2023
DOI: 10.1021/acsomega.2c04462
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Unpacking the Proteome and Metaproteome of the Black Soldier Fly Larvae: Efficacy and Complementarity of Multiple Protein Extraction Protocols

Abstract: The larvae of the black soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), have demonstrated the ability to efficiently bioconvert organic waste into a sustainable source of food and feed, but fundamental biology remains to be discovered to exploit their full biodegradative potential. Herein, LC−MS/MS was used to assess the efficiency of eight differing extraction protocols to build foundational knowledge regarding the proteome landscape of both the BSF larvae body and gut. Each protocol yielded co… Show more

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Cited by 2 publications
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“…For example, the number of proteins identified varies widely among treated wastewater effluents produced by different wastewater treatment plants (WWTPs), with generally less than a thousand identified by metaproteomics compared to the actual number of proteins which is estimated to be in the range of hundreds of thousands up to millions [9,10]. Moreover, different protein extraction methods lead to variations in protein recovery and introduce bias when analyzing the microbial communities, therefore, new protocols for metaproteomics are proposed to improve the yield of extraction, to facilitate the entire workflow from sample processing to data analytics, and to enhance the resolution of metaproteome analysis [11][12][13][14][15]. In particular, protein extraction and sample processing is a subject of improvement by the scientific community in the Metaproteomics Initiative [4,16].…”
Section: Introductionmentioning
confidence: 99%
“…For example, the number of proteins identified varies widely among treated wastewater effluents produced by different wastewater treatment plants (WWTPs), with generally less than a thousand identified by metaproteomics compared to the actual number of proteins which is estimated to be in the range of hundreds of thousands up to millions [9,10]. Moreover, different protein extraction methods lead to variations in protein recovery and introduce bias when analyzing the microbial communities, therefore, new protocols for metaproteomics are proposed to improve the yield of extraction, to facilitate the entire workflow from sample processing to data analytics, and to enhance the resolution of metaproteome analysis [11][12][13][14][15]. In particular, protein extraction and sample processing is a subject of improvement by the scientific community in the Metaproteomics Initiative [4,16].…”
Section: Introductionmentioning
confidence: 99%