Unravelling the potential of seaweeds from the Black Sea coast of Romania as bioactive compounds sources. Part I: Cystoseira barbata (Stackhouse) C. Agardh

Trifan, Anca; Vasincu, Alexandru; Luca, Simon Vlad; Neophytou, Christiana M.; Wolfram, Evelyn; Opitz, Sebastian; Sava, Daciana; Bucur, Laura; Cioroiu, Bogdan I.; Miron, Anca; Aprotosoaie, Ana Clara; Cioancă, Oana; Hăncianu, Monica; Jităreanu, Alexandra; Constantinou, Andreas I.

doi:10.1016/j.fct.2019.110820

Cited by 22 publications

(23 citation statements)

References 57 publications

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“…These results are in line with previous works that highlighted the ability of phlorotannins and/or phlorotannin extracts from other seaweeds to induce necrosis and/or apoptosis on multiple tumor cell lines [ 9 , 30 , 31 ] and even in vivo [ 6 ]. For instance, dieckol isolated from E. cava was shown to trigger early and late apoptosis in SKOV3 ovarian cancer cells [ 9 ], MCF-7 breast cancer cells [ 28 ], and A549 non-small lung cancer cells [ 13 ], while induction of both early and late apoptosis was reported in Panc89 pancreatic cancer cells exposed to a hydrophilic fraction isolated from a F. vesiculosus 70% acetone extract for 24 h [ 10 ].…”

Section: Resultssupporting

confidence: 92%

“…Induction of necrosis is, however, a mechanism that is not usually observed for these compounds, although two studies carried out with Cystoseira seaweeds reported a similar behavior. In more detail, the exposure of MCF-7 breast cancer cells to C. barbata 70% acetone extract over 48 h caused a dose-dependent increase not only in the number of apoptotic cells (both early and late), but also in the necrotic cells [ 31 ], while in HL60 and THP-1 cells, the treatment with C. tamariscifolia methanol extracts significantly increased the percentages of late apoptotic and necrotic cells, but not the percentages of the early apoptotic cells [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…In more detail, the exposure of MCF-7 breast cancer cells to C. barbata 70% acetone extract over 48 h caused a dose-dependent increase not only in the number of apoptotic cells (both early and late), but also in the necrotic cells [31], while in HL60 and THP-1 cells, the treatment with C. tamariscifolia methanol extracts significantly increased the percentages of late apoptotic and necrotic cells, but not the percentages of the early apoptotic cells [32]. These results are in line with previous works that highlighted the ability of phlorotannins and/or phlorotannin extracts from other seaweeds to induce necrosis and/or apoptosis on multiple tumor cell lines [9,30,31] and even in vivo [6]. For instance, dieckol isolated from E. cava was shown to trigger early and late apoptosis in SKOV3 ovarian cancer cells [9], MCF-7 breast cancer cells [28], and A549 non-small lung cancer cells [13], while induction of both early and late apoptosis was reported in Panc89 pancreatic cancer cells exposed to a hydrophilic fraction isolated from a F. vesiculosus 70% acetone extract for 24 h [10].…”

Section: Apoptosis/necrosis Detection On Cells Treated With F Vesiculosus Samplesmentioning

confidence: 97%

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Antitumor Activity of Fucus vesiculosus-Derived Phlorotannins through Activation of Apoptotic Signals in Gastric and Colorectal Tumor Cell Lines

Catarino

Fernandes

Oliveira

et al. 2021

IJMS

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Seaweeds are one of the largest producers of biomass in the marine environment and a source of multiple bioactive metabolites with valuable health benefits. Among these, phlorotannins have been widely recognized for their promising bioactive properties. The potential antitumor capacity of Fucus vesiculosus-derived phlorotannins remains, however, poorly explored, especially in gastrointestinal tract-related tumors. Therefore, this work aimed to evaluate the cytotoxic properties and possible mechanisms by which F. vesiculosus crude extract (CRD), phlorotannin-rich extract (EtOAc), and further phlorotannin-purified fractions (F1–F9) trigger cell death on different tumor cell lines of the gastrointestinal tract, using flow cytometry. The results indicate that F. vesiculosus samples exert specific cytotoxicity against tumor cell lines without affecting the viability of normal cells. Moreover, it was found that, among the nine different phlorotannin fractions tested, F5 was the most active against both Caco-2 colorectal and MKN-28 gastric cancer cells, inducing death via activation of both apoptosis and necrosis. The UHPLC-MS analysis of this fraction revealed, among others, the presence of a compound tentatively identified as eckstolonol and another as fucofurodiphlorethol, which could be mainly responsible for the promising cytotoxic effects observed in this sample. Overall, the results herein reported contribute to a better understanding of the mechanisms behind the antitumor properties of F. vesiculosus phlorotannin-rich extracts.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Apoptosis/necrosis Detection On Cells Treated With F Vesiculosus Samplesmentioning

confidence: 97%

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Antitumor Activity of Fucus vesiculosus-Derived Phlorotannins through Activation of Apoptotic Signals in Gastric and Colorectal Tumor Cell Lines

