We apply the recent frame-invariant theory of separation spike formation to complex unsteady flows including a turbulent separation bubble, an impinging jet, and flows around a freely moving cylinder and a freely rotating ellipse. We show how the theory captures the onset of material spike formation, without any assumption on the flow type (steady, periodic, unsteady) or separation type (on-or off-wall, fixed or moving boundaries). We uncover new phenomena, such as the transition from on-wall to off-wall separation, the merger of initially distinct spikes, and the presence of severe material spikes that remain hidden to previous approaches. These results unveil how an involved network of spikes arises, interacts, and merges dynamically, leading to the final ejection of particles from the wall in highly transient flow separation processes.