Unreduced 2n Pollen Production in ‘Nishiuchi Konatsu’ Hyuganatsu as Inferred by Pollen Characteristics and Progeny Ploidy Level

Honsho, Chitose; Yamamura, Eri; Tsuruta, Kyoko; Yoshimaru, Yukako; Yasuda, Kiichi; Uchida, Asuka; Kunitake, Hisato; Tetsumura, Takuya

doi:10.2503/jjshs1.81.19

Cited by 13 publications

(12 citation statements)

References 37 publications

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“…The hybridization offspring of two lily cultivars ( L. auratum × L. henryi ) produced a number of 2n gametes and were crossed with another lily hybrid (the Oriental hybrid) to produce triploid offspring [40]. The self-pollinated offspring of the diploid Citrus tamurana ‘Nishiuchi Konatsu’ are tetraploids, whereas other combinations of the offspring are diploids [41]. It has been shown that FDR-types form the majority, while IMR (indeterminate meiotic restitution)-types are the minority based on the number of 2n gametes produced by genomic offspring through in situ hybridization (GISH) in lily [37].…”

Section: Discussionmentioning

confidence: 99%

The strong competitive role of 2n pollen in several polyploidy hybridizations in Rosa hybrida

et al. 2019

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Background 2n pollen play a strong competitive role in hybridization and breeding of multiploids in Rosa hybrida . The ploidy inheritable characteristic of ‘Orange Fire’ × ‘Old Blush’ were analyzed. Result The results of the cytological observations indicated that 2n pollen developed from the defeated cytoplasmic division or nuclear division in the meiosis metaphase II of PMC (pollen mother cell) in ‘Old Blush’. The natural generation rate of the 2n pollen in ‘Old Blush’ (2x) was about 1.39 in percentage of all male gametes, whereas the tetraploids in the F1 offspring possessed a high rate, i.e., 44.00%. The temporal and spatial characteristics of ‘Old Blush’ pollen germination on the stigma and growth in pistil of ‘Orange Fire’ and ‘DEE’ were observed, and the results suggested that the germination rate of 2n pollen on the stigma was not superior to that of 1n pollen, but that the proportion of 2n pollen increased to 30.90 and 37.20%, respectively, while it traversed the stigma and entered into style. The callose plug in the 2n pollen tube was significantly thinner than that of 1n pollen tube. And each trait involved in our experiment probably is very important for F1 morphological phenotypes. Conclusion We conclude that 2n pollen are involved in hybridization and have a competitive advantage while it traversed the stigma and entered into style. The callose plug in the 2n pollen tube was may have strongly influenced the competitive process in R. hybrida . Electronic supplementary material The online version of this article (10.1186/s12870-019-1696-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

The strong competitive role of 2n pollen in several polyploidy hybridizations in Rosa hybrida

et al. 2019

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“…The recent study revealed that 'Nishiuchi Konatsu' 'Kiyomi' tangor H15-701 H15-702 Meiwa kumquat as a bud-mutated cultivar of hyuga-natsu (C. tamurana hort. ex Tanaka) produced the giant pollen derived from the 2n male unreduced gamete by FDR with the single-pollen genotyping method using SSR molecular markers and heterozygous transmission analysis [48,49]. Moreover, Rouiss et al [15] proved the existence of the 2n male unreduced gamete by both FDR and SDR with the maximum-likelihood method based on PHR in tetraploid hybrids from tetraploid−diploid crosses.…”

Section: Discussionmentioning

confidence: 99%

Identification of Parental Genome Construction and Inherited Morphological Characteristics in Triploid and AneuploidIntergeneric Hybrids from a Diploid−Diploid Cross between Citrus and Fortunella

