Unravelling the neuropeptidome of the ornate spiny lobster Panulirus ornatus: A focus on peptide hormones and their processing enzymes expressed in the reproductive tissues

Glendinning, Susan; Fitzgibbon, Quinn P.; Smith, Gregory G.; Ventura, Tomer

doi:10.1016/j.ygcen.2022.114183

General and Comparative Endocrinology

2023

DOI: 10.1016/j.ygcen.2022.114183

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Unravelling the neuropeptidome of the ornate spiny lobster Panulirus ornatus: A focus on peptide hormones and their processing enzymes expressed in the reproductive tissues

Susan Glendinning¹,

Quinn P. Fitzgibbon²,

Gregory G. Smith³

et al.

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2024

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Cited by 4 publications

References 100 publications

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microRNA‐8514‐5p regulates adipokinetic hormone/corazonin‐related peptide receptor to affect development and reproduction of Plutella xylostella

Pang,

Wang,

Asad

et al. 2024

Pest Management Science

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BACKGROUNDDicer1 plays a crucial role in regulating the development and reproduction of insects. Knockout of Dicer1 causes pupal deformity, low eclosion and low fecundity in Plutella xylostella, but the mechanism behind this phenomenon is not clear. This study aims to identify differentially‐expressed genes and miRNAs in the Dicer1‐knockout strain (ΔPxDcr‐1) and assess their impact on the reproduction and development of P. xylostella.RESULTSThe knockout of Dicer1 affected the expression of genes including the adipokinetic hormone/corazonin‐related peptide receptor (PxACPR). The expression of PxACPR was upregulated, and the expression of miR‐8514‐5p was downregulated in ΔPxDcr‐1 of P. xylostella. The dual luciferase reporter assay and pull‐down assay showed that miR‐8514‐5p bound to PxACPR in vitro and in vivo. The expression profiles demonstrated a negative correlation between PxACPR mRNA and miR‐8514‐5p in different developmental stages of the wild‐type strain. Both the miR‐8514‐5p agomir and double‐stranded RNA of ACPR (dsPxACPR) injected into the pre‐pupae inhibited the mRNA level of PxACPR, causing high mortality and deformity of pupae, and low fecundity and hatching rate, which were consistent with the phenotype of ΔPxDcr‐1. The injection of miR‐8514‐5p antagomir caused a similar phenotype to the injection of miR‐8514‐5p agomir. Additionally, the injection of miR‐8514‐5p antagomir significantly rescued the phenotype caused by dsPxACPR.CONCLUSIONThese results indicate that miR‐8514‐5p affects the development and reproduction of P. xylostella by regulating PxACPR, and the homeostasis of PxACPR expression is essential for the development and reproduction of P. xylostella. © 2024 Society of Chemical Industry.

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microRNA‐8514‐5p regulates adipokinetic hormone/corazonin‐related peptide receptor to affect development and reproduction of Plutella xylostella

Pang,

Wang,

Asad

et al. 2024

Pest Management Science

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The gonadotropin-releasing hormone (GnRH) superfamily across Pancrustacea/Tetraconata: A role in metabolism?

Marco,

Glendinning,

Ventura

et al. 2024

Molecular and Cellular Endocrinology

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Evaluation of the Effect of Adipokinetic Hormone/Corazonin-Related Peptide (ACP) on Ovarian Development in the Mud Crab, Scylla paramamosain

Tan,

Tang,

Liu

et al. 2024

Animals

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In this study, we identified Sp-ACP and its putative receptor from the mud crab S. paramamosain and explored its potential role in ovarian development. RT-PCR results suggested Sp-ACP was extensively expressed in nervous tissues, the ovary, the middle gut, and the Y-organ, while Sp-ACPR was highly expressed in the ovary. The expression level of Sp-ACP in the ovary, eyestalk, and cerebral ganglia gradually increased during ovarian development, whereas its receptor exhibited an opposite expression pattern in the ovary. Immunofluorescence revealed that ACP was specifically localized in the follicle cells of the ovary. In vitro experiments showed that the expression of vitellogenin receptor (Sp-VgR) in the ovary was significantly increased by 4 and 6 h incubation of Sp-ACP (10 nM). In addition, 12 h injection of Sp-ACP significantly induced the levels of Sp-Vg in the hepatopancreas and Sp-VgR in the ovary, and hemolymph 17β-estradiol titer. Finally, it demonstrated that prolonged injection of Sp-ACP significantly increased the level of Vg and VgR expression, hemolymph 17β-estradiol titer, GSI, and the oocyte diameter. In conclusion, our results suggested that ACP is involved in the regulation of ovarian development of S. paramamosain, likely by inducing hepatopancreas Sp-Vg expression through estradiol and promoting the uptake of Vg by oocytes.

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Unravelling the neuropeptidome of the ornate spiny lobster Panulirus ornatus: A focus on peptide hormones and their processing enzymes expressed in the reproductive tissues

Cited by 4 publications

References 100 publications

microRNA‐8514‐5p regulates adipokinetic hormone/corazonin‐related peptide receptor to affect development and reproduction of Plutella xylostella

microRNA‐8514‐5p regulates adipokinetic hormone/corazonin‐related peptide receptor to affect development and reproduction of Plutella xylostella

The gonadotropin-releasing hormone (GnRH) superfamily across Pancrustacea/Tetraconata: A role in metabolism?

Evaluation of the Effect of Adipokinetic Hormone/Corazonin-Related Peptide (ACP) on Ovarian Development in the Mud Crab, Scylla paramamosain

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