Purpose: The widespread use of ageist language is generally accepted as commonplace and routine in most cultures and settings. In order to disrupt ageism, we must examine the use of ageist language and sentiments among those on the front line of providing advocacy, services, and policy for older adults; the professional culture of the aging services network. The recorded video segments from the sixth White House Conference on Aging (WHCOA) provided a unique opportunity to examine discourse used by professionals and appointed representatives in the field of aging within a professional sociocultural context. Design and Methods: A qualitative discourse analysis of video recordings was used to analyze the 15 video fragments that comprised the recorded sessions of the 2015 WHCOA. Results: About 26 instances were identified that captured statements expressing personal age, aging or an age-related characteristic negatively in regard to self or other (microageism), and/or statements expressing global negative opinions or beliefs about aging and older adults based on group membership (macroageism). A theoretical pathway was established that represents the dynamic process by which ageist statements were expressed and reinforced (relational ageism). Implications: Numerous instances of ageism were readily identified as part of a live streamed and publically accessible professional conference attended and presented by representatives of the aging services network. To make meaningful gains in the movement to disrupt ageism and promote optimal aging for all individuals, we must raise awareness of the relational nature, expression, and perpetuation of ageism.