Unmyelinated Nerve Fiber Analysis of the Human Oculomotor Nerve

Kawamura, Noriyoshi; Matsumoto, Kiyoshi; Wada, Akira; Goto, Noboru

doi:10.2535/ofaj1936.77.2-3_59

Cited by 6 publications

(4 citation statements)

References 11 publications

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“…Several morphological, physiological, biochemical and ophthalmological studies have been conducted to investigate age‐related changes on visual system (Koicheva and Zlateva, 1980; Toshida et al., 1993; Pratt et al., 1997; Sun et al., 1997; Munoz et al., 1998; Kawamura et al., 2000; Chen et al., 2003; Oguro et al., 2004). Koicheva and Zlateva (1980) reported the decrease on visual functions (fine distinguishing details, colour and relief) while Sun et al.…”

Section: Introductionmentioning

confidence: 99%

Age‐Related Immunohistochemical and Ultrastructural Changes in Rat Oculomotor Nerve

Bahçelioğlu¹,

Elmas

Kürkçüoğlu

et al. 2008

Anat Histol Embryol

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During ageing process, multiple changes occur on nervous tissue composed of cells and extracellular matrix. Changes on nervous tissues are usually known as degenerative changes on axon structure and connective tissue covering the nerve such as a decrease in the number of fibre or general structural changes. For this purpose, we have studied age-dependent ultrastructural changes in the rat oculomotor nerve with electron microscopy and also demonstrated collagen structure of the neural sheaths with immunohistochemical techniques. This study was conducted in Gazi University Faculty of Medicine, Department of Anatomy with a total of nine Wistar albino rats. We observed strong collagen type I immunoreactivity in endoneurium and slight to moderate reactivity in fibroblast cytoplasm in 3-month- and 12-month-old groups and mild reactivity in 24-month-old group. Collagen type IV immunoreactivity was stronger in endoneurium and perineurium in the 3-month- and 12-month-old groups compared with collagen type I and fibroblast cytoplasm showed a very strong reactivity. On the other hand, in the 24-month-old group, there was slight reactivity in endoneurium and a strong reactivity in perineurium. NGF staining showed moderate to strong reactivity on Schwann cells of the 3-month-old group. The immunoreactivity decreased in the 12-month- and 24-month-old groups. In the 3-month-old rat group, Schwann cell cytoplasm, mitochondrial structure and neurofilaments were normal. In the 12-month-old group, there were no changes in organelle distribution, mitochondrial structure and neurofilaments, but there was an increase in the connective tissue. An inconsiderable number of degenerated myelinated nerves were observed. We detected an important decrease in the collagen type I immunoreactivity, which could suggest that the endoneurium, perineurium and epineurium are less resistant to the age-related collagen loss and that the peripheral nerve is protected by a weaker barrier in the old group. The collagen type IV immunoreactivity was significantly decreased with age. NGF synthesis decreases with age because of Schwann cell structural degeneration or for different reasons. Thus, this could explain the diminished capacity of regeneration and damage of the myelination of the peripheral nerve.

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Section: Introductionmentioning

confidence: 99%

Age‐Related Immunohistochemical and Ultrastructural Changes in Rat Oculomotor Nerve

Bahçelioğlu¹,

Elmas

Kürkçüoğlu

et al. 2008

Anat Histol Embryol

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“…The shrinkage ratios during the preparation of histology sections amounted to 10 + 0% in length according to our researches2-4, [6][7][8]11,12,[15][16][17][18][19][20][21].…”

Section: Methodsmentioning

confidence: 85%

“…Up to now, however, no staining method was available for unmyelinated nerve fibers, except for transmission electron microscopy. Reports on the evaluation of nerve fibers1-21) are rather limited in numbers except for our contri-butions2-4, [6][7][8]11,12,[15][16][17][18][19][20][21].The purpose of this paper is to introduce two discriminative staining methods with discussion on the modifications required in order to differentiate the various types of nerve fibers described above. These methods not only differentiate various nerve fibers, but also produce good results from the view point of morphometry.…”

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confidence: 99%

Morphological Differentiation of Nerve Fibers: Central, Peripheral, Myelinated and Unmyelinated

Goto

Ezure

et al. 2001

Okajimas Folia Anatomica Japonica

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We have developed a new technique for the morphological differentiation of various nerve fibers which is especially suitable for the morphometric study of nerve fibers of the human nervous system with the help of an image-analyzer. The knowledge from findings by this technique, which is based on several study methods, may be of importance in promoting further neuromorphologic studies and in properly understanding various aspects of neurological symptomatology and the aging process of the nervous system including nerve fibers.

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“…Unmyelinated nerve fibers of the oculomotor nerve have occasionally been observed in experimental animals with the use of electron microscopes, but no details concerning normal oculomotor nerves in humans had been published. An analysis of unmyelinated nerve fibers in the human oculomotor nerve was conducted using the new methods mentioned above 61 . The authors studied the numbers and transverse areas of unmyelinated fibers of the oculomotor nerve in 20 cadavers.…”

Section: Miscellaneousmentioning

confidence: 99%

Morphometric evaluations of the human nervous system

Goto

2006

Human Cell

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It is very important to evaluate and accurately understand the various conditions of the human nervous system. In this review article, we introduce several morphometric reports that are proven to be accurate from the view point of various errors (range of tissue shrinkage ratios, microscopic multiple counting, artifacts of microscopic structures, etc.). We review the following aspects of the selected reports: methodology, developmental research, neuronal differences, gender differences, aging process and miscellaneous (nerve fibers, unmyelinated fibers, in relation to neuropathology, clinical image-analysis and immunohistochemistry).

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Unmyelinated Nerve Fiber Analysis of the Human Oculomotor Nerve

Cited by 6 publications

References 11 publications

Age‐Related Immunohistochemical and Ultrastructural Changes in Rat Oculomotor Nerve

Age‐Related Immunohistochemical and Ultrastructural Changes in Rat Oculomotor Nerve

Morphological Differentiation of Nerve Fibers: Central, Peripheral, Myelinated and Unmyelinated

Morphometric evaluations of the human nervous system

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