Unpredictable Chronic Stress Alters Adenosine Metabolism in Zebrafish Brain

Zimmermann, Fernanda Francine; Altenhofen, Stefani; Kist, Luiza Wilges; Leite, Carlos Eduardo; Bogo, Maurı́cio Reis; Cognato, Giana P.; Bonan, Carla Denise

doi:10.1007/s12035-015-9270-7

Cited by 31 publications

(12 citation statements)

References 78 publications

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“…The CUMS paradigm on zebrafish was followed previous studies. Stressors were presented randomly twice a day for a total of 28 days to avoid habituation [38][39][40]. The stressors included low water to expose the dorsal body wall to the air (2 min), crowding in a 250 mL beaker containing only 150 mL of water (30 min), chasing with a net (8 min), cooling the water to 23 • C, heating the water to 33 • C (30 min), tank change, restraint stress and predator stress.…”

Section: Cums Modelmentioning

confidence: 99%

A Reliable High-Throughput Screening Model for Antidepressant

Zhang

Qiao

Liu

et al. 2021

IJMS

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Depression is the most frequent affective disorder and is the leading cause of disability worldwide. In order to screen antidepressants and explore molecular mechanisms, a variety of animal models were used in experiments, but there is no reliable high-throughput screening method. Zebrafish is a common model organism for mental illness such as depression. In our research, we established chronic unpredictable mild stress (CUMS) models in C57BL/6 mice and zebrafish; the similarities in behavior and pathology suggest that zebrafish can replace rodents as high-throughput screening organisms. Stress mice (ip., 1 mg/kg/d, 3 days) and zebrafish (10 mg/L, 20 min) were treated with reserpine. As a result, reserpine caused depression-like behavior in mice, which was consistent with the results of the CUMS mice model. Additionally, reserpine reduced the locomotor ability and exploratory behavior of zebrafish, which was consistent with the results of the CUMS zebrafish model. Further analysis of the metabolic differences showed that the reserpine-induced zebrafish depression model was similar to the reserpine mice model and the CUMS mice model in the tyrosine metabolism pathway. The above results showed that the reserpine-induced depression zebrafish model was similar to the CUMS model from phenotype to internal metabolic changes and can replace the CUMS model for antidepressants screening. Moreover, the results from this model were obtained in a short time, which can shorten the cycle of drug screening and achieve high-throughput screening. Therefore, we believe it is a reliable high-throughput screening model.

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Section: Cums Modelmentioning

confidence: 99%

A Reliable High-Throughput Screening Model for Antidepressant

Zhang

Qiao

Liu

et al. 2021

IJMS

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“…For example zebrafish demonstrate high genetic and physiological similarity to humans 34 , 35 , and possess major, evolutionarily conserved neurotransmitter systems 36 , 37 and shared central nervous system (CNS) morphology 38 , 39 . Zebrafish are also widely used in stress research 40 – 42 , often based on various aquatic CUS protocols adapted from rodent models 43 – 47 . Here, we utilize a rigorous 5-week CUS protocol, already established in our laboratory 48 , to examine brain transcriptomic changes and weekly dynamics of behavioral and neurochemical stress responses in adult zebrafish (Fig.…”

Section: Introductionmentioning

confidence: 99%

Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

Demin

Lakstygal

Krotova

et al. 2020

Sci Rep

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Stress-related neuropsychiatric disorders are widespread, debilitating and often treatment-resistant illnesses that represent an urgent unmet biomedical problem. Animal models of these disorders are widely used to study stress pathogenesis. A more recent and historically less utilized model organism, the zebrafish (Danio rerio), is a valuable tool in stress neuroscience research. Utilizing the 5-week chronic unpredictable stress (CUS) model, here we examined brain transcriptomic profiles and complex dynamic behavioral stress responses, as well as neurochemical alterations in adult zebrafish and their correction by chronic antidepressant, fluoxetine, treatment. Overall, CUS induced complex neurochemical and behavioral alterations in zebrafish, including stable anxiety-like behaviors and serotonin metabolism deficits. Chronic fluoxetine (0.1 mg/L for 11 days) rescued most of the observed behavioral and neurochemical responses. Finally, whole-genome brain transcriptomic analyses revealed altered expression of various CNS genes (partially rescued by chronic fluoxetine), including inflammation-, ubiquitin- and arrestin-related genes. Collectively, this supports zebrafish as a valuable translational tool to study stress-related pathogenesis, whose anxiety and serotonergic deficits parallel rodent and clinical studies, and genomic analyses implicate neuroinflammation, structural neuronal remodeling and arrestin/ubiquitin pathways in both stress pathogenesis and its potential therapy.

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“…In a chronic model of unpredictable stress, the protocol increased anxiety-like behavior, impaired memory and induced neuroendocrine dysfunction [5]. Other protocols have extended the understanding of the effects of chronic stress in zebrafish [9][10][11][12]. However, the modulation of stress response by drugs is poorly understood.…”

Section: Introductionmentioning

confidence: 99%