Unravelling the proteome of adult rhesus monkey ovaries

He, Hui; Teng, Hui; Zhou, Tao; Guo, Yueshuai; Wang, Gaigai; Lin, Min; Sun, Yujie; Si, Wei; Zhou, Zuomin; Guo, Xuejiang; Huo, Ran

doi:10.1039/c3mb70312f

Cited by 12 publications

(15 citation statements)

References 80 publications

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“…All the predicted target genes were listed in Supplement Table S2. Gene ontology (GO) and pathway analyses were conducted in Gene Ontology Project (http://www.geneontology.org/) and KEGG (http://www.genome.jp/kegg/) to prompt the enriched biological processes (BP), cellular components (CC), molecular functions (MF) and pathways (Supplement Table S2) of these predicted target genes. Some of the enriched BP, CC, MF, and pathways were shown in Figure A‐D.…”

Section: Resultsmentioning

confidence: 99%

MicroRNA expression profiles of neural stem cells following valproate inducement

Peng

et al. 2018

J of Cellular Biochemistry

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Neural stem cells (NSCs) possess self-renewal and multilineage differentiation ability, thus are considered to be a potential source for cell replacement therapy of many nervous system diseases, such as neurodegenerative diseases. Valproate (VPA), a member of histone deacetylase inhibitor family, is an epigenetic regulator and can promote NSCs to differentiate into neurons, nevertheless, the underlying mechanisms of the process remain unclear. MicroRNAs (miRNAs) exert a crucial part in the posttranscriptional regulation of gene expression. Epigenetic mechanisms involve in the regulation of miRNAs expression. Therefore we speculated that miRNAs may be important factors during the promotion of neuronal differentiation by VPA. Here, after selecting appropriate concentration and treatment time of VPA, we conducted microRNA arrays at 24 h on the treatment of 1 mM VPA or vehicle. After validation, we obtained 5 significantly upregulated miRNAs (miR-29a-5p, miR-674-5p, miR-155-5p, miR-652-3p, and miR-210-3p) in VPA group compared with control. We predicted the target genes of these miRNAs on the website. Through gene ontology (GO) and pathway analyses, we obtained preliminary comprehension of the function of these genes. The bioinformatics analyses indicated the involvement of them during neurogenesis. In addition, we observed high expression of miR-210-3p, miR-29a-5p, and miR-674-5p in central nervous system, which suggested that they were likely to play crucial roles in neuronal differentiation. We then defined the upregulation of Map2 by transfecting mimic of miR-674-5p, which indicated the promotion of miR-674-5p on NSCs differentiation. The present study explored the miRNAs potentially mediated the function of VPA on promoting NSCs to differentiate into neurons.

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Section: Resultsmentioning

confidence: 99%

MicroRNA expression profiles of neural stem cells following valproate inducement

Peng

et al. 2018

J of Cellular Biochemistry

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“…Hence, it is important to obtain a deeper understanding of the regulatory molecules and mechanisms in early pregnancy (Dey et al., ; He et al., ). Given the observation from previous reports that USP7 knockout mice had a dramatic reduction in proliferation and embryonic development between days E6.5 and E7.5 post coitum (He et al., ; Scherle et al., ), we designed experiments to clarify its function in uterine stroma decidualization, a key event in pregnancy. USP7 was observed to be localized at the decidual zone in the uteri and its spatiotemporal localization becomes more prominent as pregnancy progresses (Figures and ).…”

Section: Discussionmentioning

confidence: 99%

“…USP7 was observed to play an essential role in growth and development as it was associated with embryonic lethality in USP7 knockout mice (He et al., ; Scherle, Ma, Lim, Dey, & Trzaskos, ). Hence, we hypothesize that USP7 may play a role in pregnancy, specifically the processes involving embryo implantation to the uterine wall.…”

