Unraveling salt‐responsive tissue‐specific metabolic pathways in olive tree

Skodra, Christina; Michailidis, Michail; Dasenaki, Marilena E.; Ganopoulos, Ioannis; Thomaidis, Nikolaos S.; Tanou, Georgia; Molassiotis, Athanassios

doi:10.1111/ppl.13565

Cited by 16 publications

(13 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A major challenge in characterizing plant metabolism under stress is the compartmentalization at the level of cells and organelles. Distinct tissue-specific metabolic responses to abiotic stresses have been characterized in many plant species [ 76 , 216 , 217 ]. In contrast, our knowledge of cell-specific and organelle-specific metabolic responses has been hampered by the limitations of current analytical methods to determine the cellular and subcellular location of metabolites.…”

Section: Challenges and Perspectives In Deciphering Plant Metabolic R...mentioning

confidence: 99%

Reprogramming of Plant Central Metabolism in Response to Abiotic Stresses: A Metabolomics View

2022

IJMS

View full text Add to dashboard Cite

Abiotic stresses rewire plant central metabolism to maintain metabolic and energy homeostasis. Metabolites involved in the plant central metabolic network serve as a hub for regulating carbon and energy metabolism under various stress conditions. In this review, we introduce recent metabolomics techniques used to investigate the dynamics of metabolic responses to abiotic stresses and analyze the trend of publications in this field. We provide an updated overview of the changing patterns in central metabolic pathways related to the metabolic responses to common stresses, including flooding, drought, cold, heat, and salinity. We extensively review the common and unique metabolic changes in central metabolism in response to major abiotic stresses. Finally, we discuss the challenges and some emerging insights in the future application of metabolomics to study plant responses to abiotic stresses.

show abstract

Section: Challenges and Perspectives In Deciphering Plant Metabolic R...mentioning

confidence: 99%

Reprogramming of Plant Central Metabolism in Response to Abiotic Stresses: A Metabolomics View

2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Furthermore, a lower malic acid content was observed in the infected samples compared to the non-infected counterparts. Malic acid is a key metabolite in the biosynthesis of amino acids and fatty acids and is involved in the citric acid cycle [59]. In a previous NMR-based metabolomics study, a decrease in malate content was observed in symptomatic Huanglongbing (HLB) leaf extracts [60].…”

Section: Discussionmentioning

confidence: 99%

Non-Targeted Spectranomics for the Early Detection of Xylella fastidiosa Infection in Asymptomatic Olive Trees, cv. Cellina di Nardò

Ahmed,

Musio,

Todisco

et al. 2023

Molecules

View full text Add to dashboard Cite

Olive quick decline syndrome (OQDS) is a disease that has been seriously affecting olive trees in southern Italy since around 2009. During the disease, caused by Xylella fastidiosa subsp. pauca sequence type ST53 (Xf), the flow of water and nutrients within the trees is significantly compromised. Initially, infected trees may not show any symptoms, making early detection challenging. In this study, young artificially infected plants of the susceptible cultivar Cellina di Nardò were grown in a controlled environment and co-inoculated with additional xylem-inhabiting fungi. Asymptomatic leaves of olive plants at an early stage of infection were collected and analyzed using nuclear magnetic resonance (NMR), hyperspectral reflectance (HSR), and chemometrics. The application of a spectranomic approach contributed to shedding light on the relationship between the presence of specific hydrosoluble metabolites and the optical properties of both asymptomatic Xf-infected and non-infected olive leaves. Significant correlations between wavebands located in the range of 530–560 nm and 1380–1470 nm, and the following metabolites were found to be indicative of Xf infection: malic acid, fructose, sucrose, oleuropein derivatives, and formic acid. This information is the key to the development of HSR-based sensors capable of early detection of Xf infections in olive trees.

