Unravelling calcium-alleviated aluminium toxicity in Arabidopsis thaliana: Insights into regulatory mechanisms using proteomics

Chen, Juan; Duan, Ruixue; Hu, Wenjun; Zhang, Ni-Na; Lin, Xue‐Yuan; Zhang, Jianhua; Zheng, Hai-Lei

doi:10.1016/j.jprot.2019.02.013

Cited by 22 publications

(8 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The environmental stresses severely affect the material metabolism of plants ( Liu et al, 2011a ; Chen et al, 2014 ). UDP-glucose pyrophosphorylase is the precursor of carbohydrate formation ( Chen et al, 2019 ). Our results showed that Ca application reduced AR-induced increases in protein abundance of UDP-glucose pyrophosphorylase (spot 2) in T. wallichiana var.…”

Section: Discussionmentioning

confidence: 99%

“…These results showed that Ca application helps in alleviating the adverse effects of AR stress by accelerating the carbohydrate metabolism. On the other hand, Chen et al (2019) found that UDP-glucose pyrophosphorylase plays an important role in cell wall metabolism and the synthesis of cellulase and hemicellulose. Carbohydrates act as a cytoskeleton and compose the cell wall as the first physical barrier defending against all types of AR stress ( Liu et al, 2011b ).…”

Section: Discussionmentioning

confidence: 99%

“…This change meant that less damaged protein is produced in high Ca condition, by which we furthermore find that the recovered photosynthesis decline caused by AR stress. Moreover, H + -ATPase plays an important role in the maintenance of ion homeostasis and nutrient uptake in plants ( Liu et al, 2011b ; Chen et al, 2019 ). Our study also showed that plasma membrane H + -ATPase (spot 11) was upregulated by high Ca level with AR treatment.…”

Section: Discussionmentioning

confidence: 99%

“…As stress response proteins and heat shock proteins can be induced by many abiotic stresses ( Wang et al, 2004 , 2013 ), our study showed that exogenous Ca addition enhanced the abundance of heat shock protein (spot 19) and heat shock protein 70 (spot 20). Additionally, heat shock proteins are also considered as an important part in stabilizing protein folding and play important roles in the cellular stress response ( Chen et al, 2019 ). The increased abundance of the heat shock-related proteins in high Ca-AR treatment set indicated the multidimensional role of external Ca supply in the alleviation of AR stress in T. wallichiana var.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Physiological, Proteomic Analysis, and Calcium-Related Gene Expression Reveal Taxus wallichiana var. mairei Adaptability to Acid Rain Stress Under Various Calcium Levels

Hu¹,

Liu

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

As one of the serious environmental problems worldwide, acid rain (AR) has always caused continuous damage to the forestry ecosystem. Studies have shown that AR can leach calcium ions from plants and soil. Calcium (Ca) is also a crucial regulator of the plant stress response, whereas there are few reports on how Ca regulates the response of AR-resistant woody plants to AR stress. In this study, by setting different exogenous Ca levels, we study the physiological and molecular mechanism of Ca in regulating the Taxus wallichiana var. mairei response to AR stress. Our results showed that low Ca level leads to photosynthesis, and antioxidant defense system decreases in T. wallichiana var. mairei leaves; however, these negative effects could be reversed at high Ca level. In addition, proteomic analyses identified 44 differentially expressed proteins in different Ca level treatments of T. wallichiana var. mairei under AR stress. These proteins were classified into seven groups, which include metabolic process, photosynthesis and energy pathway, cell rescue and defense, transcription and translation, protein modification and degradation, signal transduction, etc. Furthermore, the study found that low Ca level leads to an obvious increase of Ca-related gene expression under AR stress in T. wallichiana var. mairei using qRT-PCR analyses and however can be reversed at high Ca level. These findings would enrich and extend the Ca signaling pathways of AR stress in AR-resistant woody plants and are expected to have important theoretical and practical significance in revealing the mechanism of woody plants tolerating AR stress and protecting forestry ecosystem in soil environment under different Ca levels.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Physiological, Proteomic Analysis, and Calcium-Related Gene Expression Reveal Taxus wallichiana var. mairei Adaptability to Acid Rain Stress Under Various Calcium Levels

Hu¹,

Liu

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In some instances, aluminum can limit plant growth in acidic soils. Proteomic studies show that adding calcium can reduce the growth inhibition of aluminum on Arabidopsis thaliana (Chen, Duan, et al, 2019).…”

Section: Application In Soil Environmental Pollution Controlmentioning

confidence: 99%

Molecular biological methods in environmental engineering

Zhang

et al. 2020

Water Environment Research

View full text Add to dashboard Cite

Microbes are sensitive to environmental changes and can respond in a short time. Genomics, proteomics, transcriptomics, metabolomics, and multigroup association are used to characterize the composition, function, and metabolism of microorganisms, and to evaluate the environment according to the changes in microorganisms, which has important reference and guiding significance of environmental monitoring, management, and repair. In this paper, the application of molecular biological methods to study environmental microorganisms in the fields of wastewater treatment, pollution control, soil improvement, and environmental monitoring in 2019 is reviewed.

show abstract