Unraveling the role of membrane proteins Notch, Pvr, and EGFR in altering integrin diffusion and clustering

Arora, Neha; Mainali, Dipak; Smith, Emily A.

doi:10.1007/s00216-012-6362-3

Cited by 10 publications

(3 citation statements)

References 44 publications

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“…The outcome of the activation of this signaling depends on the balance between these two pathways, which is associated with the stiffness of the ECM environment (Park et al, 2018). These results support the conclusion that the interaction of ECM molecules with integrin receptors has an influence on the maintenance and development of NSCs (Campos et al, 2006;Alam et al, 2007;Rallis et al, 2010;Arora et al, 2012;Brizzi et al, 2012).…”

Section: Neural Stem Cell Fate Depends On Extracellular Matrix Compossupporting

confidence: 69%

Hydrogels Derivatized With Cationic Moieties or Functional Peptides as Efficient Supports for Neural Stem Cells

et al. 2020

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The increasing incidence of neurodegenerative diseases such as Alzheimer's or Parkinson's disease represents a significant burden for patients and national health systems. The conditions are primarily caused by the death of neurons and other neural cell types. One important aim of current stem cell research is to find a way to replace the lost cells. In this perspective, neural stem cells (NSCs) have been considered as a promising tool in the field of regenerative medicine. The behavior of NSCs is modulated by environmental influences, for example hormones, growth factors, cytokines, and extracellular matrix molecules or biomechanics. These factors can be studied by using well-defined hydrogels, which are polymeric networks of synthetic or natural origin with the ability to swell in water. These gels can be modified with a variety of molecules and optimized with regard to their mechanical properties to mimic the natural extracellular environment. In particular modifications applying distinct units such as functional domains and peptides can modulate the development of NSCs with regard to proliferation, differentiation and migration. One well-known peptide sequence that affects the behavior of NSCs is the integrin recognition sequence RGD that has originally been derived from fibronectin. In the present review we provide an overview concerning the applications of modified hydrogels with an emphasis on synthetic hydrogels based on poly(acrylamides), as modified with either cationic moieties or the peptide sequence RGD. This knowledge might be used in tissue engineering and regenerative medicine for the therapy of spinal cord injuries, neurodegenerative diseases and traumata.

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Section: Neural Stem Cell Fate Depends On Extracellular Matrix Compossupporting

confidence: 69%

Hydrogels Derivatized With Cationic Moieties or Functional Peptides as Efficient Supports for Neural Stem Cells

et al. 2020

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“…The elimination of ILK from NSCs activates the JNK-kinase and stimulates proliferation (Porcheri et al, 2014). Overall, integrin-related signaling can interact with several other signal transduction pathways, such as growth factor-related mechanisms, or the neurogenic Notch-and Wnt-related cascades (Alam et al, 2007;Arora et al, 2012;Brizzi et al, 2012;Campos et al, 2006;Rallis et al, 2010).…”

Section: Integrin Receptor Signaling In Nscs and Glial Progenitorsmentioning

confidence: 99%

The extracellular matrix compartment of neural stem and glial progenitor cells

Faissner

Reinhard

2015

Glia

103

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Neuroepithelial and radial GLIA stem cells generate the majority of the cellular constituents of the central nervous system. Following precisely timed phases of neurogenesis and gliogenesis the stem cells recede, with the exception of adult neural stem cells that persist in two generally accepted canonical neurogenic regions, the subventricular zone of the lateral ventricle and the subgranular zone in the dentate gyrus of the hippocampus. It is believed that adult stem cells reside in privileged stem cell niche environments that provide favorable conditions for self-renewal and maintenance of this cellular compartment. Factors such as morphogens, cytokines, and growth factors influence the developmental pathway of neural stem/progenitor cells. By comparison, less is known about the regulatory roles of glycoproteins and proteoglycans of the extracellular matrix (ECM) and their receptors, although they represent important constituents of the micromolecular environment of the niche. Here, we summarize studies that indicate pivotal roles of the ECM micromilieu for the biology and instrumental use of glial stem and progenitor cells of the CNS. Advancing our understanding of structure-function relationships, signaling motifs and complementary receptors and their signal transduction pathways will be of central importance for the application of these cell types in regenerative medicine.

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“…We take the process to occur instantaneously. For example, the diffusion constant of GPI-APs are ∼ 2.5µm 2 /sec [18] whereas the diffusion constant of off-state integrin is approximately ∼ 0.3µm 2 /sec [19]. Furthermore, a typical size of an aster is 30 − 450 nm, and typical on-time of the integrin is 3 to 33 sec, therefore we consider that the timescale of GPI-APs movement on the PM is much faster compared to the integrin switch times and the lifetime of the associated asters.…”

Section: Introductionmentioning

confidence: 99%

Clustering of lipids driven by integrin

Singha¹,

Polley²,

Barma³

2022

Preprint

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Integrin is an important transmembrane receptor protein which remodels the actin network and anchors the cell membrane towards the extracellular matrix via mechanochemical pathways. The clustering of specific lipids and lipid-anchored proteins, which is essential for a certain type of endocytosis process, is facilitated at integrin-mediated active regions. To study this, we propose a minimal exactly solvable model which includes the interplay of stochastic shuttling between integrin on and off states with the intrinsic dynamics of the membrane. We obtain an analytic expression for the deformation and local membrane velocity, and thereby the evolution of clustering mediated by a single integrin. The deformation, velocity and lipid clustering evolve nonmonotonically and their dependences on the stochastic shuttling timescales and membrane properties are elucidated.

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Unraveling the role of membrane proteins Notch, Pvr, and EGFR in altering integrin diffusion and clustering

Cited by 10 publications

References 44 publications

Hydrogels Derivatized With Cationic Moieties or Functional Peptides as Efficient Supports for Neural Stem Cells

Hydrogels Derivatized With Cationic Moieties or Functional Peptides as Efficient Supports for Neural Stem Cells

The extracellular matrix compartment of neural stem and glial progenitor cells

Clustering of lipids driven by integrin

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