show abstract

“…Interestingly, an 8-fold increase of the first enzyme in this pathway DOXP-synthase is detected, while intermediate synthases exclusive to iridoid synthesis, are only 3.5-and 2-fold. The enhanced activation of DOXP-synthase speaks of an activation devoted to feed synthesis of other relevant terpenes with physiological or biological activity, such as ABA, key for stomatal control, or oleacin synthesis ( El and Karakaya, 2009 ; Li et al, 2020 ; Skodra et al, 2021 ; Xie et al, 2021 ). A striking 5-fold increase in the target metabolite oleuropein was found, suggesting either activation of the final step in the pathway, or a strong inhibition of oleuropein-β-glucosidase (OeGLU), the enzyme that would transform oleuropein into its aglycon, which would be further transformed into oleacin by demethylation and decarboxylation ( Koudounas et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, photosynthesis arrest may happen due to many factors including high light intensity, temperature, and water resource availability ( Skodra et al, 2021 ). On one hand, the usual oxidative stress of light reactions is further enhanced under stress and needs to be counteracted by an active ROS scavenging system, allowing ROS within healthy levels for systemic metabolic coordination ( Baxter et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

“…Olea europaea is one of the most extended crops in the Mediterranean area, naturally endowed with mechanisms for high water use efficiency. The most characteristic secondary metabolites present in olive trees are iridoids, triterpenes, and phenolic compounds (flavonols), all conferring a high antioxidant potential ( Khaliq et al, 2015 ; Skodra et al, 2021 ). Biosynthesis of these metabolites involves the DOXP-pathway for iridoids, the mevalonic acid pathway for triterpenes, and the shikimate-flavonol pathway for flavonols, which accumulate in fruits and also in leaves.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacillus H47 triggers Olea europaea metabolism activating DOXP and shikimate pathways simultaneously and modifying leaf extracts’ antihypertensive activity

et al. 2022

View full text Add to dashboard Cite

Improvement of plant adaptation by beneficial bacteria (PGPB) may be achieved by triggering multiple pathways to overcome the environmental stress on plant’s growth cycle, activating plant’s metabolism. The present work reports the differential ability of three Bacillus strains to trigger olive tree metabolism, among which, only H47 was outstanding increasing iridoid and flavonol concentration. One-year old olive seedlings grown open air, under harsh conditions of water shortage in saline soils, were root-inoculated with three Bacillus PGPB strains throughout a 12-month period after which, photosynthesis was determined; photosynthetic pigments and bioactive secondary metabolites (iridoids and flavonols) were analyzed, and a study of gene expression of both pathways involved was undertaken to unravel molecular targets involved in the activation. All three strains increased plant fitness based on photosynthetic values, increasing energy dissipation capacity to lower oxidative stress; only H47 increased CO2 fixation and transpiration. Bacillus H47 was found to trigger synthases in the DOXP pathway (up to 5-fold in DOXP-synthase, 3.5-fold in Iridoid synthase, and 2-fold in secologanin synthase) associated to a concomitant increase in iridoids (up to 5-fold in oleuropein and 2-fold in its precursor secologanin). However, despite the 2-fold increases detected in the two predominant flavonols, gene expression was not enhanced, suggesting involvement of a pulse activation model proposed for innate immunity. Furthermore, the activity of leaf extracts to inhibit Angiotensin Converting Enzyme was evaluated, to explore further uses of plant debris with higher added value. Despite the increases in iridoids, leaf extracts from H47 did not increase ACE inhibition, and still, increased antihypertensive potential in oil obtained with this strain is to be explored, as leaves are the source for these metabolites which further translocate to fruits. In summary, Bacillus H47 is an effective strain to increase plant adaptation to dry and saline environments, activates photosynthesis and secondary metabolism in olive tree.

show abstract

Unraveling salt‐responsive tissue‐specific metabolic pathways in olive tree

Cited by 16 publications

References 62 publications

Reprogramming of Plant Central Metabolism in Response to Abiotic Stresses: A Metabolomics View

Reprogramming of Plant Central Metabolism in Response to Abiotic Stresses: A Metabolomics View

Non-Targeted Spectranomics for the Early Detection of Xylella fastidiosa Infection in Asymptomatic Olive Trees, cv. Cellina di Nardò

Bacillus H47 triggers Olea europaea metabolism activating DOXP and shikimate pathways simultaneously and modifying leaf extracts’ antihypertensive activity

Contact Info

Product

Resources

About