Unraveling molecular mechanistic differences in liver metabolism between lean and fat lines of Pekin duck (Anas platyrhynchos domestica): A proteomic study

Zheng, Aijuan; Chang, Wenhuan; Hou, Shuisheng; Zhang, Shu; Cai, Huiyi; Chen, Guilan; Lou, Ruiying; Li, Guohua

doi:10.1016/j.jprot.2013.12.021

Cited by 26 publications

(28 citation statements)

References 68 publications

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“…Pekin duck, a world-famous meat duck breed, shows advantageous characteristics such as fast growth, high feed conversion rate, high reproductive rate and strong disease resistance [1]. Pekin ducks account for about 70 % of the annual meat duck production in Asia.…”

Section: Introductionmentioning

confidence: 99%

“…FPD is used mainly as raw material for roast duck because of its high subcutaneous fat percentage. In China and Southeast Asia, roast Pekin duck is a classic example of the utilization of the Pekin duck's excellent subcutaneous fat trait utilization [1]. However, the intensive selection on growth rate has resulted in a decline in meat quality and poor flavor characteristics in meat animals [4].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of carcass and meat quality traits between lean and fat Pekin ducks

Ding

Chen

et al. 2021

Anim Biosci

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According to market demand, meat duck breeding mainly includes 2 breeding directions: lean Pekin duck (LPD) and fat Pekin duck (FPD). The aim of the present study was to compare carcass and meat quality traits between 2 strains, and to provide basic data for guidelines of processing and meat quality improvement. Methods A total of 62 female Pekin ducks (32 LPDs and 30 FPDs) at the age of 42 days were slaughtered. The live body weight and carcass traits were measured and calculated. Physical properties of breast muscle were determined by texture analyzer and muscle fibers were measured by paraffin sections. The content of inosine monophosphate (IMP), intramuscular fat (IMF) and fatty acids composition were measured by high-performance liquid chromatography, Soxhlet extraction method and automated gas chromatography respectively. Results The results showed that the bodyweight of LPDs was higher than that of FPDs. FPDs were significantly higher than LPDs in subcutaneous fat thickness, subcutaneous fat weight, subcutaneous fat percentage, abdominal fat percentage and abdominal fat shear force (P < 0.01). LPDs were significantly higher than FPDs in breast muscle thickness, breast muscle weight, breast muscle rate and breast muscle shear force (P < 0.01). The muscle fiber average area and fiber diameter of LPDs were significantly higher than those of FPDs (P < 0.01). The muscle fiber density of LPDs was significantly lower than that of FPDs (P < 0.01). The intramuscular fat of LPDs in the breast muscle was significantly higher than that in the FPDs A c c e p t e d A r t i c l e (P < 0.01). There was no significant difference between the 2 strains in inosine monophosphate content (P＞0.05). The polyunsaturated fatty acid content of LPDs was significantly higher than that of FPDs (P < 0.01), and FPDs had higher saturated fatty acid and monounsaturated fatty acid levels (P < 0.05). Conclusion Long-term breeding work resulted in vast differences between the two strains Pekin ducks. This study provides a reference for differences between LPD and FPD that manifest as a result of long-term selection.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of carcass and meat quality traits between lean and fat Pekin ducks

Ding

Chen

et al. 2021

Anim Biosci

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“…In chickens, the expression of lipid synthesis and secretion-related genes were analysed in the liver of lean and fat chickens, and only a few genes (stearoyl-CoA desaturase, sterol response element binding factor 1 and hepatocyte nuclear factor 4) were identified to be differentially expressed between the chicken breeds (Bourneuf et al 2006). In ducks, Aijuan et al identified the differential expression of proteins in hepatic tissues in lean and fat Pekin ducks (Zheng et al 2014). Based on their data, no DNL-related proteins were identified, which was similar to our results that indicated less contribution of hepatic DNL in the genetic fat mass difference.…”

Section: Discussionmentioning

confidence: 99%

The contributions of hepatic de novo lipogenesis to the difference in body fat mass of genetically lean and fat duck breeds

Liu

Zhou

et al. 2017

Journal of Applied Animal Research

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The underlying reasons for genetic differences in body fat mass remain unclear. The objective of this study was to investigate the contributions of hepatic de novo lipogenesis (DNL) to the genetic differences in fat mass in ducks. Ducks with distinct genetic backgrounds were selected for the genetically lean and fat animal models. The weights of fat tissue and organs as well as the gene expressions, enzyme activities and concentrations related to hepatic lipogenesis were measured and compared between the two genetically different duck breeds. Although a clear phenotypic difference in extra-liver fat mass between the two genetically different duck breeds was observed, the relative weights of the fat tissue to body weight in the two breeds were similar. There were no clear divergences regarding DNL-related gene expressions (Acc, Dgat2 and Fas), enzyme activities (ELVOL and FAS) and enzyme concentrations (ACC) between the two genetically different duck breeds, suggesting that hepatic DNL may contribute less to the individual fat mass difference in ducks; rather, body fat mass may depend more on the liver size. Our findings may provide a fundamental explanation for individual fat mass differences. ARTICLE HISTORY

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“…The protein spots were excised and digested with 10 ng/μl trypsin (Roche) for MS analysis as previously reported [ 15 ]. The peptides were analyzed by liquid chromatography-chip/electrospray ionization-quadruple time-of-flight/mass spectrometry (QTOF G6520, Agilent Technologies).…”

Section: Methodsmentioning

confidence: 99%

Molecular Differences in Hepatic Metabolism between AA Broiler and Big Bone Chickens: A Proteomic Study

Zheng

Chang

et al. 2016

PLoS ONE

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Identifying the metabolic differences in the livers of modern broilers and local chicken breeds is important for understanding their biological characteristics, and many proteomic changes in their livers are not well characterized. We therefore analyzed the hepatic protein profiles of a commercial breed, Arbor Acres (AA) broilers, and a local dual purpose breed, Big Bone chickens, using two-dimensional electrophoresis combined with liquid chromatography-chip/electrospray ionization-quadruple time-of-flight/mass spectrometry (LC-MS/MS). A total of 145 proteins were identified as having differential abundance in the two breeds at three growth stages. Among them, 49, 63 and 54 belonged to 2, 4, and 6 weeks of age, respectively. The higher abundance proteins in AA broilers were related to the energy production pathways suggesting enhanced energy metabolism and lipid biosynthesis. In contrast, the higher abundance proteins in Big Bone chickens showed enhanced lipid degradation, resulting in a reduction in the abdominal fat percentage. Along with the decrease in fat deposition, flavor substance synthesis in the meat of the Big Bone chickens may be improved by enhanced abundance of proteins involved in glycine metabolism. In addition, the identified proteins in nucleotide metabolism, antioxidants, cell structure, protein folding and transporters may be critically important for immune defense, gene transcription and other biological processes in the two breeds. These results indicate that selection pressure may have shaped the two lines differently resulting in different hepatic metabolic capacities and extensive metabolic differences in the liver. The results from this study may help provide the theoretical basis for chicken breeding.

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Unraveling molecular mechanistic differences in liver metabolism between lean and fat lines of Pekin duck (Anas platyrhynchos domestica): A proteomic study

Cited by 26 publications

References 68 publications

Comparison of carcass and meat quality traits between lean and fat Pekin ducks

Comparison of carcass and meat quality traits between lean and fat Pekin ducks

The contributions of hepatic de novo lipogenesis to the difference in body fat mass of genetically lean and fat duck breeds

Molecular Differences in Hepatic Metabolism between AA Broiler and Big Bone Chickens: A Proteomic Study

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