Untangling filamentous marine cyanobacterial diversity from the coast of South Florida with the description of Vermifilaceae fam. nov. and three new genera: Leptochromothrix gen. nov., Ophiophycus gen. nov., and Vermifilum gen. nov.

Berthold, David E.; Lefler, Forrest W.; Laughinghouse, Haywood Dail

doi:10.1016/j.ympev.2020.107010

Cited by 20 publications

(18 citation statements)

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“…We have noticed two recent reports published after our phylogenetic trees for the current article were already finished, including the description of new families of marine benthic cyanobacteria, Vermifilaceae and Sirenicapillariaceae (Berthold et al. 2021, 2022). The new families in these studies were based exclusively on 16S rRNA gene data and seemed to be intermixed with Microcoleaceae as defined in our current study (Fig.…”

Section: Resultsmentioning

confidence: 95%

An updated classification of cyanobacterial orders and families based on phylogenomic and polyphasic analysis

Strunecký

Ivanova

Mareš

2023

Journal of Phycology

125

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Cyanobacterial taxonomy is facing a period of rapid changes thanks to the ease of 16S rRNA gene sequencing and established workflows for description of new taxa. Since the last comprehensive review of the cyanobacterial system in 2014 until 2021, at least 273 species in 140 genera were newly described. These taxa were mainly placed into previously defined orders and families although several new families were proposed. However, the classification of most taxa still relied on hierarchical relationships inherited from the classical morphological taxonomy. Similarly, the obviously polyphyletic orders such as Synechococcales and Oscillatoriales were left unchanged. In this study, the rising number of genomic sequences of cyanobacteria and well‐described reference strains allowed us to reconstruct a robust phylogenomic tree for taxonomic purposes. A less robust but better sampled 16S rRNA gene phylogeny was mapped to the phylogenomic backbone. Based on both these phylogenies, a polyphasic classification throughout the whole phylum of Cyanobacteria was created, with ten new orders and fifteen new families. The proposed system of cyanobacterial orders and families relied on a phylogenomic tree but still employed phenotypic apomorphies where possible to make it useful for professionals in the field. It was, however, confirmed that morphological convergence of phylogenetically distant taxa was a frequent phenomenon in cyanobacteria. Moreover, the limited phylogenetic informativeness of the 16S rRNA gene, resulting in ambiguous phylogenies above the genus level, emphasized the integration of genomic data as a prerequisite for the conclusive taxonomic placement of a vast number of cyanobacterial genera in the future.

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Section: Resultsmentioning

confidence: 95%

An updated classification of cyanobacterial orders and families based on phylogenomic and polyphasic analysis

Strunecký

Ivanova

Mareš

2023

Journal of Phycology

125

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“…The cyanobacterium was classified into L. valpauliae by morphological observation using a microscope and phylogenetic analysis of its 16S rDNA sequence . Its EtOH extract was filtered, concentrated, and partitioned between EtOAc and H 2 O.…”

Section: Resultsmentioning

confidence: 99%

Structural Determination, Total Synthesis, and Biological Activity of Iezoside, a Highly Potent Ca²⁺-ATPase Inhibitor from the Marine Cyanobacterium Leptochromothrix valpauliae

et al. 2022

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Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) is a membrane protein on the endoplasmic reticulum (ER) that transports Ca2+ from the cytosol into the ER. As its function is associated with various biological phenomena, SERCA has been recognized as a promising druggable target. Here, we report the second-strongest SERCA-inhibitory compound known to date, which we isolated from the marine cyanobacterium Leptochromothrix valpauliae and named iezoside (1). The structure of iezoside (1) is fundamentally different from that of any other SERCA inhibitor, and its potency is the strongest among marine natural products (K i 7.1 nM). In this article, we report our comprehensive analysis of iezoside (1), which covers its isolation, structural characterization supported by density functional theory (DFT) calculations and statistical analysis, total synthesis, and clarification of the mode of action of its potent antiproliferative activity (IC50 6.7 ± 0.4 nM against HeLa cells).

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“…Microcystis is prevalent in Florida freshwaters (Williams et al, 2007;Burns, 2008) although toxicity levels are highly variable (Lefler et al, 2020). It forms extensive, reoccurring blooms in the diverse freshwater environments throughout the state, including the St. Johns River (Williams et al, 2007) and Lake Okeechobee (Ramani et al, 2012;Kramer et al, 2018) where blooms are released through water management practices into its tributaries, the St. Lucie (Oehrle et al, 2017;Kramer et al, 2018) and Caloosahatchee (Urakawa et al, 2020;Metcalf et al, 2021) Rivers.…”

Section: Microcystis Speciesmentioning

confidence: 99%

“…They are also more resistant to chemical controls (Phlips et al, 1992;Dubose et al, 1997; see review by Wood et al, 2020). Their taxonomy is currently under extensive revision (Berthold et al, 2020;Lefler et al, 2021) with multiple new genera descriptions (e.g., Affixifilum, Neolyngbya, Leptochromothrix, Vermifilum, and Ophiophycus). Recent genetic analysis has also split the genus Lyngbya into new genera [e.g., Moorena (Engene et al, 2012), Limnoraphis (Komárek et al, 2013), Okeania (Engene et al, 2013), Microseira (McGregor andSendall, 2015), and Dapis (Engene et al, 2018)] so it is likely that the current nomenclature of Lyngbya and Microseira species identified from Florida fresh and marine waters will be revised.…”

Section: Benthic and Epiphytic Cyanobacterial Proliferationsmentioning

confidence: 99%

Florida’s Harmful Algal Bloom (HAB) Problem: Escalating Risks to Human, Environmental and Economic Health With Climate Change

Heil

Muni-Morgan

2021

Front. Ecol. Evol.

