Unravelling the regulatory role of miRNAs in secondary metabolite production in medicinal crops

Chakraborty, Shreya; Kumar, Prasann; Sanyal, Rupa; Mane, Abhijit Bhagwan; Prasanth, Dorairaj Arvind; Patil, Mukundraj V.; Dey, Abhijit

doi:10.1016/j.plgene.2021.100303

Cited by 26 publications

(6 citation statements)

References 76 publications

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“…Phenylpropanoid metabolites biosynthesis is a complex network producing various important secondary metabolites, including lignans, and its regulatory mechanism is important for plant growth, development, and (a)biotic stress protection [ 1 ]. The most recent studies are focused on unraveling the miRNAs regulatory potential in the biosynthetic pathways of major secondary metabolites such as alkaloids, terpenoids, and flavonoids [ 1 , 2 , 3 , 4 ]. Lignans are polyphenolic compounds that are widely distributed in plant species.…”

Section: Introductionmentioning

confidence: 99%

“…It is known that miRNAs also participate in phenylpropanoid biosynthesis by targeting transcription factors [ 1 ]. Therefore, the knowledge of the miRNA-based regulatory mechanisms of their biosynthesis is extremely important [ 2 , 3 ]. Currently, there is an effort to increase the production of secondary metabolites, which are produced by medicinal plants in small quantities and are used as insecticides, dyes, drugs, and toxins in agriculture, medicine, and industry.…”

Section: Introductionmentioning

confidence: 99%

“…MiRNA and transcription factors involved in the phenylpropanoid biosynthesis regulation with focus on lignin/lignans target biosynthesis (in italics), elaborated based on references[1][2][3][4]31,33,35,36,46,57,68,[94][95][96][97][98][99][100][101][102][103].…”

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confidence: 99%

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The Involvement of microRNAs in Plant Lignan Biosynthesis—Current View

Ražná¹,

Harenčár²,

Kučka³

2022

Cells

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Lignans, as secondary metabolites synthesized within a phenylpropanoid pathway, play various roles in plants, including their involvement in growth and plant defense processes. The health and nutritional benefits of lignans are unquestionable, and many studies have been devoted to these attributes. Although the regulatory role of miRNAs in the biosynthesis of secondary metabolites has been widely reported, there is no systematic review available on the miRNA-based regulatory mechanism of lignans biosynthesis. However, the genetic background of lignan biosynthesis in plants is well characterized. We attempted to put together a regulatory mosaic based on current knowledge describing miRNA-mediated regulation of genes, enzymes, or transcription factors involved in this biosynthesis process. At the same time, we would like to underline the fact that further research is necessary to improve our understanding of the miRNAs regulating plant lignan biosynthesis by exploitation of current approaches for functional identification of miRNAs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Involvement of microRNAs in Plant Lignan Biosynthesis—Current View

Ražná¹,

Harenčár²,

Kučka³

2022

Cells

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“…Secondary metabolites from plants are a one-of-a-kind source of industrially significant biochemicals, flavors, and medicines [ 1 , 2 ]. Terpenoids are an essential secondary metabolite in plants and are commonly used as chemicals in dyes, flavors, fragrances, insecticides, and drugs by humans thanks to their significant economic worth [ 3 , 4 ]. To control the expression of genes in plants, transcription factors (TFs) coordinate a series of intricate regulatory networks [ 3 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Terpenoids are an essential secondary metabolite in plants and are commonly used as chemicals in dyes, flavors, fragrances, insecticides, and drugs by humans thanks to their significant economic worth [ 3 , 4 ]. To control the expression of genes in plants, transcription factors (TFs) coordinate a series of intricate regulatory networks [ 3 , 5 ]. Because there are many genes encoding different enzymes in the metabolic pathways of plants, manipulating TFs may be easier and more efficient than controlling the expression of the specific enzyme [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

LcWRKY17, a WRKY Transcription Factor from Litsea cubeba, Effectively Promotes Monoterpene Synthesis

Gao

Chen

Gao

et al. 2023

IJMS

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The WRKY gene family is one of the most significant transcription factor (TF) families in higher plants and participates in many secondary metabolic processes in plants. Litsea cubeba (Lour.) Person is an important woody oil plant that is high in terpenoids. However, no studies have been conducted to investigate the WRKY TFs that regulate the synthesis of terpene in L. cubeba. This paper provides a comprehensive genomic analysis of the LcWRKYs. In the L. cubeba genome, 64 LcWRKY genes were discovered. According to a comparative phylogenetic study with Arabidopsis thaliana, these L. cubeba WRKYs were divided into three groups. Some LcWRKY genes may have arisen from gene duplication, but the majority of LcWRKY evolution has been driven by segmental duplication events. Based on transcriptome data, a consistent expression pattern of LcWRKY17 and terpene synthase LcTPS42 was found at different stages of L. cubeba fruit development. Furthermore, the function of LcWRKY17 was verified by subcellular localization and transient overexpression, and overexpression of LcWRKY17 promotes monoterpene synthesis. Meanwhile, dual-Luciferase and yeast one-hybrid (Y1H) experiments showed that the LcWRKY17 transcription factor binds to W-box motifs of LcTPS42 and enhances its transcription. In conclusion, this research provided a fundamental framework for future functional analysis of the WRKY gene families, as well as breeding improvement and the regulation of secondary metabolism in L. cubeba.

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