Unraveling the transcriptional features and gene expression networks of pathogenic and saprotrophic
            <i>Ophiostoma</i>
            species during the infection of
            <i>Ulmus americana</i>

de Oliveira, Thais C.; Freyria, Nastasia J.; Sarmiento-Villamil, Jorge Luis; Porth, Ilga; Tanguay, Philippe; Bernier, Louis

doi:10.1128/spectrum.03694-23

Cited by 2 publications

References 99 publications

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Distinct biogeographic patterns for bacteria and fungi in association with Bursaphelenchus xylophilus nematodes and infested pinewood

Cao,

Yang,

et al. 2024

Microbiol Spectr

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Pinewood nematodes (PWN, Bursaphelenchus xylophilus ) are destructive plant parasitic nematodes that cause pine wilt disease (PWD) by attacking the vascular systems of pine trees, resulting in widespread tree mortality. Research has shown that there are connections between nematode-associated microbes and PWD. Yet the variations in microbial communities across different geographic regions are not well-understood. In this study, we examined the bacterial and fungal communities associated with nematodes and infested wood collected from 34 sites across three vegetation zones in China, as well as samples from the United States, using 16S rRNA and internal transcribed spacer (ITS) gene amplicon sequencing. The predominant genera Pseudomonas and Rhodococcus were found in nematodes, and Acinetobacter was present in the wood of PWD-infected pine trees across China. Network analysis revealed that core bacterial taxa belonged to the Pseudomonadota and Actinomycetota phyla for the nematodes, whereas the Pseudomonadota and Bacteroidota phyla were dominant in the infested wood. Identification of enriched key microbial taxa in nematodes and infested wood across vegetation zones indicates distinct biogeographic microbial community structures and key bacterial species. Although the nematode-associated bacterial community showed consistency across geographic distances, the similarity of the PWD pine trees’ bacterial community decreased with distance, suggesting a spatial correlation with environmental variables. Our findings enhance our understanding of the microbiota associated with pinewood nematode (PWN) and offer valuable insights into PWD management. IMPORTANCE Our research uncovered specific bacteria and fungi linked to pinewood nematode (PWN) and infested wood in three different vegetation zones in China, as well as samples from the United States. This sheds light on the critical roles of certain microbial groups, such as Pseudomonas, Acinetobacter , and Stenotrophomonas , in influencing PWN fitness. Understanding these patterns provides valuable insights into the dynamics of PWN-associated microbiomes, offering potential strategies for managing pine wilt disease (PWD). We found significant correlations between geographic distance and similarity in bacterial communities in the infested wood, indicating a spatial influence on wood-associated microbial communities due to limited dispersal and localized environmental conditions. Further investigations of these spatial patterns and driving forces are crucial for understanding the ecological processes that shape microbial communities in complex ecosystems and, ultimately, for mitigating the transmission of PWN in forests.

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Distinct biogeographic patterns for bacteria and fungi in association with Bursaphelenchus xylophilus nematodes and infested pinewood

Cao,

Yang,

et al. 2024

Microbiol Spectr

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Chromosome-Level Genome Assembly Unveils the Molecular Mechanisms Underlying Disease Resistance in Ulmus parvifolia

Lyu,

Sun,

Yan

et al. 2024

Preprint

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The absence of a comprehensive genome assembly for Ulmus parvifolia hinders advancements in scientific research and practical breeding efforts, ultimately affecting the cultivation of elm varieties with enhanced resistance to diseases. In this study, we presented a high-quality chromosome-level genome assembly of U. parvifolia by integrating various sequencing approaches. We discovered that the U. parvifolia genome is more than twice the size of Ulmus americana, primarily due to the large-scale amplification of long terminal repeat (LTR) retrotransposons. Phylogenetic analysis positioned U. parvifolia in a closer evolutionary relationship with Moraceae, followed by Cannabaceae, Rhamnaceae, and Rosaceae. Notably, gene families associated with disease resistance and immune response were significantly expanded in U. parvifolia, pointing to an adaptive evolution to various biotic and abiotic stresses. Chromosomal evolution analysis indicated a possible whole-genome triplication event in the evolutionary history of U. parvifolia. To study the differing susceptibility of U. parvifolia and U. americana to Dutch elm disease, we inoculated both elms with Ceratocystis ulmi and performed comparative transcriptomes analyses at 48, 96, and 144 hours post-inoculation. The results showed that several plant defense and immune response pathways were more highly expressed in U. parvifolia at 48 and 96 hours post-inoculation, implying a potential genetic basis for its higher resistance to Dutch elm disease. Our study represents an advancement in the genomic understanding of U. parvifolia, and especially sheds light on the genetic underpinnings of disease resistance in elms, and provides a foundation for future research into elm breeding for disease resistance and conservation efforts.

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Unraveling the transcriptional features and gene expression networks of pathogenic and saprotrophic Ophiostoma species during the infection of Ulmus americana

Cited by 2 publications

References 99 publications

Distinct biogeographic patterns for bacteria and fungi in association with Bursaphelenchus xylophilus nematodes and infested pinewood

Distinct biogeographic patterns for bacteria and fungi in association with Bursaphelenchus xylophilus nematodes and infested pinewood

Chromosome-Level Genome Assembly Unveils the Molecular Mechanisms Underlying Disease Resistance in Ulmus parvifolia

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