Unsupervised Online Classifier in Sleep Scoring for Sleep Deprivation Studies

Libourel, Paul-Antoine; Corneyllie, Alexandra; Luppi, Pierre‐Hervé; Chouvet, Guy; Gervasoni, Damien

doi:10.5665/sleep.4682

Cited by 37 publications

(36 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mice were submitted to CSF using a shaker apparatus (PVC cylinder, diameter 30 cm, height 45 cm; Viewpoint, France) that prevents sleep by transient vertical movements (10–20 ms, height 1 cm) 66 . This system allows sleep disruptions to be achieved without forced locomotion or ambulation.…”

Section: Methodsmentioning

confidence: 99%

Decreased alertness due to sleep loss increases pain sensitivity in mice

Alexandre

Latrémolière

Ferreira

et al. 2017

Nat Med

139

114

View full text Add to dashboard Cite

Extended daytime and nighttime activities are major contributors to the growing sleep deficiency epidemic1,2, as is the high prevalence of sleep disorders like insomnia. The consequences of chronic insufficient sleep for health remain uncertain3. Sleep quality and duration predict presence of pain the next day in healthy subjects4–7, suggesting that sleep disturbances alone may worsen pain, and experimental sleep deprivation in humans supports this claim8,9. We demonstrate that sleep loss, but not sleep fragmentation, in healthy mice increases sensitivity to noxious stimuli (referred to as ‘pain’) without general sensory hyper-responsiveness. Moderate daily repeated sleep loss leads to a progressive accumulation of sleep debt and also to exaggerated pain responses, both of which are rescued after restoration of normal sleep. Caffeine and modafinil, two wake-promoting agents that have no analgesic activity in rested mice, immediately normalize pain sensitivity in sleep-deprived animals, without affecting sleep debt. The reversibility of mild sleep-loss-induced pain by wake-promoting agents reveals an unsuspected role for alertness in setting pain sensitivity. Clinically, insufficient or poor-quality sleep may worsen pain and this enhanced pain may be reduced not by analgesics, whose effectiveness is reduced, but by increasing alertness or providing better sleep.

show abstract

Section: Methodsmentioning

confidence: 99%

Decreased alertness due to sleep loss increases pain sensitivity in mice

Alexandre

Latrémolière

Ferreira

et al. 2017

Nat Med

139

114

View full text Add to dashboard Cite

show abstract

“…We applied the same criteria as used in (Libourel et al, 2015) to evaluate the quality of the 490 agreement : 491…”

Section: Evaluation Of Overlap Of Scoring Methods 480mentioning

confidence: 99%

“…Sleep scoring methods are based on the assumption that the information about sleep states is 49 contained in the recorded signal and can be used as a marker (Libourel et al, 2015). In order to 50 implement a reliable sleep scoring, the candidate marker of sleep and wake must not only show 51 a strong average difference between the two states but this change must be systematic and 52 sustained throughout each state, with a clear separation between the values in each state.…”

Section: Introduction 25mentioning

confidence: 99%

Harnessing olfactory bulb oscillations to perform fully brain-based sleep scoring and track sleep/wake transitions across multiple time scales in mice

Bagur

Lacroix

et al. 2017

Preprint

View full text Add to dashboard Cite

“…However, the expediency and objectivity value of these methods is diminished for those that require a subset of manually scored samples from individual animals to train the algorithm (1)(2)(3)(4). Unsupervised, automated scoring algorithms have addressed these limitations of machine learning with varying degrees of accuracy (72-96% agreement) compared to standard manual scoring (5)(6)(7)(8). Nevertheless, all EEG/EMG based systems of arousal state monitoring are hindered by the time required for surgery, recovery, and in many cases, the repeated-measures experimental designs used for studies in which a small number of animals are used.…”

Section: Introductionmentioning

confidence: 99%

Rapid, noninvasive, and unsupervised detection of sleep/wake using piezoelectric monitoring for pharmacological studies in narcoleptic mice

Black

Sun

Laihsu

et al. 2017

Preprint

View full text Add to dashboard Cite

BackgroundAssessment of sleep/wake by electroencephalography (EEG) and electromyography (EMG) is invasive, resource intensive, and not amenable to rapid screening at scale for drug discovery. In the preclinical development of therapeutics for narcolepsy, efficacy tests are hindered by the lack of a non-EEG/EMG based translational test of symptom severity. The current methods study offers proof-of-principle that PiezoSleep (noninvasive, unsupervised piezoelectric monitoring of gross body movement, together with respiration patterns during behavioral quiescence), can be used to determine sleep/wake as applicable to the development of wake-promoting therapeutics. First, the translational wake-maintenance score (WMS, the ratio of time during the first half of the dark period spent in long wake bouts to short sleep bouts) of the PiezoSleep narcolepsy screen was introduced as a means by which to rank narcoleptic orexin/ataxin-3 mice and wild type mice by sleep/wake fragmentation severity. Accuracy of the WMS to detect narcoleptic phenotypes were determined in genotype-confirmed orexin/ataxin-3 mice and wild type colony mates. The WMS was used to identify the most highly symptomatic mice for resource-intensive EEG/EMG studies for further analysis of specific arousal states. Second, PiezoSleep was demonstrated for use in high-throughput screening of wake-promoting compounds using modafinil in orexin/ataxin-3 and wild type mice.ResultsThe WMS detected a narcoleptic phenotype with 89% sensitivity, 92% specificity and 98% positive predictive value. A 15-fold difference in WMS differentiated wild type littermates from the most severely affected orexin/ataxin-3 mice. Follow-up EEG/EMG study indicated 82% of the orexin/ataxin-3 mice with the lowest wake-maintenance scores met or exceeded the cataplexy-occurrence threshold (≥ 3 bouts) for inclusion in therapeutic efficacy studies. In the PiezoSleep dose-response study, the ED50 for wake-promotion by modafinil was approximately 50 mg/kg in both genotypes. Using unsupervised piezoelectric monitoring, the efficacy of wake-promoting compounds can be determined in a 5-arm study with 60 mice in less than one week—a fraction of the time compared to EEG/EMG studies.ConclusionsThe WMS on the PiezoSleep narcolepsy screen quantifies the inability to sustain wakefulness and provides an accurate measure of the narcoleptic phenotype in mice. PiezoSleep offers rapid, scalable assessment of sleep/wake for high-throughput screening in drug discovery.

show abstract

Unsupervised Online Classifier in Sleep Scoring for Sleep Deprivation Studies

Cited by 37 publications

References 42 publications

Decreased alertness due to sleep loss increases pain sensitivity in mice

Decreased alertness due to sleep loss increases pain sensitivity in mice

Harnessing olfactory bulb oscillations to perform fully brain-based sleep scoring and track sleep/wake transitions across multiple time scales in mice

Rapid, noninvasive, and unsupervised detection of sleep/wake using piezoelectric monitoring for pharmacological studies in narcoleptic mice

Contact Info

Product

Resources

About