Unraveling the emergence and population diversity of Listeria monocytogenes in a newly built meat facility through whole genome sequencing

Alvarez-Molina, Adrián; Cobo‐Díaz, José F.; López, Mercedes; Prieto, Miguel; Toro, María de; Álvarez‐Ordóñez, Avelino

doi:10.1016/j.ijfoodmicro.2021.109043

Cited by 45 publications

(31 citation statements)

References 83 publications

(93 reference statements)

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“…All but one strain were recovered from RTE food (17; 60.7%) and food production environments (10; 35.7%). L. monocytogenes of ST8, such as the characterized above ST121, was previously often isolated from food and food production areas, which suggests that strains of such sequence type can survive in adverse conditions present in such environments [ 13 , 74 , 77 , 79 , 80 ]. Furthermore, such isolates have probably increased virulence properties since they were also responsible for human listeriosis cases [ 47 , 59 , 81 , 82 ].…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have indicated strains divergence regarding their ability to persist in the environment as well as their virulence potential [ 10 ]. It has been shown that among isolates classified into the evolutionary line II, there are L. monocytogenes of serogroup IIa, which are often over-represented in food and food processing environments; such isolates are usually characterized by a high prevalence of various virulence marker genes and are often identified in humans with listeriosis [ 11 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity and Relationships of Listeria monocytogenes Serogroup IIa Isolated in Poland

2022

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In the present study, 100 L. monocytogenes isolates of serogroup IIa from food and food production environments in Poland were characterized towards the presence of virulence, resistance, and stress response genes using whole-genome sequencing (WGS). The strains were also molecularly typed and compared with multi-locus sequence typing (MLST) and core genome MLST analyses. The present isolates were grouped into 6 sublineages (SLs), with the most prevalent SL155 (33 isolates), SL121 (32 isolates), and SL8 (28 isolates) and classified into six clonal complexes, with the most prevalent CC155 (33 strains), CC121 (32 isolates), and CC8 (28 strains). Furthermore, the strains were grouped to eight sequence types, with the most prevalent ST155 (33 strains), ST121 (30 isolates), and ST8 (28; strains) followed by 60 cgMLST types (CTs). WGS data showed the presence of several virulence genes or putative molecular markers playing a role in pathogenesis of listeriosis and involved in survival of L. monocytogenes in adverse environmental conditions. Some of the present strains were molecularly closely related to L. monocytogenes previously isolated in Poland. The results of the study showed that food and food production environments may be a source of L. monocytogenes of serogroup IIa with pathogenic potential.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Relationships of Listeria monocytogenes Serogroup IIa Isolated in Poland

2022

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“…These assays were performed on plates that had been subjected to 5 sanitization cycles as described above as well as on plates that had not been sanitized with any of the solutions. A three-strain cocktail of L. monocytogenes containing the CECT911 reference strain and two strains previously isolated from the processing environment of a meat industry (ULE1264 and ULE1265) [19] was used. The bacterial strains were stored frozen, and they were revitalized previously to the preparation of the stock cultures.…”

Section: Biofilm Formation Quantificationmentioning

confidence: 99%

Durability Assessment of a Plasma-Polymerized Coating with Anti-Biofilm Activity against L. monocytogenes Subjected to Repeated Sanitization

Muro-Fraguas

Fernández-Gómez

Múgica‐Vidal

et al. 2021

Foods

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Biofilm formation on food-contact surfaces is a matter of major concern causing food safety and spoilage issues to this sector. The aim of this study was to assess the durability of the anti-biofilm capacity of a plasma-polymerized coating composed of a base coating of (3-aminopropyl)triethoxysilane (APTES) and a functional coating of acrylic acid (AcAc). Coated and uncoated AISI 316 stainless steel (SS) plates were subjected to five sanitization cycles with sodium hypochlorite (0.05%) and peracetic acid (0.5%). The effectiveness of the coating for the inhibition of multi-strain Listeria monocytogenes biofilm formation was confirmed using a three-strain cocktail, which was grown on the SS plates at 12 °C for 6 days. Compared to the uncoated SS, relative biofilm productions of 14.6% on the non-sanitized coating, 27.9% on the coating after sanitization with sodium hypochlorite, and 82.3% on the coating after sanitization with peracetic acid were obtained. Morphological and physicochemical characterization of the coatings suggested that the greater anti-biofilm effectiveness after sanitization with sodium hypochlorite was due to the high pH of this solution, which caused a deprotonation of the carboxylic acid groups of the functional coating. This fact conferred it a strong hydrophilicity and negatively charged its surface, which was favorable for preventing bacterial attachment and biofilm formation.

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“…Many produce outbreaks and recalls due to L. monocytogenes are traced back to the processing environment [ 9 ]. The ability of L. monocytogenes to grow and spread at low temperature conditions and its tolerance to stress conditions such as pH and salinity not only contributes to its ubiquitous nature but also to its persistence in food processing environments and high risk of cross-contamination [ 10 , 11 ]. The capability of L. monocytogenes to persist in inaccessible surfaces that are difficult to clean favours the exposure of the bacteria to dilute subinhibitory concentrations of sanitizers [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Frozen Vegetable Processing Plants Can Harbour Diverse Listeria monocytogenes Populations: Identification of Critical Operations by WGS

Truchado

Gil

Querido-Ferreira

et al. 2022

Foods

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Frozen vegetables have emerged as a concern due to their association with foodborne outbreaks such as the multi-country outbreak of Listeria monocytogenes serogroup IVb linked to frozen corn. The capacity of L. monocytogenes to colonize food-processing environments is well-known, making the bacteria a real problem for consumers. However, the significance of the processing environment in the contamination of frozen foods is not well established. This study aimed to identify potential contamination niches of L. monocytogenes in a frozen processing plant and characterize the recovered isolates. A frozen vegetable processing plant was monitored before cleaning activities. A total of 78 points were sampled, including frozen vegetables. Environmental samples belonged to food-contact surfaces (FCS); and non-food-contact surfaces (n-FCS). Positive L. monocytogenes samples were found in FCS (n = 4), n-FCS (n = 9), and the final product (n = 1). A whole-genome sequencing (WGS) analysis revealed two clusters belonging to serotypes 1/2a-3a and 1/2b-3b). The genetic characterization revealed the presence of four different sequence types previously detected in the food industry. The isolate obtained from the final product was the same as one isolate found in n-FCS. A multi-virulence-locus sequence typing (MVLST) analysis showed four different virulence types (VT). The results obtained highlight the relevant role that n-FCS such as floors and drains can play in spreading L. monocytogenes contamination to the final product.

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Unraveling the emergence and population diversity of Listeria monocytogenes in a newly built meat facility through whole genome sequencing

Cited by 45 publications

References 83 publications

Genetic Diversity and Relationships of Listeria monocytogenes Serogroup IIa Isolated in Poland

Genetic Diversity and Relationships of Listeria monocytogenes Serogroup IIa Isolated in Poland

Durability Assessment of a Plasma-Polymerized Coating with Anti-Biofilm Activity against L. monocytogenes Subjected to Repeated Sanitization

Frozen Vegetable Processing Plants Can Harbour Diverse Listeria monocytogenes Populations: Identification of Critical Operations by WGS

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