Unscheduled Dna Synthesis in Frog Lens at 5°c

Abstract

Abstract. Unscheduled DNA labeling occurs in the frog even at low temperatures. We conclude, tentatively, that the UV‐induced labeling observed in these cold incubated lenses represents repair synthesis of DNA.

The Role of DNA damage, its Repair and its Misrepair in the Etiology of Cataract: a Review

“…This additional finding suggests that the initial lesion was repaired during the period of mitotic inhibition. With respect to this, we have determined that organ-cultured lenses from dormant frogs are able to perform DNA repair synthesis at 5°C [23]. Based upon this finding, it is proposed that frogs maintained in a cold environment following ionizing radiation do not develop severe cataracts because the damage to epithelial cell DNA is repaired before the cells can differentiate to form aberrant lens fibers.…”

Unscheduled DNA Repair in Human Lens Epithelium following ‘in vivo’ and ‘in vitro’ Ultraviolet Irradiation

“…She proposed that 'unrepaired or incorrectly repaired damage to lens epithelial cell DNA may result in a decrease of lens transparency either by altering normal metabolism in the epithelium or subsequently by affecting lens cell differentiation and pro tein synthesis in the lens cortex'. She was also the first to demonstrate unscheduled DNA repair following UV irradiation (maximum near 254 nm) in different experimental models (rat and frog lens) [7,8]. To the best of our knowledge our report is the first to describe unscheduled DNA repair following UV-B irradiation in the young, healthy human lens under 'in vivo' and in vitro conditions.…”

“…Under in vitro conditions, repair of UV-induced DNA damage has been shown in rat and frog lenses [7,8]. In these experiments the wavelength used was near 254 nm.…”