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Harmful Algal Blooms (HABs) pose unique risks to the citizens, stakeholders, visitors, environment and economy of the state of Florida. Florida has been historically subjected to reoccurring blooms of the toxic marine dinoflagellate Karenia brevis (C. C. Davis) G. Hansen & Moestrup since at least first contact with explorers in the 1500’s. However, ongoing immigration of more than 100,000 people year–1 into the state, elevated population densities in coastal areas with attendant rapid, often unregulated development, coastal eutrophication, and climate change impacts (e.g., increasing hurricane severity, increases in water temperature, ocean acidification and sea level rise) has likely increased the occurrence of other HABs, both freshwater and marine, within the state as well as the number of people impacted by these blooms. Currently, over 75 freshwater, estuarine, coastal and marine HAB species are routinely monitored by state agencies. While only blooms of K. brevis, the dinoflagellate Pyrodinium bahamense (Böhm) Steidinger, Tester, and Taylor and the diatom Pseudo-nitzschia spp. have resulted in closure of commercial shellfish beds, other HAB species, including freshwater and marine cyanobacteria, pose either imminent or unknown risks to human, environmental and economic health. HAB related human health risks can be classified into those related to consumption of contaminated shellfish and finfish, consumption of or contact with bloom or toxin contaminated water or exposure to aerosolized HAB toxins. While acute human illnesses resulting from consumption of brevetoxin-, saxitoxin-, and domoic acid-contaminated commercial shellfish have been minimized by effective monitoring and regulation, illnesses due to unregulated toxin exposures, e.g., ciguatoxins and cyanotoxins, are not well documented or understood. Aerosolized HAB toxins potentially impact the largest number of people within Florida. While short-term (days to weeks) impacts of aerosolized brevetoxin exposure are well documented (e.g., decreased respiratory function for at-risk subgroups such as asthmatics), little is known of longer term (>1 month) impacts of exposure or the risks posed by aerosolized cyanotoxin [e.g., microcystin, β-N-methylamino-L-alanine (BMAA)] exposure. Environmental risks of K. brevis blooms are the best studied of Florida HABs and include acute exposure impacts such as significant dies-offs of fish, marine mammals, seabirds and turtles, as well as negative impacts on larval and juvenile stages of many biota. When K. brevis blooms are present, brevetoxins can be found throughout the water column and are widespread in both pelagic and benthic biota. The presence of brevetoxins in living tissue of both fish and marine mammals suggests that food web transfer of these toxins is occurring, resulting in toxin transport beyond the spatial and temporal range of the bloom such that impacts of these toxins may occur in areas not regularly subjected to blooms. Climate change impacts, including temperature effects on cell metabolism, shifting ocean circulation patterns and changes in HAB species range and bloom duration, may exacerbate these dynamics. Secondary HAB related environmental impacts are also possible due to hypoxia and anoxia resulting from elevated bloom biomass and/or the decomposition of HAB related mortalities. Economic risks related to HABs in Florida are diverse and impact multiple stakeholder groups. Direct costs related to human health impacts (e.g., increased hospital visits) as well as recreational and commercial fisheries can be significant, especially with wide-spread sustained HABs. Recreational and tourism-based industries which sustain a significant portion of Florida’s economy are especially vulnerable to both direct (e.g., declines in coastal hotel occupancy rates and restaurant and recreational users) and indirect (e.g., negative publicity impacts, associated job losses) impacts from HABs. While risks related to K. brevis blooms are established, Florida also remains susceptible to future HABs due to large scale freshwater management practices, degrading water quality, potential transport of HABs between freshwater and marine systems and the state’s vulnerability to climate change impacts.

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Untangling filamentous marine cyanobacterial diversity from the coast of South Florida with the description of Vermifilaceae fam. nov. and three new genera: Leptochromothrix gen. nov., Ophiophycus gen. nov., and Vermifilum gen. nov.

Cited by 20 publications

References 60 publications

An updated classification of cyanobacterial orders and families based on phylogenomic and polyphasic analysis

An updated classification of cyanobacterial orders and families based on phylogenomic and polyphasic analysis

Structural Determination, Total Synthesis, and Biological Activity of Iezoside, a Highly Potent Ca²⁺-ATPase Inhibitor from the Marine Cyanobacterium Leptochromothrix valpauliae

Florida’s Harmful Algal Bloom (HAB) Problem: Escalating Risks to Human, Environmental and Economic Health With Climate Change

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Untangling filamentous marine cyanobacterial diversity from the coast of South Florida with the description of Vermifilaceae fam. nov. and three new genera: Leptochromothrix gen. nov., Ophiophycus gen. nov., and Vermifilum gen. nov.

Cited by 20 publications

References 60 publications

An updated classification of cyanobacterial orders and families based on phylogenomic and polyphasic analysis

An updated classification of cyanobacterial orders and families based on phylogenomic and polyphasic analysis

Structural Determination, Total Synthesis, and Biological Activity of Iezoside, a Highly Potent Ca2+-ATPase Inhibitor from the Marine Cyanobacterium Leptochromothrix valpauliae

Florida’s Harmful Algal Bloom (HAB) Problem: Escalating Risks to Human, Environmental and Economic Health With Climate Change

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Structural Determination, Total Synthesis, and Biological Activity of Iezoside, a Highly Potent Ca²⁺-ATPase Inhibitor from the Marine Cyanobacterium Leptochromothrix valpauliae