Unraveling the Optimal Culture Condition for the Antifungal Activity and IAA Production of Phylloplane Serratia plymuthica

Aisyah, Siti; Maldoni, Jefri; Sulastri, Irma; Weni, Suryati; Marlisa, Yuli; Herliana, Lissa; Syukriani, Lily; Renfiyeni, Renfiyeni; Jamsari, Jamsari

doi:10.3923/ppj.2019.31.38

Cited by 8 publications

(5 citation statements)

References 19 publications

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“…The stationary phase on the LB medium (Fig. 1a) was faster than Aisyah et al 5 , at 16 hrs of culture duration. The growth curves on the YM medium with tryptophan showed unstable cell numbers after the initial stationary phase.…”

Section: Discussionmentioning

confidence: 62%

“…PGPB with IAA producing ability need specific time incubation to maximize the production. In this study, we used Serratia plymuthica UBCF̲13 as IAA-producing-PGPB and generated the growth curve in LB and YM as the best media to produce IAA 5 . The stationary phase on the LB medium (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…IAA is a secondary metabolite produced in the stationary phase in which the condition of the culture medium has lacked nutrients 27 . A previous study showed the optimal duration of UBCF̲13 to produce IAA was 48 hrs 5 . The differences in the media composition may affect the time at which bacteria enter the stationary phase, thereby affecting the optimal time for IAA production.…”

Section: Discussionmentioning

confidence: 99%

“…UBCF̲13 is a strain of the Biotechnology Laboratory, Faculty of Agriculture, Universitas Andalas Collection which was isolated from Brassica juncea L. leaves 4 . The IAA production from this strain is known to have a positive effect on the growth of chilli 5 . Optimization of IAA production from UBCF̲13 was investigated for the first time in luria bertani (LB) medium and optimal production was gained with the addition of 200 µg mLG 1 tryptophan at 48 hrs of duration culture 5 .…”

Section: Introductionmentioning

confidence: 98%

“…The IAA production from this strain is known to have a positive effect on the growth of chilli 5 . Optimization of IAA production from UBCF̲13 was investigated for the first time in luria bertani (LB) medium and optimal production was gained with the addition of 200 µg mLG 1 tryptophan at 48 hrs of duration culture 5 . The next optimization of culture media for IAA production was found in the yeast mannitol (YM) medium with the addition of 300 µg mLG 1 tryptophan but the optimal culture duration for IAA production in the YM medium needs to be evaluated.…”

Section: Introductionmentioning

confidence: 98%

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Growth Phase Influence the Gene Expression and Metabolite Production Related to Indole-3-Acetic Acid (IAA) Biosynthesis by Serratia plymuthica UBCF_13

Yusfi¹,

Tjong²,

Chaniago³

et al. 2022

Pakistan J. of Biological Sciences

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Background and Objective: The optimization of the indole-3-acetic acid (IAA) producing capability of Serratia plymuthica UBCF̲13 has been intensively studied. This work tried to reveal the effect of growth phases on IAA production, gene expression and metabolite synthesis related to the IAA biosynthesis pathway. Materials and Methods: The growth curve and IAA production were measured every 3 hrs. The putative IAA biosynthesis pathway was investigated based on the UBCF̲13 genome. To identify the possible pathway of IAA biosynthesis in UBCF̲13, we applied the Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) and High-Performance Liquid Chromatography (HPLC) analysis to measure the transcript levels of each gene and indole metabolite production based on tryptophan treatment at different times of incubation. Results: The optimal IAA production on colorimetric assay was at 9 hrs of incubation (initial stationary phase). The level expression of puuC, DDC, oxdA, amiE, nthA and nthB have been upregulated maximum in 3 hrs of culture time (lag phase), except tyrB and ipdC. The highest transcript level of the genes was found in nitrile hydratase genes (nthA and nthB) and indole-3-acetamide (IAM) has been detected as the only intermediate in the crude extract of UBCF̲13 thus the IAM pathway may be used to produce IAA. The maximum IAA production on HPLC analysis was found at 21 hrs of incubation (late stationary phase). Conclusion: This study gives a new insight that the best time to measure gene expression and intermediates related to the IAA biosynthetic pathway in bacteria was found at a specific growth phase.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%