR), random forest (RF), and long short-term memory (LSTM), an input feature analysis of PH was carried out, and a prediction model of summer maize AGB was constructed. The results show that: (1) using unmanned aerial vehicle spectral data (CIS) alone to predict the biomass of summer maize has relatively poor prediction accuracy. Among the three models, the LSTM (CIS) model has the best simulation effect, with a coefficient of determination (R2) ranging from 0.516 to 0.649. The R2 of the RF (CIS) model is 0.446–0.537. The R2 of the PLSR (CIS) model is 0.323–0.401. (2) After adding plant height (PH) data, the accuracy and stability of model prediction significantly improved. R2 increased by about 25%, and both RMSE and NRSME decreased by about 20%. Among the three prediction models, the LSTM (PH + CIS) model had the best performance, with R2 = 0.744, root mean square error (RSME) = 4.833 g, and normalized root mean square error (NRSME) = 0.107. Compared to using only color indices (CIS) as the model input, adding plant height (PH) significantly enhances the prediction effect of AGB (aboveground biomass) prediction in key growth periods of summer maize. This method can serve as a reference for the precise monitoring of crop biomass status through remote sensing with unmanned aerial vehicles.
