Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysis

Faizal, Ahmad Mohd; Elias, Marjanu Hikmah; Jin, Norazilah Mat; Abu, Muhammad Azrai; Syafruddin, Saiful Effendi; Zainuddin, Ani Amelia; Suzuki, Nao; Karim, Abdul Kadir Abdul

doi:10.3389/fendo.2024.1274376

Cited by 4 publications

(1 citation statement)

References 55 publications

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“…CC expansion is crucial for good oocyte quality. Several studies show that gene expression at the level of CCs can provide reliable markers for assessing embryo quality, although the correlation needs to be demonstrated more consistently [166]. Electro-metabolic uncoupling could be one of the causes of female infertility as it compromises the mitochondrial functionality essential for the acquisition of oocyte competence [167,168].…”

Section: Clinical Significance Of Electro-metabolic Coupling Of Cocmentioning

confidence: 99%

Electro-Metabolic Coupling of Cumulus–Oocyte Complex

Del Bianco,

Gentile,

Sallicandro

et al. 2024

IJMS

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Oocyte–cumulus cell interaction is essential for oocyte maturation and competence. The bidirectional crosstalk network mediated by gap junctions is fundamental for the metabolic cooperation between these cells. As cumulus cells exhibit a more glycolytic phenotype, they can provide metabolic substrates that the oocyte can use to produce ATP via oxidative phosphorylation. The impairment of mitochondrial activity plays a crucial role in ovarian aging and, thus, in fertility, determining the success or failure of assisted reproductive techniques. This review aims to deepen the knowledge about the electro-metabolic coupling of the cumulus–oocyte complex and to hypothesize a putative role of potassium channel modulators in order to improve fertility, promote intracellular Ca2+ influx, and increase the mitochondrial biogenesis and resulting ATP levels in cumulus cells.

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Section: Clinical Significance Of Electro-metabolic Coupling Of Cocmentioning

confidence: 99%

Electro-Metabolic Coupling of Cumulus–Oocyte Complex

Del Bianco,

Gentile,

Sallicandro

et al. 2024

IJMS

View full text Add to dashboard Cite

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Molecular Biological Approaches to Human Oocyte Developmental Competence Prognosis

Smolyaninova,

Bashendjieva,

Ponomartsev

et al. 2024

Russ J Bioorg Chem

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AREG expression in cumulus cells as a prognostic marker of oocyte quality

Bashendjieva,

Barashkova,

Tatishcheva

et al. 2024

Transl. med. (Print)

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Background. Assisted reproductive technologies (ART) represent the most promising and successful methods of infertility treatment. A personalized approach may enhance its efficacy. One such approach is the development of reliable methods for assessing the quality and selection of embryos for transfer. The quality of the embryo is largely contingent upon the quality of the gametes involved in fertilization; thus, the development of non-invasive methods to assess oocyte quality represents a crucial step in the advancement of personalized ART. It is proposed that molecular and biological characterization of cumulus cells can be utilized to assess oocyte quality and predict the success of implantation of transferred embryos. The aim of the study was to evaluate the expression levels of potential oocyte quality marker genes (AREG, STAR, PTGS2, HAS2 and SCD5) in cumulus cells from healthy donors and patients with primary and secondary infertility. Materials and Methods. Nine donors and 19 patients were enrolled in the study. RNA was isolated from cumulus cells obtained during oocyte preparation for fertilization, and cDNA was synthesized. The cDNA was used as a matrix for real-time PCR with primers for the above-mentioned genes of interest. Results. Significant difference in AREG gene expression was observed between patients with successful (i.e. ended with birth) outcome and with IVF failure. No difference was found for the STAR, HAS2, PTGS2 and SCD5 genes. Conclusion. The method of assessing the expression level of marker genes in cumulus cells by real-time PCR shows considerable promise for the assessment of oocyte quality. The AREG gene is a potential candidate for use as a marker of oocyte quality.

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Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysis

Cited by 4 publications

References 55 publications

Electro-Metabolic Coupling of Cumulus–Oocyte Complex

Electro-Metabolic Coupling of Cumulus–Oocyte Complex

Molecular Biological Approaches to Human Oocyte Developmental Competence Prognosis

AREG expression in cumulus cells as a prognostic marker of oocyte quality

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