Unraveling the mechanism of neuroprotection of curcumin in arsenic induced cholinergic dysfunctions in rats

Srivastava, Pranay; Yadav, Rajesh Singh; Chandravanshi, Lalit P.; Shukla, Rohit; Dhuriya, Yogesh K.; Chauhan, Lalit Kumar Singh; Dwivedi, Hari N.; Pant, Aditya B.; Khanna, Vinay K.

doi:10.1016/j.taap.2014.06.006

Cited by 64 publications

(43 citation statements)

References 110 publications

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“…Arsenic exposure is associated with considerable human morbidity and mortality. There is accumulating evidence that chronic exposure to arsenic, even at fairly low levels, leads to increased risk of infectious diseases, such as polyneuritis, diarrhoea, and immunosuppression of the T-dependent antibody response [2, 3]. Moreover, arsenic exposure in individuals promotes the generation of reactive oxygen species and nitric oxide and is involved in the production of pro-inflammatory cytokines [4-6].…”

Section: Introductionmentioning

confidence: 99%

“…These factors deteriorate in arsenic-related dysfunction of the immune system, particular immunosuppression [7]. Additionally, such alterations of these factors in intracellular and intercellular homeostasis probably lead to cell death and mitochondrial dysfunction, thereby strengthening the activation of stress-related genes [3, 8]. Meanwhile, arsenic exposure through contaminated food materials is quite common in areas with high levels of arsenic in the groundwater, which is becoming an urgent issue in the field of public health.…”

Section: Introductionmentioning

confidence: 99%

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(-)-Epigallocatechin-3-Gallate Inhibits Arsenic-Induced Inflammation and Apoptosis through Suppression of Oxidative Stress in Mice

Yu¹,

Pei

Huang³

et al. 2017

Cell Physiol Biochem

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Background/Aims: Exposure to arsenic in individuals has been found to be associated with various health-related problems including skin lesions, cancer, and cardiovascular and immunological disorders. (-)-Epigallocatechin-3-gallate (EGCG), the main and active polyphenolic catechin present in green tea, has shown potent antioxidant, anti-apoptotic and anti-inflammatory activity in vivo and in vitro. Thus, the present study was conducted to investigate the protective effects of EGCG against arsenic-induced inflammation and immunotoxicity in mice. Methods: Serum IL-1β, IL-6 and TNF-α were determined by ELISA, tissue catalase (CAT), malonyldialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), nitric oxide and caspase 3 by commercial kits, mitochondrial membrane potential with Rh 123, mitochondrial ROS with 2’,7’-dichlorofluorescin diacetate (DCFH-DA), apoptotic and necrotic cells and T-cell phenotyping with Flow cytometry analysis. Results: The results showed that arsenic treatment significantly increased oxidative stress levels (as indicated by catalase, malonyldialdehyde, superoxide dismutase, glutathione and reactive oxygen species), increased levels of inflammatory cytokines and promoted apoptosis. Arsenic exposure increased the relative frequency of the CD8+(Tc) cell subpopulation (from 2.8 to 18.9%) and decreased the frequency of CD4+(Th) cells (from 5.2 to 2.7%). Arsenic exposure also significantly decreased the frequency of T(CD3) (from 32.5% to 19.2%) and B(CD19) cells (from 55.1 to 32.5%). All of these effects induced by NaAsO₂ were attenuated by EGCG. Conclusions: The present in vitro findings indicate that EGCG attenuates not only NaAsO₂-induced immunosuppression but also inflammation and apoptosis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

(-)-Epigallocatechin-3-Gallate Inhibits Arsenic-Induced Inflammation and Apoptosis through Suppression of Oxidative Stress in Mice

Yu¹,

Pei

Huang³

et al. 2017

Cell Physiol Biochem

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“…With technological advancements and efficient imaging techniques have revolutionized the concept of 3D stem cell based organoid research. Pharmacological intervention utilizing natural agents like curcumin has shown neuroprotective efficacy in clinical and experimental models of neurotoxicity and can provide beneficial effects in the neurodegenerative disorders in future (Srivastava et al, 2014). However, the current prevalent pharmacological treatments provide symptomatic relief only for a limited period of time and the drugs administered may have side effects.…”

Section: Discussionmentioning

confidence: 99%

Advances in Stem Cell Research- A Ray of Hope in Better Diagnosis and Prognosis in Neurodegenerative Diseases

Singh

Srivastav

Srivastava

et al. 2016

Front. Mol. Biosci.

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Neurodegeneration and neurodegenerative disorders have been a global health issue affecting the aging population worldwide. Recent advances in stem cell biology have changed the current face of neurodegenerative disease modeling, diagnosis, and transplantation therapeutics. Stem cells also serve the purpose of a simple in-vitro tool for screening therapeutic drugs and chemicals. We present the application of stem cells and induced pluripotent stem cells (iPSCs) in the field of neurodegeneration and address the issues of diagnosis, modeling, and therapeutic transplantation strategies for the most prevalent neurodegenerative disorders. We have discussed the progress made in the last decade and have largely focused on the various applications of stem cells in the neurodegenerative research arena.

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“…Environmental exposure to the inorganic arsenic compound is a global health concern for human and animal, especially in countries such as Bangladesh, India, and Pakistan [1, 2]. Drinking water and industrial effluence are the leading sources of human exposure to inorganic arsenic [3].…”

Section: Introductionmentioning

confidence: 99%

In Vivo and In Vitro Hepatoprotective Effects of Geranium koreanum Methanolic Extract via Downregulation of MAPK/Caspase‐3 Pathway

Akanda

Kim

Ahn

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Geranium koreanum (GK) is an indigenous Chinese herbal medicine widely used for the treatment of various inflammation and liver disorders. However, the exact mechanism of action of GK remains unknown. This study aimed to investigate the protective effect and related molecular mechanism of GK on NaAsO2-induced cytotoxicity in HepG2 cells and liver damage in mice. The cytoprotective role of GK was assessed on HepG2 cells using MTT assay. Oxidative stress and lactate dehydrogenase levels were measured with ROS and LDH assay. Histopathology and serum enzymes levels were estimated. The molecular mechanism was evaluated by qPCR and immunoblotting to ensure the hepatoprotective role of GK against NaAsO2 intoxication in mice. We found cotreatment with GK significantly attenuated NaAsO2-induced cell viability loss, intracellular ROS, and LDH release. Hepatic histopathology and serum biochemical parameters, ALT, and AST were notably improved by cotreatment with GK. Beside, GK markedly altered both mRNA and protein expression level of MAPK. The proapoptotic and antiapoptotic protein Bax/Bcl-2 ratio was significantly regulated by GK. Moreover, GK remarkably suppressed the postapoptotic transcription protein cleaved caspase-3 expression. The present study reveals that GK possesses hepatoprotective activity which is probably involved in the modulation of the MAPK/caspase-3 pathway.

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Unraveling the mechanism of neuroprotection of curcumin in arsenic induced cholinergic dysfunctions in rats

Cited by 64 publications

References 110 publications

(-)-Epigallocatechin-3-Gallate Inhibits Arsenic-Induced Inflammation and Apoptosis through Suppression of Oxidative Stress in Mice

(-)-Epigallocatechin-3-Gallate Inhibits Arsenic-Induced Inflammation and Apoptosis through Suppression of Oxidative Stress in Mice

Advances in Stem Cell Research- A Ray of Hope in Better Diagnosis and Prognosis in Neurodegenerative Diseases

In Vivo and In Vitro Hepatoprotective Effects of Geranium koreanum Methanolic Extract via Downregulation of MAPK/Caspase‐3 Pathway

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