et al. 2021

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We previously obtained two intergeneric hybrids with different ploidies, i.e., aneuploid (2n = 28) and eutriploid, from diploid−diploid crosses between ‘Kiyomi’ tangor (Citrus unshiu Marcow. × C. sinensis (L.) Osbeck) and Meiwa kumquat (Fortunella crassifolia Swingle) as novel breeding materials for a seedless kumquat. In this study, we attempted to clarify the construction of the parental genomes of these hybrids by SSR genotyping and genomic in situ hybridization (GISH)−chromomycin A3 (CMA) analysis. SSR genotyping in NSX43 (LG5) and CiBE2227 (LG8) loci revealed that both hybrids inherited one allele from ‘Kiyomi’ tangor and two heterozygous alleles from Meiwa kumquat. The GISH analysis failed due to the high genomic homology between Citrus and Fortunella. At the same time, the CMA karyotype compositions of the two intergeneric hybrids (H15-701: 2A + 1B + 3C + 13D + 7E + 1F + 1Dst; H15-702: 3A + 1B + 2C + 15D + 4E +1F + 1Dst) and both parents (‘Kiyomi’ tangor: 1A + 2B + 2C + 6D + 7E; Meiwa kumquat: 2A + 2C + 12D + 1F + 1Dst) were completely revealed. We identified the parental genome construction and polyploidization processes in both intergeneric hybrids on the basis of these SSR genotypes and CMA karyotype compositions according to the following theory: the SSR genotypes and chromosome compositions were the same as those of the somatic chromosome and two-fold after the first division (even number) in unreduced gametes caused by first-division restitution (FDR) and second-division restitution (SDR), respectively. Consequently, we determined that both intergeneric hybrids may have had two genomes derived from the 2n male unreduced gamete as a result of the FDR of the Meiwa kumquat. In addition, most horticultural traits of the leaves, flowers, and fruits of both hybrids showed intermediate traits of the parents, but the fruit sizes and flowering habits were more like those of the two inherited genomes of Meiwa kumquat.

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“…After analysing triploid hybrids with clementine as female parent, Luro et al (2004) concluded that 2% arose from 2n pollen. More recently, single-pollen genotyping studies (Honsho et al 2012(Honsho et al , 2016 demonstrated the formation of 2n pollen gametes through FDR mechanism in 'Nishiuchi Konatsu' mandarin (Citrus tamurana Hort. Ex Tanaka), even though the recovery of the plants failed.…”

Section: Introductionmentioning

confidence: 99%

The effect of cross direction and ploidy level on phenotypic variation of reciprocal diploid and triploid mandarin hybrids

Ahmed

Evrard

Ollitrault

et al. 2020

Tree Genetics & Genomes

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New citrus fruit varieties with the right pomological and organoleptic characteristics are expected by consumers and the fresh citrus fruit market. Apart from a good balance between sugar content and acidity, seedlessness is particularly demanded. Triploidy is one of the best ways to obtain seedless cultivars, and, taking advantage of diploid gametes, research programs have succeeded in creating them. Triploid hybrids are sterile and, when associated with parthenocarpy, produce seedless fruits. However, no studies have compared the potential agronomic interest of diploid and triploid cultivars to date. The aims of this study were to investigate the effects of (i) cross direction between diploid and triploid reciprocal populations of mandarin hybrids and (ii) the increase in ploidy level from diploidy to triploidy phenotypic variation in quantitative agronomic traits. Reciprocal crosses between 'Fortune' mandarin and 'Ellendale' tangor generated two diploid and two triploid populations; we then phenotyped by measuring fruit and juice weights, sugar content and titratable acidity over three harvesting campaigns. Significant differences in sugar and acidity levels were observed between triploid populations. Both triploid progenies varied according to their respective female genitor, unlike diploid populations in which no relationship was found between their phenotypic variation and that of their parents. Considering each cross direction separately, the comparison of diploid and triploid populations revealed that triploid hybrids were juicier and sweeter. A genomic dosage effect appears to be the most plausible explanation for these variations. These innovative results will help optimize triploid citrus breeding programmes by choosing the appropriate diploid gamete producing parent.

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Unreduced 2n Pollen Production in ‘Nishiuchi Konatsu’ Hyuganatsu as Inferred by Pollen Characteristics and Progeny Ploidy Level

Cited by 13 publications

References 37 publications

The strong competitive role of 2n pollen in several polyploidy hybridizations in Rosa hybrida

The strong competitive role of 2n pollen in several polyploidy hybridizations in Rosa hybrida

Identification of Parental Genome Construction and Inherited Morphological Characteristics in Triploid and AneuploidIntergeneric Hybrids from a Diploid−Diploid Cross between Citrus and Fortunella

The effect of cross direction and ploidy level on phenotypic variation of reciprocal diploid and triploid mandarin hybrids

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