Unpollinated Ovaries Used to Produce Doubled Haploid Lines in Durum Wheat

Ayed-Slama, Olfa; Amara, Hajer Slim

doi:10.1007/978-1-0716-1315-3_12

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…In vitro gynogenesis is a second method of haploid production used in several agriculturally essential crops such as maize, barley, and wheat [72]. This method has proven significant in overcoming the limitation of androgenesis in producing a higher ratio of albino plants and male-sterile genotypes [6,73,74]. Furthermore, the in vitro gynogenesis method is used with salt stress (100 mM) during callus proliferation to create genetic variability among regenerated plantlets to permit the selection of salt-tolerant lines derived from durum wheat [75].…”

Section: Gynoclonal Variationmentioning

confidence: 99%

“…Plant tissue culture includes a culture of the cell protoplast, anther and microspore (immature pollen grain), ovary and ovules, and embryo, which features genetic and epigenetic variation in the breeding material. Such in vitro culture methods exploit all the available genetic variability and reduce the period of the breeding program to develop tolerant and resistant genotypes [6]. Primarily, plant tissue culture is used in vegetatively propagated crops and self-pollinated crops, especially with narrower genetic bases.…”

Section: Introductionmentioning

confidence: 99%

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Applications of In Vitro Tissue Culture Technologies in Breeding and Genetic Improvement of Wheat

Wijerathna-Yapa

Ramtekey

Ranawaka

et al. 2022

Plants

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Sources of new genetic variability have been limited to existing germplasm in the past. Wheat has been studied extensively for various agronomic traits located throughout the genome. The large size of the chromosomes and the ability of its polyploid genome to tolerate the addition or loss of chromosomes facilitated rapid progress in the early study of wheat genetics using cytogenetic techniques. At the same time, its large genome size has limited the progress in genetic characterization studies focused on diploid species, with a small genome and genetic engineering procedures already developed. Today, the genetic transformation and gene editing procedures offer attractive alternatives to conventional techniques for breeding wheat because they allow one or more of the genes to be introduced or altered into an elite cultivar without affecting its genetic background. Recently, significant advances have been made in regenerating various plant tissues, providing the essential basis for regenerating transgenic plants. In addition, Agrobacterium-mediated, biolistic, and in planta particle bombardment (iPB) gene delivery procedures have been developed for wheat transformation and advanced transgenic wheat development. As a result, several useful genes are now available that have been transferred or would be helpful to be transferred to wheat in addition to the current traditional effort to improve trait values, such as resistance to abiotic and biotic factors, grain quality, and plant architecture. Furthermore, the in planta genome editing method will significantly contribute to the social implementation of genome-edited crops to innovate the breeding pipeline and leverage unique climate adaptations.

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Section: Gynoclonal Variationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Applications of In Vitro Tissue Culture Technologies in Breeding and Genetic Improvement of Wheat

Wijerathna-Yapa

Ramtekey

Ranawaka

et al. 2022

Plants

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Production of Gynogenic Plants of Red Beet (Beta vulgaris L.) in Unpollinated Ovule Culture In Vitro

Zayachkovskaya

Домблидес

Zayachkovsky

et al. 2021

Plants

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The unique and balanced components of the biochemical composition, together with high antioxidant activity, make the red beet necessary a dietary vegetable crop, much contributing to healthy food ration. The application of the technology for producing gynogenic plants in vitro increases the genetic diversity and significantly reduces the period of time required to obtain the appropriate homozygous lines used to create the F1 hybrids that are demanded in the market. For induction of gynogenesis, we used IMB medium developed by us with the addition of 55 g/L sucrose, 3 g/L phytogel, 200 mg/L ampicillin, and 0.4 mg/L thidiazuron (TDZ) and cultured at 28 °C in the dark for 4–6 weeks. Shoot regeneration from embryoids and callus was performed on MS medium with 20 g/L sucrose, 3 g/L phytogel, 1 mg/L 6-benzylaminopurine (BAP), and 0.1 mg/L gibberellic acid (GA3). Immersion of the obtained microshoots with 5–7 well-developed leaves for 10–15 s into concentrated sterile indole-3-butyric acid (IBA) solution (50 mg/L) followed by their cultivation on solid medium ½ IMB with 2% sucrose and 3 g/L phytogel was the most efficient method for root formation. The addition of silver nitrate (22 mg/L) to the nutrient medium provoked an increase in the number of induced ovules up to nine per Petri dish (up to 25% of induced ovules). Gynogenic development was produced in six out of 11 genotypes studied, and the plants that were then acclimatized to ex vitro conditions were obtained in three genotypes (Nezhnost’, Dobrynya, b/a 128). The evaluation of ploidy of gynogenic plants that was carried out by flow cytometry and direct counting of chromosomes stained with propion-lacmoide revealed that all obtained gynogenic plants were haploids (2n = x = 9).

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Unpollinated Ovaries Used to Produce Doubled Haploid Lines in Durum Wheat

Cited by 2 publications

References 13 publications

Applications of In Vitro Tissue Culture Technologies in Breeding and Genetic Improvement of Wheat

Applications of In Vitro Tissue Culture Technologies in Breeding and Genetic Improvement of Wheat

Production of Gynogenic Plants of Red Beet (Beta vulgaris L.) in Unpollinated Ovule Culture In Vitro

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