Unraveling lipid/protein interaction in model lipid bilayers by Atomic Force Microscopy

Alessandrini, Andrea; Facci, Paolo

doi:10.1002/jmr.1083

Cited by 40 publications

(29 citation statements)

References 90 publications

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“…The possibility that the mechanical properties of biological membranes can exert a strong influence on the behavior of integral membrane proteins has been proposed since the first evidences of the properties of mechanosensitive channels (Martinac et al, 1987;Phillips et al, 2009). This scenario points to a role of the physical properties of the bilayer, along with its chemical composition at molecular scale, in affecting membrane functions (Marsh, 2008;McIntosh and Simon, 2006;Lee, 2004;Alessandrini and Facci, 2011a). The validity of this concept is nowadays corroborated by many experimental evidences and, as such, it enables a mathematical and quantitative approach to the understanding of the behavior of membrane proteins as affected by the lipid bilayer properties.…”

Section: Introductionmentioning

confidence: 84%

Nanoscale mechanical properties of lipid bilayers and their relevance in biomembrane organization and function

Alessandrini

Facci

2012

Micron

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The mechanical properties of biological systems are emerging as fundamental in determining their functional activity. For example, cells continuously probe their environment by applying forces and, at the same time, are exposed to forces produced by the same environment. Also in biological membranes, the activity of membrane related proteins are affected by the overall mechanical properties of the hosting environment. Traditionally, the mesoscopic mechanical properties of lipid bilayers have been studied by micropipette aspiration techniques. In recent years, the possibility of probing mechanical properties of lipid bilayers at the nanoscale has been promoted by the force spectroscopy potentiality of Atomic Force Microscopes (AFM). By acquiring force-curves on supported lipid bilayers (SLBs) it is possible to probe the mechanical properties on a scale relevant to the interaction between membrane proteins and lipid bilayers and to monitor changes of these properties as a result of a changing environment. Here, we review a series of force spectroscopy experiments performed on SLBs with an emphasis on the functional consequences the measured mechanical properties can have on membrane proteins. We also discuss the force spectroscopy experiments on SLBs in the context of theories developed for dynamic force spectroscopy experiments with the aim to extract the kinetic and energetic description of the process of membrane rupture.

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Section: Introductionmentioning

confidence: 84%

Nanoscale mechanical properties of lipid bilayers and their relevance in biomembrane organization and function

Alessandrini

Facci

2012

Micron

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“…Many proteins are also localized in the boundary region between the two domains boundaries. Th is behavior can be explained by the concept of interfacial adsorption [17,130].…”

Section: Protein Partitioning In Membrane Domainsmentioning

confidence: 99%

“…At the same time, it would be interesting to access both microscopy and force spectroscopy information with a single technique. Among the considered model membrane systems, Supported Lipid Bilayers are prone to be studied by microscopy techniques such as Atomic Force Microscopy that exhibits, besides, force spectroscopy capabilities [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Model Bio‐Membranes Investigated by AFM and AFS: A Suitable Tool to Unravel Lipid Organization and their Interaction with Proteins

Alessandrini¹,

Facci²

2014

Smart Membranes and Sensors

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Th e study of the biological membrane has largely benefi tted from the exploitation of model bilayer systems. Th ese simplifi ed models of the complex biological membrane composed of thousands of diff erent types of molecules allow both to understand basic physical principles underlying the membrane functioning and to test new techniques that will be subsequently applied to biological membranes. Here we concentrate on one of the most used model systems for this kind of investigations: the Supported Lipid Bilayer (SLB). In particular, we analyze the possibilities of investigation off ered by Atomic Force Microscopy and Spectroscopy (AFM/ AFS) on this model system. We discuss the information that these techniques are able to provide on the phase behavior of the lipid bilayers and on the partitioning of membrane proteins relative to the bilayer lateral heterogeneity. We discuss also the possibility to characterize the mechanical properties of lipid bilayers on the nanometer scale lateral resolution.

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“…A so-called “vesicle rupture method” using small vesicles makes it possible to constitute the unilamellar lipid membrane on a solid surface [5,6,7,8,9,10,11]. This technique is based on the electrical attractive force between the solid glass surface and the lipid, generating a supported lipid bilayer (SLB) [12].…”

Section: Introductionmentioning

confidence: 99%

Micrometer-Scale Membrane Transition of Supported Lipid Bilayer Membrane Reconstituted with Cytosol of Dictyostelium discoideum

Takahashi

Toyota

2017

Life

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Background: The transformation of the supported lipid bilayer (SLB) membrane by extracted cytosol from living resources, has recently drawn much attention. It enables us to address the question of whether the purified phospholipid SLB membrane, including lipids related to amoeba locomotion, which was discussed in many previous studies, exhibits membrane deformation in the presence of cytosol extracted from amoeba; Methods: In this report, a method for reconstituting a supported lipid bilayer (SLB) membrane, composed of purified phospholipids and cytosol extracted from Dictyostelium discoideum, is described. This technique is a new reconstitution method combining the artificial constitution of membranes with the reconstitution using animate cytosol (without precise purification at a molecular level), contributing to membrane deformation analysis; Results: The morphology transition of a SLB membrane composed of phosphatidylcholines, after the addition of cytosolic extract, was traced using a confocal laser scanning fluorescence microscope. As a result, pore formation in the SLB membrane was observed and phosphatidylinositides incorporated into the SLB membrane tended to suppress pore formation and expansion; Conclusions: The current findings imply that phosphatidylinositides have the potential to control cytoplasm activity and bind to a phosphoinositide-containing SLB membrane.

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Unraveling lipid/protein interaction in model lipid bilayers by Atomic Force Microscopy

Cited by 40 publications

References 90 publications

Nanoscale mechanical properties of lipid bilayers and their relevance in biomembrane organization and function

Nanoscale mechanical properties of lipid bilayers and their relevance in biomembrane organization and function

Model Bio‐Membranes Investigated by AFM and AFS: A Suitable Tool to Unravel Lipid Organization and their Interaction with Proteins

Micrometer-Scale Membrane Transition of Supported Lipid Bilayer Membrane Reconstituted with Cytosol of Dictyostelium discoideum

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