Unravelling the physiological basis of superficial scald in pears based on cultivar differences

Larrigaudière, Christian; Candan, Ana Paula; Giné-Bordonaba, Jordi; Civello, Marcos; Calvo, G.

doi:10.1016/j.scienta.2016.10.043

Cited by 31 publications

(30 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8a). This result supports the active role of the ethylene inhibitor in promoting the scavenging of ROS via an upregulation of key genes of the glutathione/ascorbate pathway, but also emphasizes, as suggested by others 20 , the protective role that ascorbate may have on the development of this physiological disorder.…”

Section: The Role Of Antioxidants In the Prevention Of Superficial Scaldsupporting

confidence: 86%

“…The application of the chemical antioxidants diphenylamine and ethoxyquin in apples and pears, respectively, was a common practice employed to avoid the appearance of scald, preventing the oxidation of α-farnesene to MHO 20,[44][45][46][47][48] . During the development of scald, the skin of the untreated (CT) samples was characterized by the largest polyphenol accumulation (Fig.…”

Section: The Role Of Antioxidants In the Prevention Of Superficial Scaldmentioning

confidence: 99%

“…Consistent with these observations, a non-ethylene-dependent accumulation of α-farnesene has also been reported in 1-MCP-treated "Blanquilla" pears 3 , which together with other factors (i.e., influence of the fruit maturity at harvest on scald development) 4,18 revealed the complex and likely differential regulation of this physiological disorder when comparing apples and pears. Specific studies conducted on pears have also suggested that changes in the expression of glutathione S-transferases (GSTs) and mainly a downregulation of dehydroascorbate reductase (DHAR) genes were linked to scald development 19 , hence highlighting the action that ascorbate may have on the progress of this physiological disorder 20 . In turn, 1-MCP treatments inhibiting the appearance of scald not only affected the expression of genes related to ethylene metabolism, but also the expression of several GSTs and glutathione peroxidase genes in "Wujiuxiang" pears 21 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Investigation of the transcriptomic and metabolic changes associated with superficial scald physiology impaired by lovastatin and 1-methylcyclopropene in pear fruit (cv. “Blanquilla”)

et al. 2020

Self Cite

View full text Add to dashboard Cite

To elucidate the physiology underlying the development of superficial scald in pears, susceptible "Blanquilla" fruit was treated with different compounds that either promoted (ethylene) or repressed (1-methylcyclopropene and lovastatin) the incidence of this disorder after 4 months of cold storage. Our data show that scald was negligible for the fruit treated with 1-methylcyclopropene or lovastatin, but highly manifested in untreated (78% incidence) or ethylenetreated fruit (97% incidence). The comparison between the fruit metabolomic profile and transcriptome evidenced a distinct reprogramming associated with each treatment. In all treated samples, cold storage led to an activation of a cold-acclimation-resistance mechanism, including the biosynthesis of very-long-chain fatty acids, which was especially evident in 1-methylcyclopropane-treated fruit. Among the treatments applied, only 1-methylcyclopropene inhibited ethylene production, hence supporting the involvement of this hormone in the development of scald. However, a common repression effect on the PPO gene combined with higher sorbitol content was found for both lovastatin and 1-methylcyclopropene-treated samples, suggesting also a non-ethylene-mediated process preventing the development of this disorder. The results presented in this work represent a step forward to better understand the physiological mechanisms governing the etiology of superficial scald in pears.

show abstract

Section: The Role Of Antioxidants In the Prevention Of Superficial Scaldsupporting

confidence: 86%

Section: The Role Of Antioxidants In the Prevention Of Superficial Scaldmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Investigation of the transcriptomic and metabolic changes associated with superficial scald physiology impaired by lovastatin and 1-methylcyclopropene in pear fruit (cv. “Blanquilla”)

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Complex networks and multiple key factors are involved in fruit superficial scald development, such as ethylene metabolism, α-farnesene content, hydroperoxides or conjugated trienols (CTols), reactive oxygen species (ROS), and programmed cell death (PCD); however, other physiological or biochemical processes that determine scald susceptibility are not equivalent in apples and pears [2,[4][5][6]. No positive correlation has been found between ethylene production and scald incidence in pears, as demonstrated in the 'Beurréd'Anjou' pear that contains a low amount of ethylene but high scald susceptibility [7]. Furthermore, several studies have demonstrated that α-farnesene biosynthesis could be not ethylene-dependent but influenced also by pre-and post-harvest factors in different pear cultivars [4,[7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Influence of Cold Storage on Pear Physico-Chemical Traits and Antioxidant Systems in Relation to Superficial Scald Development

