Unraveling Endothelial Cell Migration: Insights into Fundamental Forces, Inflammation, Biomaterial Applications, and Tissue Regeneration Strategies

Jerka, Dominika; Bonowicz, Klaudia; Piekarska, Klaudia; Gokyer, Seyda; Derici, Utku Serhat; Hindy, Osama Ali; Altunay, Baris Burak; Yazgan, Işıl; Steinbrink, Kerstin; Kleszczyński, Konrad; Yilgor, Pinar; Gagat, Maciej

doi:10.1021/acsabm.3c01227

Cited by 3 publications

References 128 publications

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Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization

Leineweber,

Munares,

Leycam

et al. 2024

Preprint

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Holotomograhic microscopy (HTM) has emerged as a non-invasive imaging technique that offers high-resolution, quantitative 3D imaging of biological samples. This study explores the application of HTM in examining endothelial cells (ECs). HTM overcomes the limitations of traditional microscopy methods in capturing the real-time dynamics of ECs by leveraging the refractive index (RI) to map 3D distributions label-free. This work demonstrates the utility of HTM in visualizing key cellular processes during endothelialization, wherein ECs anchor, adhere, migrate, and proliferate. Leveraging the high resolution and quantitative power of HTM, we show that lipid droplets and mitochondria are readily visualized, enabling more comprehensive studies on their respective roles during endothelialization. The study highlights how HTM can uncover novel insights into EC behavior, offering potential applications in medical diagnostics and research, particularly in developing treatments for cardiovascular diseases. This advanced imaging technique not only enhances our understanding of EC biology but also presents a significant step forward in the study of cardiovascular diseases, providing a robust platform for future research and therapeutic development.

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Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization

Leineweber,

Munares,

Leycam

et al. 2024

Preprint

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Improving Methodology of Endothelial Cell Research: Synopsis and Prospects

Shishkova,

Frolov,

Markova

et al. 2024

Kompleks. probl. serdečno-sosud. zabol.

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HighlightsThe creation of a domestic bank of standardized endothelial cell cultures (arterial, venous, microvascular, valvular, and lymphatic lineages) and the development of an import-substituting technological process for working with them is a key task to enable next-generation endothelial physiology studies.The cultural and molecular justification for selecting the best source of autologous endothelial cells with the highest regenerative potential (in particular in context of their proliferative and angiogenic capacities) represents a primary task for endothelial physiology.The research methodology for studying endothelial cell physiology under normal and pathological conditions includes assessing their proliferative and angiogenic properties and molecular profiling. AbstractOne of the primary goals in studying endothelial physiology is the development of a biomedical cell product in the form of autologous endothelial cells with high regenerative potential. These cells would be used to stimulate angiogenesis in ischemic tissues and to coat tissue-engineered constructs (such as vascular grafts) for their endothelialization prior to implantation into the human body. This review examines the key applied aspects of endothelial physiology aimed at achieving these objectives, including the selection of endothelial cell sources for modeling various pathological processes and regenerative medicine, the creation of a bank of standardized endothelial cell lines with various differentiation directions to enhance the effectiveness of this modeling, methods to ensure adhesion, sustained proliferation, and physiological functioning of primary endothelial cells in culture, as well as the methodology for assessing proliferative and angiogenic activity in the context of analyzing endothelial cell resistance to external influences. The discussion emphasizes the need for a comparative analysis of the regenerative properties of microvascular endothelial cells from subcutaneous adipose tissue, colony-forming endothelial cells from peripheral venous blood, and endothelial cells differentiated from induced pluripotent stem cells. Additionally, the current state of import substitution for various components of endothelial cell technology is reviewed, including standardized cell lines, functional protein coatings, and three-dimensional matrices for angiogenesis assessment, culture media for isolation, cultivation, and experimentation with endothelial cells, as well as other reagents for the isolation and subculturing of endothelial cells, culture dishes, and antibodies for flow cytometric and fluorescent microscopic immunophenotyping. The review also analyzes the prospects for import substitution of missing components, such as certain endothelial cell lines, magnetic beads with immobilized antibodies for their isolation, and antibodies conjugated with various fluorophores.

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Dynamics of Endothelial Cell Diversity and Plasticity in Health and Disease

Larionov,

Hammer,

Fiedler

et al. 2024

Cells

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Endothelial cells (ECs) are vital structural units of the cardiovascular system possessing two principal distinctive properties: heterogeneity and plasticity. Endothelial heterogeneity is defined by differences in tissue-specific endothelial phenotypes and their high predisposition to modification along the length of the vascular bed. This aspect of heterogeneity is closely associated with plasticity, the ability of ECs to adapt to environmental cues through the mobilization of genetic, molecular, and structural alterations. The specific endothelial cytoarchitectonics facilitate a quick structural cell reorganization and, furthermore, easy adaptation to the extrinsic and intrinsic environmental stimuli, known as the epigenetic landscape. ECs, as universally distributed and ubiquitous cells of the human body, play a role that extends far beyond their structural function in the cardiovascular system. They play a crucial role in terms of barrier function, cell-to-cell communication, and a myriad of physiological and pathologic processes. These include development, ontogenesis, disease initiation, and progression, as well as growth, regeneration, and repair. Despite substantial progress in the understanding of endothelial cell biology, the role of ECs in healthy conditions and pathologies remains a fascinating area of exploration. This review aims to summarize knowledge and concepts in endothelial biology. It focuses on the development and functional characteristics of endothelial cells in health and pathological conditions, with a particular emphasis on endothelial phenotypic and functional heterogeneity.

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Unraveling Endothelial Cell Migration: Insights into Fundamental Forces, Inflammation, Biomaterial Applications, and Tissue Regeneration Strategies

Cited by 3 publications

References 128 publications

Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization

Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization

Improving Methodology of Endothelial Cell Research: Synopsis and Prospects

Dynamics of Endothelial Cell Diversity and Plasticity in Health and Disease

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