Section: Introductionmentioning

confidence: 99%

“…It plays a critical role in vital intracellular processes such as epigenetic regulation, cell cycle regulation, cell growth and survival (Gallo & Dirks, 2016;Glasser & Julian, 1986;Hernandez-Perez et al, 2017;Ma & Yu, 2016;Roby et al, 1993). Of note, its role in stabilizing various tumor suppressors, such as p53, Mdm2, PTEN and Myc (Castracane & Jordan, 1975;He et al, 2014;Hernandez-Perez et al, 2017;Hussain et al, 2009;Ma & Yu, 2016;Roby et al, 1993), has been well-characterized and its upregulation has been associated with many cancers (Hernandez-Perez et al, 2017;Jiang et al, 2016;Roby et al, 1993;Turnbull et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Ubiquitin‐specific protease 7 (USP7) is essential for endometrial stromal cell decidualization in mice

et al. 2019

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Ubiquitin‐specific protease 7 (USP7), a member of the deubiquitinating (DUB) enzyme family, regulates protein stability and has a well‐characterized function in tumorigenesis. Given its critical role in growth and development, it was speculated to be involved in modulating processes in the female reproductive system but its exact role has not been elucidated. Decidualization is one of the key processes in pregnancy and aberrant decidualization is a cause of pregnancy failure. The uterine endometrium layer undergoes significant structural and functional changes during decidualization in preparation for and after embryo implantation. Here, we hypothesized that USP7 could be involved in mediating endometrial stromal cell (ESC) decidualization and set out to determine its function with a primary stromal cell culture. Using in situ hybridization and immunohistochemical techniques, we observed increased USP7 expression during uterine decidualization and found that it was predominantly localized to the decidual zone in the post‐implantation uterus. Since the ovarian hormones, progesterone (P4) and estrogen (E2), function in promoting stroma decidualization, we investigated their relationship with USP7 expression and found that they exert minimal influence. Moreover, increased USP7 expression observed during deciduoma development was found to be independent of blastocyst attachment. Using a specific USP7 inhibitor, HBX19818, we demonstrated an additional novel role for USP7 in endometrial stroma decidualization in mice during early pregnancy. Our findings could potentially be applied towards future research and development in female infertility.

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“…Tissues were fixed in 10% neutral buffered formalin or 4% paraformaldehyde, dehydrated via graded ethanol solutions, and embedded in paraffin. The procedure of immunohistochemistry analysis was described previously [39]. Briefly, the sections were subjected to a high-pressure antigen retrieval technique and the endogenous peroxidase activity was quenched.…”

Section: Immunostainingmentioning

confidence: 99%

Rbbp7 Is Required for Uterine Stromal Decidualization in Mice1

Kong

Liu

et al. 2015

Biology of Reproduction

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Uterine stromal cells undergo extensive proliferation and differentiation during postimplantation development, a process known as decidualization. While a range of signaling molecules have been demonstrated to play essential roles in this event, its potential epigenetic regulatory mechanisms remain largely unknown. Retinoblastoma binding protein 7 (Rbbp7) is a protein reported as a core component of many histone modification and chromatin remodeling complexes. In the present study, our in situ hybridization and immunochemistry analysis first reveals a spatiotemporal expression of Rbbp7 in the uterus during the peri-implantation period. Observations of remarkable induction of Rbbp7 expression in uterine stromal cells in response to progesterone-nuclear receptor PR signaling point to its potential physiological significance during postimplantation uterine development. Employing a stealth RNA knockdown approach, combined with primary murine uterine stromal cell culture and an in vitro-induced decidualization model, we further demonstrate that Rbbp7 silencing compromises stromal cell decidualization via attenuating histone H4 acetylation and cyclin D3 expression. The results collectively suggest that Rbbp7 is a potentially functional player regulating normal histone acetylation modification and cyclin D3 expression in stromal cells during postimplantation decidual development.

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Unravelling the proteome of adult rhesus monkey ovaries

Cited by 12 publications

References 80 publications

MicroRNA expression profiles of neural stem cells following valproate inducement

MicroRNA expression profiles of neural stem cells following valproate inducement

Ubiquitin‐specific protease 7 (USP7) is essential for endometrial stromal cell decidualization in mice

Rbbp7 Is Required for Uterine Stromal Decidualization in Mice1

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