Caracciolo¹,

Magri²,

Petriccione³

et al. 2020

Foods

View full text Add to dashboard Cite

Superficial scald is the most common physiological disorder in apples and pears and causes huge economic losses worldwide. The aim of this study is to determine the different scald susceptibilities of seven pear cultivars/selections during five months of cold storage (CS). Four advanced pear selections and three commercial cultivars were harvested from an orchard located in Emilia-Romagna region, and cold stored at −1 °C and 85% relative humidity (RH).After 90, 120, and 150 days, fruits of each cultivar and selection were removed for ripening for 4 d, after which scald damage, physico-chemical and nutraceutical traits, and enzymatic antioxidant systems were evaluated on peel and pulp. ‘Abbé Fétel’, ‘Falstaff’, and ‘CREA 171’ did not showed superficial scald symptoms after 90 days, while ‘Doyenne du Comice’ and ‘CREA 264’ showed the highest susceptibility. After 90 days, CS ‘Falstaff’ and ‘CREA 179’ showed the highest total polyphenol content (TPH) in peel, followed by ‘Doyenne du Comice’ and ‘AbbéFétel’; lowest TPH was detected in ‘CREA 264’. After 120 and 150 days of CS, ‘Abbé Fétel’ and ‘CREA 171’ showed the highest peel TPH. ‘CREA 264’ and ‘CREA 125’ reached the lowest values of TPH during the three CS time periods. Superoxide dismutase and catalase activities were higher in the peel of scald-resistant than that in scald-susceptible pear cultivars/advanced selections. Superficial scald induced an increase in polyphenoloxidase, and guaiacol peroxidase activities involved in fruit-browning during CS. Furthermore, we observed an increase in lipoxygenase activity and consequent membrane damage in both the peel and flesh of the fruit. This study indicates that pear cultivars/advanced selections have different superficial scald susceptibilities that enable them to induce the activity of several antioxidant enzymes, following CS.

show abstract

“…Our understanding of the molecular mechanisms of postharvest tree fruit disorders is continuously evolving (Johnson and Zhu 2015;Leisso et al, 2015;Lum et al, 2016;Sevillano et al, 2009). Disorder incidence and severity is often cultivar dependent, with some cultivars being predisposed to specific physiological defects over the course of long-term storage (Larrigaudi ere et al, 2016). Examining changes in gene expression during the postharvest period will 1) shed light on the molecular mechanisms underlying the physiology of tree fruit disorders; 2) be useful in classifying disorder susceptibility; 3) act as a guide for new storage strategies; and 4) improve risk assessment and management before, during, and after storage (Duan et al, 2017;Leisso et al, 2016;Nham et al, 2015).…”

mentioning

confidence: 99%

Leveraging Transcriptome Data for Enhanced Gene Expression Analysis in Apple

Hargarten¹,

Waliullah²,

Kalcsits³

et al. 2018

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

Complex changes in gene expression occur during postharvest storage of apple (Malus ×domestica) and often precede or accompany changes in ripening and disorder development. Targeted gene expression analysis fundamentally relies on previous knowledge of the targeted gene. Minimally, a substantial fragment of the gene sequence must be known with high accuracy so that primers and probes, which bind to their targets in a complimentary fashion, are highly specific. Here, we describe a workflow that leverages publicly available transcriptome data to discover apple cultivar–specific gene sequences to guide primer design for quantitative real-time polymerase chain reaction (qPCR). We find that problematic polymorphisms occur frequently in ‘Granny Smith’ and ‘Honeycrisp’ apple when candidate primer binding sites were selected using the ‘Golden Delicious’ genome. We attempted to validate qPCR-based gene expression measurements with RNA sequencing (RNA-Seq) analysis of the same RNA samples. However, we found that agreement between the two technologies was highly variable and positively correlated with the similarity between cultivar-specific genes and RNA-Seq reference genes. Thus, we offer insight that 1) improves the accuracy and efficiency of qPCR primer design in cultivars that lack sufficient sequence resources and 2) better guides the essential step of validation of RNA-Seq data with a subset of genes of interest examined via qPCR.

show abstract

Unravelling the physiological basis of superficial scald in pears based on cultivar differences

Cited by 31 publications

References 44 publications

Investigation of the transcriptomic and metabolic changes associated with superficial scald physiology impaired by lovastatin and 1-methylcyclopropene in pear fruit (cv. “Blanquilla”)

Investigation of the transcriptomic and metabolic changes associated with superficial scald physiology impaired by lovastatin and 1-methylcyclopropene in pear fruit (cv. “Blanquilla”)

Influence of Cold Storage on Pear Physico-Chemical Traits and Antioxidant Systems in Relation to Superficial Scald Development

Leveraging Transcriptome Data for Enhanced Gene Expression Analysis in Apple

Contact Info

Product

Resources

About