Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology

Schoenborn, Andreas; Schmid, Pascal; Bräm, Sarah; Reifferscheid, Georg; Ohlig, Marina; Buchinger, Sebastian

doi:10.1016/j.chroma.2017.11.009

Cited by 32 publications

(27 citation statements)

References 23 publications

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“…Cell density was measured with a microtiter plate absorbance reader (BioTek Synergy 2, Winooski, VT, USA) at 600 nm. Yeast were prepared for L-YES and P-YES in exposure media according to an ISO standard [10] and Schönborn et al [17].…”

Section: Estrogen Screeningmentioning

confidence: 99%

“…S8), and when testing migrates of FCM. In these cases, HPTLC plates were developed to 80 mm with an Automated Multiple Development 2 (AMD2, CAMAG) using preconditioning with 1.2% NH 3 , isocratic chromatography with a solvent mixture of chloroform:acetone:petroleum ether 11:5:5 [17,29], and dried under vacuum for 2 min. For experiments described in this study, P-YES was performed without chromatography unless otherwise specified (as "P-YES with chromatography").…”

Section: L-yesmentioning

confidence: 99%

“…Performance of the P-YES was based on Schönborn et al [17]. Yeast was adjusted to 1000 ± 200 FAU in fresh exposure medium.…”

Section: P-yesmentioning

confidence: 99%

“…Based on previous publications [17,33] and our own preliminary work, the most sensitive methods, i.e., appropriate yeast strain, for performing L-YES and P-YES were used to analyze chemicals associated with FCMs. The reference compound, E2, was more potent in P-YES than L-YES by about 10-fold ( Fig.…”

Section: Quality Control and Data Analysismentioning

confidence: 99%

“…The yeast estrogen screen is one of the most established assays in HPTLC format [12-14, 17, 18]. Referred to here as planar-YES (P-YES, and specifying with or without chromatography), it has been used for analyses including wastewater [14,15], river sediment [14], herbal extracts [19], and personal care products [12], but until recently is still undergoing development in data collection [17,18] and evaluation [20,21].…”

Section: Introductionmentioning

confidence: 99%

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Estrogenic activity of food contact materials—evaluation of 20 chemicals using a yeast estrogen screen on HPTLC or 96-well plates

et al. 2020

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Food contact materials (FCM) may contain complex mixtures of estrogenic chemicals. A yeast estrogen screen performed on high performance thin-layer chromatography plates (planar-YES, P-YES) is promising for analysis of such mixtures, as it could allow for better elucidation of effects compared with established methods in microtiter plates. However, the P-YES has not been directly compared with established methods. We compared the performance of a microtiter plate YES (lyticase-YES, L-YES) to P-YES on silica gel HPTLC plates using 17β-estradiol (E2), 20 chemicals representative of migrants from plastic FCM, and three migrates of coated metal food cans. Effective doses (ED10, ED50) and estradiol equivalencies were calculated for each chemical. Thirteen chemicals had calculable EDs in the L-YES or P-YES, with average EDs 13-fold (range 0.63–36) more potent in P-YES than in the L-YES. Normalized to E2, the median estrogenicity was within 1.5-fold (0.43–8.8) between the assays. Therefore, P-YES was as or more sensitive than L-YES but potencies relative to E2 were comparable between assays. With chromatography, the P-YES detected estrogenicity in coated metal cans, effects that were unmeasurable in L-YES. With the sample preparation methods used in this study, both YES assays are sufficiently sensitive to detect bisphenol A below the specific migration limit for plastic packaging (0.05 mg/kg food). This study demonstrates that P-YES outperforms L-YES because it is more sensitive, provides comparable estradiol equivalents, and circumvents confounding mixture effects. The P-YES will be useful for routine monitoring of FCM and toxicant identification in problematic materials.

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Section: Estrogen Screeningmentioning

confidence: 99%

Section: L-yesmentioning

confidence: 99%

“…Performance of the P-YES was based on Schönborn et al [17]. Yeast was adjusted to 1000 ± 200 FAU in fresh exposure medium.…”

Section: P-yesmentioning

confidence: 99%

Section: Quality Control and Data Analysismentioning

confidence: 99%