Unravelling geospatial distribution and genetic diversity of greenhouse whitefly, Trialeurodes vaporariorum (Westwood) from Himalayan Region

Paschapur, Amit Umesh; Singh, Ashish Kumar; Buski, Ramesh; Guru, P.  N.; Jeevan, B.; Mishra, Krishna Kant; Kant, Lakshmi

doi:10.1038/s41598-023-37781-y

Cited by 3 publications

(3 citation statements)

References 40 publications

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“…The species T. vaporariorum, known to inhabit greenhouses (Moraes et al, 2018;Perring et al, 2018), has now been discovered in open field conditions within cabbage, common bean, and passion fruit crops. This finding corroborates observations made by Lourenção et al (2008), who reported the presence of T. vaporariorum in vegetables in the state of São Paulo, and Paschapur et al (2023), who noted its natural occurrence in India. There have been reports of NW in Argentina, the United States, Martinique, Mexico, and Venezuela (Barbosa et al, 2014b).…”

Section: Resultssupporting

confidence: 92%

Whitefly distribution and interaction with endosymbionts in the state of Paraná

Tomaz,

Cobianchi,

Lima

et al. 2023

Sem. Ci. Agr.

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Whiteflies comprise a complex of cryptic species that are genetically distinct but morphologically indistinguishable. Among them, Bemisia tabaci and Trialeurodes vaporariorum (Tvap) are the most prominent species causing agricultural damage. In Brazil, three B. tabaci species have been identified: New World (NW), Middle East-Asia Minor (MEAM1), and Mediterranean (MED). Whiteflies establish symbiotic associations with endosymbiotic bacteria, conferring adaptive advantages to the insects. The objective of this study was to identify whitefly species in municipalities of the state of Paraná and investigate their interactions with endosymbiotic bacteria. Insects were collected in 14 municipalities across different edaphoclimatic regions of Paraná, either in agricultural crops or on weeds. Molecular analyses, based on previously published PCR-based methods, were used to identify the insects and their respective endosymbionts. The results revealed that B. tabaci MEAM1, MED, NW, and Tvap accounted for 39.8%, 27.0%, 6.3%, and 23.7% of the collected specimens, respectively. These insects exhibited interactions with endosymbiont genera such as Portiera, Arsenophonus, Rickettsia, Hamiltonella, and Wolbachia, either in isolation or through co-infection. In some instances, interactions with up to five endosymbionts were observed in specific locations. The occurrence of Tvap in open fields and the prevalence of MED, particularly in the southeast of the state, where temperatures are milder, along with their interactions with endosymbionts providing adaptive advantages to the insects, raise significant phytosanitary concerns for the agricultural security of Paraná.

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Section: Resultssupporting

confidence: 92%

Whitefly distribution and interaction with endosymbionts in the state of Paraná

Tomaz,

Cobianchi,

Lima

et al. 2023

Sem. Ci. Agr.

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“…T. acaciae was recorded in India on subabul, with subsequent infestations observed on orchid trees, tamarind, rain trees and an unidentified pulse crop (Sundararaj and Vimala, 2018). The impact of T. vaporariorum extends to vegetables and ornamental species, requiring comprehensive management strategies, as outlined by Paschapur et al (2023).…”

Section: O N L I N Ementioning

confidence: 99%

“…The shift in damaging patterns, transitioning from greenhouses to open fields, raises significant concerns (Paschapur et al, 2023). A. floccissimus primarily infests citrus trees and may also feed on guava, mango, litchi and ornamental plants (Sundararaj et al, 2020).…”

Section: O N L I N Ementioning

confidence: 99%

Unravelling genetic diversity of whitefly species on different host plants from New Delhi

Rudra Gouda,

Rajna,

Gambhir

et al. 2024

JEB

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Aim: This study investigates the genetic diversity and distribution of different species of whiteflies on 20 host plants vegetables (cucumber, summer squash, pumpkin, tomato, brinjal, okra), field crops (sunflower, lettuce, field bean, lobia), commercial crops (cotton, tobacco), ornamentals (chrysanthemum, bellflower, marigold), fruit crops (lemon), trees (mulberry, wild tamarind, ficus), and others (wild brinjal) in New Delhi. Methodology: Genetic diversity was assessed through mitochondrial cytochrome oxidase-I gene-based molecular markers. Results: Phylogenetic analysis revealed that whitefly Bemisia tabaci was the most prevalent pest species across the crops reported in this study. The other whitefly species, viz., Dialeurodes sp., Singhiella simplex, Aleuroclava sp., Tetraleurodes acaciae, Trialeurodes vapororium, and Aleurodicus floccissimus were reported as pests of different horticultural crops. Interpretation: Genetic diversity of B. tabaci revealed that the genetic groups Asia II-1 and Asia II-7 were most prevalent, while Asia I was the minor one. Understanding the species composition and genetic diversity of whiteflies will help in devising appropriate control strategies. Key words: Hosts, Molecular markers, mtCOI, New Delhi, Phylogenetic analysis, Whitefly diversity

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sangerFlow, a Sanger sequencing-based bioinformatics pipeline for pests and pathogens identification

Prodhan,

Power,

Kehoe

2024

Preprint

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Sequencing of a Polymerase Chain Reaction product (amplicon) is called amplicon sequencing. Amplicon sequencing allows for reliable identification of an organism by amplifying, sequencing, and analysing a single conserved marker gene or DNA barcode. As this approach generally involves a single gene, it is a light-weight protocol compared to multi-locus or whole genome sequencing for diagnostic purposes; yet considerably reliable. Therefore, Sanger-based high-quality amplicon sequencing is widely deployed for species identification and high-throughput biosecurity surveillance. However, keeping up with the data analysis in a large-scale surveillance or diagnostic settings could be a limiting factor because it involves manual quality control of the raw sequencing data, alignment of the forward and reverse reads, and finally web-based Blastn search of all the amplicons. Here, we present a bioinformatics pipeline that automates the entire analysis. As a result, the pipeline is scalable with high-volume of samples and reproducible. Furthermore, the pipeline leverages the modern open-source Nextflow and Singularity concept, thus it does not require software installation except Nextflow and Singularity, software subscription, or programming expertise from the end users making it widely adaptable.

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Unravelling geospatial distribution and genetic diversity of greenhouse whitefly, Trialeurodes vaporariorum (Westwood) from Himalayan Region

Cited by 3 publications

References 40 publications

Whitefly distribution and interaction with endosymbionts in the state of Paraná

Whitefly distribution and interaction with endosymbionts in the state of Paraná

Unravelling genetic diversity of whitefly species on different host plants from New Delhi

sangerFlow, a Sanger sequencing-based bioinformatics pipeline for pests and pathogens identification

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