Unraveling the protective effect of a Drosophila phosphatidylethanolamine-binding protein upon bacterial infection by means of proteomics

Reumer, Ank; Bogaerts, Annelies; Loy, Tom Van; Husson, Steven; Temmerman, Liesbet; Choi, Chingman; Clynen, Elke; Hassan, Bassem A.; Schoofs, Liliane

doi:10.1016/j.dci.2009.06.010

Cited by 25 publications

(20 citation statements)

References 66 publications

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“…This protein is a peptidoglycan recognition protein and key for the initial recognition reaction of pathogens, resulting in the activation of the Toll pathway 57,99 . One reason for the observed lower abundance could be that the pathogen is able to suppress host recognition in order to establish an infection 70 , which would be consistent with previous research 74,88,95,99 . Fungal pathogens have also been described to activate the Prophenol-oxidase (proPO) cascade, and we indeed identified a number of proteins involved in this immune response leading to melanisation and subsequent encapsulation of the invading organism.…”

Section: Discussionsupporting

confidence: 86%

Infections with the Sexually Transmitted Pathogen Nosema apis Trigger an Immune Response in the Seminal Fluid of Honey Bees (Apis mellifera)

et al. 2016

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Honey bee (Apis mellifera) males are highly susceptible to infections with the sexually transmitted fungal pathogen Nosema apis. However, they are able to suppress this parasite in the ejaculate using immune molecules in the seminal fluid. We predicted that males respond to infections by altering the seminal fluid proteome to minimize the risk to sexually transmit the parasite to the queen and her colony. We used iTRAQ isotopic labeling to compare seminal fluid proteins from infected and noninfected males and found that N. apis infections resulted in significant abundance changes in 111 of the 260 seminal fluid proteins quantitated. The largest group of proteins with significantly changed abundances consisted of 15 proteins with well-known immune-related functions, which included two significantly more abundant chitinases in the seminal fluid of infected males. Chitinases were previously hypothesized to be involved in honey bee antifungal activity against N. apis. Here we show that infection with N. apis triggers a highly specific immune response in the seminal fluid of honey bee males.

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Section: Discussionsupporting

confidence: 86%

Infections with the Sexually Transmitted Pathogen Nosema apis Trigger an Immune Response in the Seminal Fluid of Honey Bees (Apis mellifera)

et al. 2016

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“…Finally, although no predicted associations were found for phosphatidylethanolamine-binding protein (AAEL011268), this protein was shown to be associated with a protective effect against bacterial infection in Drosophila [32]. …”

Section: Resultsmentioning

confidence: 99%

Protein expression in the midgut of sugar-fed Aedes albopictus females

et al. 2012

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BackgroundAedes albopictus is a vector for several fatal arboviruses in tropical and sub-tropical regions of the world. The midgut of the mosquito is the first barrier that pathogens must overcome to establish infection and represents one of the main immunologically active sites of the insect. Nevertheless, little is known about the proteins involved in the defense against pathogens, and even in the processing of food, and the detoxification of metabolites. The identification of proteins exclusively expressed in the midgut is the first step in understanding the complex physiology of this tissue and can provide insight into the mechanisms of pathogen-vector interaction. However, identification of the locally expressed proteins presents a challenge because the Ae. albopictus genome has not been sequenced.MethodsIn this study, two-dimensional electrophoresis (2DE) was combined with liquid chromatography in line with tandem mass spectrometry (LC-MS/MS) and data mining to identify the major proteins in the midgut of sugar-fed Ae. albopictus females.ResultsFifty-six proteins were identified by sequence similarity to entries from the Ae. aegypti genome. In addition, two hypothetical proteins were experimentally confirmed. According to the gene ontology analysis, the identified proteins were classified into 16 clusters of biological processes. Use of the STRING database to investigate protein functional associations revealed five functional networks among the identified proteins, including a network for carbohydrate and amino acid metabolism, a group associated with ATP production and a network of proteins that interact during detoxification of toxic free radicals, among others. This analysis allowed the assignment of a potential role for proteins with unknown function based on their functional association with other characterized proteins.ConclusionOur findings represent the first proteome map of the Ae. albopictus midgut and denotes the first steps towards the description of a comprehensive proteome map of this vector. In addition, the data contributes to the functional annotation of Aedes spp. genomes using mass spectrometry-based proteomics data combined with complementary gene prediction methods.

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“…Drosophila PEBP homologs, of which at least seven isoforms are known, are expressed in olfactory cells (Rautureau et al, 2009). Interestingly, PEBP1 overexpression has been shown to protect Drosophila against bacterial infection by enhancing the release of immunity‐related proteins in their hemolymph (Reumer et al, 2009), and in plants, RKIP was found to be associated with growth and differentiation, transforming plants from the vegetative to reproductive growth phases (Banfield and Brady, 2000). Furthermore, RKIP is reportedly involved in Cep290‐mediated photoreceptor degeneration and retinopathy.…”

Section: Flashcard Summarizing the Genetic And Protein Information Fomentioning

confidence: 99%

RKIP: Much more than Raf Kinase inhibitory protein

Al-Mulla

Bitar

Taqi

et al. 2013

Journal Cellular Physiology

115

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From its discovery as a phosphatidylethanolamine-binding protein in bovine brain to its designation as a physiological inhibitor of Raf kinase protein, RKIP has emerged as a critical molecule for maintaining subdued, well-orchestrated cellular responses to stimuli. The disruption of RKIP in a wide range of pathologies, including cancer, Alzheimer's disease, and pancreatitis, makes it an exciting target for individualized therapy and disease-specific interventions. This review attempts to highlight recent advances in the RKIP field underscoring its potential role as a master modulator of many pivotal intracellular signaling cascades that control cellular growth, motility, apoptosis, genomic integrity, and therapeutic resistance. Specific biological and functional niches are highlighted to focus future research towards an enhanced understanding of the multiple roles of RKIP in health and disease.

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Unraveling the protective effect of a Drosophila phosphatidylethanolamine-binding protein upon bacterial infection by means of proteomics

Cited by 25 publications

References 66 publications

Infections with the Sexually Transmitted Pathogen Nosema apis Trigger an Immune Response in the Seminal Fluid of Honey Bees (Apis mellifera)

Infections with the Sexually Transmitted Pathogen Nosema apis Trigger an Immune Response in the Seminal Fluid of Honey Bees (Apis mellifera)

Protein expression in the midgut of sugar-fed Aedes albopictus females

RKIP: Much more than Raf Kinase inhibitory protein

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