Unravelling the blood supply to the zebrafish pharyngeal jaws and teeth

Crucke, Jeroen; Huysseune, Ann

doi:10.1111/joa.12096

Cited by 14 publications

(17 citation statements)

References 71 publications

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“…This corresponds with the observation that the branch of the hypobranchial artery that supplies the dentition reaches the dentigerous area at only 6 d postfertilization (Crucke and Huysseune 2013). This is already an indication that first-generation teeth probably develop independently from the vasculature.…”

Section: Discussionsupporting

confidence: 67%

Blocking VEGF Signaling Delays Development of Replacement Teeth in Zebrafish

Crucke

Huysseune

2014

J Dent Res

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The dentition in zebrafish is extremely and richly vascularized, but the function of the vasculature, in view of the continuous replacement of the teeth, remains elusive. Through application of SU5416, a vascular endothelial growth factor receptor inhibitor, we studied the role of the blood vessels in the dentition of the zebrafish. We were unable to show an effect on the development of first-generation teeth as well as first tooth replacement. However, in juvenile fish, a delay was observed in the developmental state of the replacement tooth compared with what was expected based on the maturation state of the functional tooth. Furthermore, we observed a difference between treated and nontreated fish in the distance between blood vessels and developing replacement teeth. In conclusion, our results provide support for a nutritive, rather than an inductive, function of the vasculature in the process of tooth development and replacement.

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Section: Discussionsupporting

confidence: 67%

Blocking VEGF Signaling Delays Development of Replacement Teeth in Zebrafish

Crucke

Huysseune

2014

J Dent Res

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“…). Given a possible role of motor fibres in regulating blood flow, it is important to note that the dentigerous area in zebrafish is richly supplied with blood (Crucke & Huysseune, ). The hypothesis has recently been raised that blood vessels act instructively to initiate tooth development in zebrafish (Crucke & Huysseune, ).…”

Section: Discussionmentioning

confidence: 99%

“…At this stage, teeth become functional allowing nerves to serve their function for the tooth. Likewise, blood vessels could only be seen penetrating at the base of replacement teeth at very late stages of differentiation (Crucke & Huysseune, ), further suggesting a developmental and/or functional link between both. Moreover, the initiation of a replacement tooth (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…In mammals, axons present in the pulp take part in the regulation of pulpal blood flow (Kim, 1990;Kerezoudis et al 1992;Olgart, 1996;Pagella et al 2014). Given a possible role of motor fibres in regulating blood flow, it is important to note that the dentigerous area in zebrafish is richly supplied with blood (Crucke & Huysseune, 2013). The hypothesis has recently been raised that blood vessels act instructively to initiate tooth development in zebrafish (Crucke & Huysseune, 2013).…”

Section: Branches Of the Vagal Nerve Innervate The Pharyngeal Jawsmentioning

confidence: 99%

“…Given a possible role of motor fibres in regulating blood flow, it is important to note that the dentigerous area in zebrafish is richly supplied with blood (Crucke & Huysseune, 2013). The hypothesis has recently been raised that blood vessels act instructively to initiate tooth development in zebrafish (Crucke & Huysseune, 2013). However, in a follow-up study, it was demonstrated that blood vessels are not required for tooth initiation, but rather allow the teeth to further grow and develop (Crucke & Huysseune, 2015).…”

Section: Branches Of the Vagal Nerve Innervate The Pharyngeal Jawsmentioning

confidence: 99%

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The innervation of the zebrafish pharyngeal jaws and teeth

2015

Self Cite

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Zebrafish (Danio rerio) teeth are increasingly used as a model to study odontogenesis in non-mammalians. Using serial semi-thin section histology and immunohistochemistry, the nerves innervating the pharyngeal jaws and teeth have been identified. The last pair of branchial arches, which are non-gill bearing but which carry the teeth, are innervated by an internal branch of a post-trematic ramus of the vagal nerve. Another, external, branch is probably responsible for the motor innervation of the branchiomeric musculature. Nerve fibres appear in the pulp cavity of the teeth only late during cytodifferentiation, and are therefore likely not involved in early steps of tooth formation. The precise role of the nervous system during continuous tooth replacement remains to be determined. Nonetheless, this study provides the necessary morphological background information to address this question.

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Anatomy and Ontogeny of a Novel Hemodynamic Organ in Zebrafish

Binelli

Luna

LeClair

2014

The Anatomical Record

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The zebrafish maxillary barbel can protract and retract in response to stimuli, and appears connected to a prominent blood sinus on the lateral aspect of the maxillary bone. However, the mechanism of barbel movement is not described. Using whole-mount phalloidin staining of the sinus region, we observed long filamentous actin cables, suggesting highly organized vascular smooth muscle cells, surrounding an endothelial chamber. Although the chamber is variably filled by erythrocytes in vivo, cardiac injection of fluorescent dextrans shows that it consistently contains plasma. Full-thickness confocal imaging of dextran-injected adults containing EGFP+ endothelial cells revealed a vascular complex with three compartments, here named the distal bulb, central chamber, and accessory chamber. The early ontogeny of all three compartments was confirmed in a whole-mount series of Tg(fli1a:EGFP) juveniles. In wild type adults, the fine structure of each chamber was studied using paraffin- and plastic-section histochemistry and transmission electron microscopy. The distal bulb and central chamber have smooth muscle coats with luminally-elongated septa, forming semi-detached blood-filled lacunae. The central chamber walls and septa are extensively innervated by small, unmyelinated axons, as confirmed by immunohistochemical detection of acetylated tubulin, a component of axonal cytoplasm. The accessory chamber appears neither innervated nor muscularized, but is an endothelial cul-de-sac with a thickened elastic adventitia, suggesting an extensible fluid reservoir. We propose that we have identified a new organ in zebrafish, the maxillary barbel blood sinus, whose neurovascular specializations may contribute to zebrafish sensory biology and appendage control.

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Unravelling the blood supply to the zebrafish pharyngeal jaws and teeth

Cited by 14 publications

References 71 publications

Blocking VEGF Signaling Delays Development of Replacement Teeth in Zebrafish

Blocking VEGF Signaling Delays Development of Replacement Teeth in Zebrafish

The innervation of the zebrafish pharyngeal jaws and teeth

Anatomy and Ontogeny of a Novel Hemodynamic Organ in Zebrafish

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