Catarino

Fernandes

Oliveira

et al. 2021

IJMS

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“…Over the past decade, considerable attention has been centered on researches related to marine macroalgae as they are the largest reservoir of structurally diverse, bioactive compounds and secondary metabolites (3)(4)(5)(6). Though the anti-inflammatory, anticoagulant, antimicrobial, anticancer, antiviral, antioxidant, hypoglycaemic, antiulcer, neuroprotective, and hepatoprotective properties of seaweeds are well documented, the phytochemicals attributed to these properties are yet to be explored (7)(8)(9)(10)(11)(12). Among marine algae, the brown seaweeds collected from different coasts across the globe have been widely studied.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and anti-inflammatory activities of methanol and aqueous extracts of Sargassum wightii

Rout

Rath

Bhattamisra

et al. 2021

J Herbmed Pharmacol

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Introduction: Antioxidants of natural sources for the treatment of many ailments have taken priority since the last decades. Recently, researches have been focused on marine algae as they are the largest reservoir of bioactive compounds. Hence, the objective of this study was to explore the in-vitro antioxidant and anti-inflammatory activities of methanolic and aqueous extracts of Sargassum wightii. Methods: The total phenolics, flavonoids, and ascorbic acid (AA) contents were evaluated informs of gallic acid equivalent (GAE), rutin equivalent (RUE), and AA equivalent, respectively. The aqueous and methanolic extracts were isolated. The antioxidant activities were explored using2,2-diphenyl-1-picrylhydrazil (DPPH), superoxide dismutase (SOD), hydroxyl radical scavenging, and ferric reducing power assays. The in vitro anti-inflammatory activity was assayed using nitric oxide radical scavenging, inhibition of protein denaturation, and antiproteinase activities. Results: We observed significant changes in DPPH scavenging activity with both methanolicSargassum extract (MSE) and aqueous Sargassum extract (ASE) [IC50: 511.15 µg/mL and 927.05µg/mL, respectively]. Methanolic extract showed a greater SOD scavenging activity [IC50: 369.56µg/mL] and hydroxyl radical scavenging potential [IC50: 668.93 µg/mL] than that of ASE [SOD,IC50: 923.94 µg/mL; hydroxyl ion, IC50: 953.57 µg/mL]. In the Ferric reducing antioxidant power assay, MSE and ASE exhibited absorbance of 0.93±0.12 and 0.59±0.08, respectively, at 1200 µg/mL each. Both methanol and ASEs showed NO– scavenging activity having IC50 in order, AA(96.46 µg/mL) <MSE (963.50 µg/mL) <ASE (1974.88 µg/mL). However, the protein denaturation inhibition and antiproteinase activity of both these extracts at 1000 µg/mL were similar. Conclusion: Sargassum wightii has promising antioxidant and anti-inflammatory activities and could be a potential candidate for drug development targeting oxidative stress-mediated inflammatory diseases.

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“…[9,10] Therefore, identification of novel prophylactic and therapeutic strategies is of great significance. As part of our continuous research interest in finding new phytochemicals that could be used as potential leads for cancer chemoprevention and therapy, [2,11,12] some preliminary investigations on V. nigrum, a less explored species of mullein, were performed. [13] Consequently, the current work investigated the in vitro cytotoxic activity of a crude methanolic extract obtained from V. nigrum and its six fractions in human skin squamous cell carcinoma A431 cells.…”

Section: Introductionmentioning

confidence: 99%

Verbascum nigrum: Cytotoxicity Evaluation in A431 Epidermoid Carcinoma Cells and Untargeted LC‐HR‐MS/MS Metabolite Profiling

Iliescu

Peter

Albert

et al. 2020

Chemistry & Biodiversity

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The crude methanolic extract obtained from Verbascum nigrum aerial parts (VNE) and its six fractions (VNF1-VNF6) were initially screened regarding their effects on the viability of immortalized HaCaT keratinocytes and A431 epidermoid carcinoma cells (MTT assay, 24 h). None of the tested samples affected the viability of HaCaT cells in a concentration range of 25-150 μg/mL. VNE and VNF4 exhibited significant cytotoxic effects in A431 cells, with IC 50 values of 81.92 and 12.27 μg/mL, respectively; the selectivity index was higher than 10 for VNF4. The untargeted LC/HR-MS/MS metabolite profiling led to the tentative annotation of a total number of 23 compounds. Of these, VNE comprised mainly iridoid glycosides (harpagoside, laterioside, acylated aucubin derivatives), whereas VNF4 showed a high abundance of triterpene saponin glycosides (ilwensisaponins A and C, songarosaponins A and B), constituents known for their selective cytotoxic potential.

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Unravelling the potential of seaweeds from the Black Sea coast of Romania as bioactive compounds sources. Part I: Cystoseira barbata (Stackhouse) C. Agardh

Cited by 22 publications

References 57 publications

Antitumor Activity of Fucus vesiculosus-Derived Phlorotannins through Activation of Apoptotic Signals in Gastric and Colorectal Tumor Cell Lines

Antitumor Activity of Fucus vesiculosus-Derived Phlorotannins through Activation of Apoptotic Signals in Gastric and Colorectal Tumor Cell Lines

Antioxidant and anti-inflammatory activities of methanol and aqueous extracts of Sargassum wightii

Verbascum nigrum: Cytotoxicity Evaluation in A431 Epidermoid Carcinoma Cells and Untargeted LC‐HR‐MS/MS Metabolite Profiling

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