Unraveling the induction of phytoene synthase 2 expression by salt stress and abscisic acid in Daucus carota

Simpson, Kevin; Fuentes, Paulina; Quiroz-Iturra, Luis Felipe; Flores-Ortiz, Carlos; Contreras, Rodrigo A.; Handford, Michael; Stange, Claudia

doi:10.1093/jxb/ery207

Cited by 56 publications

(42 citation statements)

References 110 publications

(186 reference statements)

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“…Interestingly, exogenous ABA application induced PSY expression in Arabidopsis roots, leading to an increase in carotenoid accumulation (Meier et al ., 2011; Ruiz‐Sola et al ., 2014). Furthermore, a recent study showed that PSY2 expression was induced in carrot plants treated with ABA (Simpson et al , 2018). In addition, characterization of the DcPSY2 promoter revealed three ABA and GA regulatory elements (Simpson et al , 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, a recent study showed that PSY2 expression was induced in carrot plants treated with ABA (Simpson et al , 2018). In addition, characterization of the DcPSY2 promoter revealed three ABA and GA regulatory elements (Simpson et al , 2018). Thus, higher GA and ABA contents in our tobacco lines might be responsible for the increased PSY expression (and probably the expression of other carotenoid genes) and the elevated carotenoid content.…”

Section: Discussionmentioning

confidence: 99%

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Expression of a carotenogenic gene allows faster biomass production by redesigning plant architecture and improving photosynthetic efficiency in tobacco

Moreno

Agrawal

et al. 2020

The Plant Journal

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SUMMARY Because carotenoids act as accessory pigments in photosynthesis, play a key photoprotective role and are of major nutritional importance, carotenogenesis has been a target for crop improvement. Although carotenoids are important precursors of phytohormones, previous genetic manipulations reported little if any effects on biomass production and plant development, but resulted in specific modifications in carotenoid content. Unexpectedly, the expression of the carrot lycopene β‐cyclase (DcLCYB1) in Nicotiana tabacum cv. Xanthi not only resulted in increased carotenoid accumulation, but also in altered plant architecture characterized by longer internodes, faster plant growth, early flowering and increased biomass. Here, we have challenged these transformants with a range of growth conditions to determine the robustness of their phenotype and analyze the underlying mechanisms. Transgenic DcLCYB1 lines showed increased transcript levels of key genes involved in carotenoid, chlorophyll, gibberellin (GA) and abscisic acid (ABA) biosynthesis, but also in photosynthesis‐related genes. Accordingly, their carotenoid, chlorophyll, ABA and GA contents were increased. Hormone application and inhibitor experiments confirmed the key role of altered GA/ABA contents in the growth phenotype. Because the longer internodes reduce shading of mature leaves, induction of leaf senescence was delayed, and mature leaves maintained a high photosynthetic capacity. This increased total plant assimilation, as reflected in higher plant yields under both fully controlled constant and fluctuating light, and in non‐controlled conditions. Furthermore, our data are a warning that engineering of isoprenoid metabolism can cause complex changes in phytohormone homeostasis and therefore plant development, which have not been sufficiently considered in previous studies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression of a carotenogenic gene allows faster biomass production by redesigning plant architecture and improving photosynthetic efficiency in tobacco

Moreno

Agrawal

et al. 2020

The Plant Journal

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“…Huang et al found that genes involved in the biosynthesis and signal pathway of ABA may be affected by carotenogenesis in carrot 137 . The expression level of DcPSY2 (phytoene synthase) can be induced by salt stress and ABA 138 . In Arabidopsis , salt stress was found to induce carotenoid synthesis to contribute to ABA production 139 .…”

Section: Hormone Regulationmentioning

confidence: 99%

Advances in research on the carrot, an important root vegetable in the Apiaceae family

et al. 2019

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Carrots ( Daucus carota L.), among the most important root vegetables in the Apiaceae family, are cultivated worldwide. The storage root is widely utilized due to its richness in carotenoids, anthocyanins, dietary fiber, vitamins and other nutrients. Carrot extracts, which serve as sources of antioxidants, have important functions in preventing many diseases. The biosynthesis, metabolism, and medicinal properties of carotenoids in carrots have been widely studied. Research on hormone regulation in the growth and development of carrots has also been widely performed. Recently, with the development of high-throughput sequencing technology, many efficient tools have been adopted in carrot research. A large amount of sequence data has been produced and applied to improve carrot breeding. A genome editing system based on CRISPR/Cas9 was also constructed for carrot research. In this review, we will briefly summarize the origins, genetic breeding, resistance breeding, genome editing, omics research, hormone regulation, and nutritional composition of carrots. Perspectives about future research work on carrots are also briefly provided.

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“…The purpose of this study was not to examine abiotic stress tolerance but several studies have examined biochemical and molecular mechanisms underlying tolerance in carrot. Phytoene synthase is a key enzyme in the biosynthesis of carotenoid pigments critical for photosynthesis and its biosynthesis in carrot is upregulated under salinity stress (Fuentes et al., 2012; Simpson et al., 2018). A carrot heat shock protein contributing to heat tolerance was described in carrot (Malik, Slovin, Hwang, & Zimmerman, 1999) and several other carrot heat shock proteins have recently been described (Huang & Xu, 2008; Huang et al., 2015).…”

Section: Resultsmentioning

confidence: 99%

Wild carrot diversity for new sources of abiotic stress tolerance to strengthen vegetable breeding in Bangladesh and Pakistan

et al. 2020

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Crop wild relatives (CWRs) of carrot (Daucus. carota L.) including 64 germplasm accessions of D. carota L. subsp. carota, D. carota L. subsp. capillifolius (Gilli) Arbizu, and D. carota L. subsp. gummifer (Syme) Hook. f., as well as two accessions of another 18‐chromosome species, D. syrticus Murb., were grown in field trials to flowering under conditions of heat, drought, and salinity stress in Bangladesh and Pakistan. Plant growth before floral initiation was evaluated and plants were allowed to progress to flowering to evaluate seed production. A wide range of response was observed among entries ranging from no germination or plant death early in development to vigorous growth. Carrot grown under optimal conditions in the greenhouse served as a useful control to estimate tolerance indices in the field. New sources of heat and drought tolerance in subsp. carota and subsp. capillifolius accessions were identified with similar performance in both countries. Ecogeographic analysis demonstrated that environmental parameters at the collection location of germplasm were associated with heat and drought tolerance observed in field trials. Plants evaluated for tolerance were allowed to flower and seed was produced on selected plants with abiotic stress tolerance to develop populations or breeding pools and to initiate the development of carrot better adapted to climatic abiotic stress.

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Unraveling the induction of phytoene synthase 2 expression by salt stress and abscisic acid in Daucus carota

Abstract: ABA increased during salt stress triggers binding of AREB/ABFs to DcPSY2 promoter, boosting carotenoid and ABA levels as a positive feedback mechanism

Cited by 56 publications

References 110 publications

Expression of a carotenogenic gene allows faster biomass production by redesigning plant architecture and improving photosynthetic efficiency in tobacco

Expression of a carotenogenic gene allows faster biomass production by redesigning plant architecture and improving photosynthetic efficiency in tobacco

Advances in research on the carrot, an important root vegetable in the Apiaceae family

Wild carrot diversity for new sources of abiotic stress tolerance to strengthen vegetable breeding in Bangladesh and Pakistan

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