Untapped talents: insight into the ecological significance of methanotrophs and its prospects

Fenibo, Emmanuel O.; Selvarajan, Ramganesh; Wang, Huiqi; Wang, Yue; Abia, Akebe Luther King

doi:10.1016/j.scitotenv.2023.166145

Cited by 8 publications

(1 citation statement)

References 189 publications

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“…According to different environmental characteristics and formation, the deep sea can be roughly divided into hydrothermal vents, cold seeps, seamounts, hadal trenches, mid-ocean ridges, and other habitats (Bian et al, 2010 ; Orcutt et al, 2011 ). Microorganisms are at the base of the food web for deep-ocean organisms and drive abyssal biogeochemical cycles (Choy et al, 2017 ; Fenibo et al, 2023 ). Microbes, such as bacteria which are susceptible to phage infection, have been increasingly recognized as major ecosystem players because they are abundant and infect organisms that form the basis of ocean biogeochemical cycling (Suttle, 2007 ; Steward et al, 2013 ; Moniruzzaman et al, 2017 ).…”

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confidence: 99%

Diversities and interactions of phages and bacteria in deep-sea sediments as revealed by metagenomics

Sun,

Jiang,

Zhang

2024

Front. Microbiol.

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Phages are found virtually everywhere, even in extreme environments, and are extremely diverse both in their virion structures and in their genomic content. They are thought to shape the taxonomic and functional composition of microbial communities as well as their stability. A number of studies on laboratory culture and viral metagenomic research provide deeper insights into the abundance, diversity, distribution, and interaction with hosts of phages across a wide range of ecosystems. Although most of these studies focus on easily accessible samples, such as soils, lakes, and shallow oceans, little is known about bathypelagic phages. In this study, through analyzing the 16S rRNA sequencing and viral metagenomic sequencing data of 25 samples collected from five different bathypelagic ecosystems, we detected a high diversity of bacteria and phages, particularly in the cold seep and hydrothermal vent ecosystems, which have stable chemical energy. The relative abundance of phages in these ecosystems was higher than in other three abyssal ecosystems. The low phage/host ratios obtained from host prediction were different from shallow ecosystems and indicated the prevalence of prophages, suggesting the complexity of phage–bacteria interactions in abyssal ecosystems. In the correlation analysis, we revealed several phages–bacteria interaction networks of potential ecological relevance. Our study contributes to a better understanding of the interactions between bathypelagic bacteria and their phages.

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Section: Introductionmentioning

confidence: 99%

Diversities and interactions of phages and bacteria in deep-sea sediments as revealed by metagenomics

Sun,

Jiang,

Zhang

2024

Front. Microbiol.

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Characterization and identification of long-chain hydrocarbon-degrading bacterial communities in long-term chronically polluted soil in Ogoniland: an integrated approach using culture-dependent and independent methods

Okoye,

Selvarajan,

Chikere

et al. 2024

Environ Sci Pollut Res

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Escalating oil consumption has resulted in an increase in accidental spills of petroleum hydrocarbons, causing severe environmental degradation, notably in vulnerable regions like the Niger Delta. Complex mixture of these hydrocarbons particularly long-chain alkanes presents unique challenges in restoration of polluted environment due to their chemical properties. This study aimed to investigate the long-chain hydrocarbon-degrading bacterial communities within long-term chronically polluted soil in Ogoniland, by utilizing both traditional cultivation methods and modern culture-independent techniques. Results revealed that surface-polluted soil (SPS) and subsurface soil (SPSS) exhibit significantly higher total organic carbon (TOC) ranging from 5.64 to 5.06% and total petroleum hydrocarbons (TPH) levels ranging from 36,775 ppm to 14,087 ppm, compared to unpolluted soil (UPS) with 1.97% TOC and 479 ppm TPH, respectively. Analysis of carbon chain lengths reveals the prevalence of longer-chain alkanes (C20-28) in the surface soil. Culture-dependent methods, utilizing crude oil enrichment (COE) and paraffin wax enrichment (PWE), yield 47 bacterial isolates subjected to a long-chain alkane degradation assay. Twelve bacterial strains demonstrate significant degradation abilities across all enriched media. Three bacterial members, namely Pseudomonas sp. (almA), Marinomonas sp. (almA), and Alteromonas (ladA), exhibit genes responsible for long-chain alkane degradation, demonstrating efficiency between 50 and 80%. Culture-independent analysis reveals that surface SPS samples exhibit greater species richness and diversity compared to subsurface SPSS samples. Proteobacteria dominates as the phylum in both soil sample types, ranging from 22.23 to 82.61%, with Firmicutes (0.2–2.22%), Actinobacteria (0.4–3.02%), and Acidobacteria (0.1–3.53%) also prevalent. Bacterial profiles at genus level revealed that distinct variations among bacterial populations between SPS and SPSS samples comprising number of hydrocarbon degraders and the functional predictions also highlight the presence of potential catabolic genes (nahAa, adh2, and cpnA) in the polluted soil. However, culture-dependent analysis only captured a few of the dominant members found in culture-independent analysis, implying that more specialized media or environments are needed to isolate more bacterial members. The findings from this study contribute valuable information to ecological and biotechnological aspects, aiding in the development of more effective bioremediation applications for restoring oil-contaminated environments.

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Biologic Conversion of Methane to Methanol

de Moraes Ferreira,

Resende,

Ribeiro

et al. 2024

Reference Module in Chemistry, Molecular Sciences and Chemical Engineering

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Untapped talents: insight into the ecological significance of methanotrophs and its prospects

Cited by 8 publications

References 189 publications

Diversities and interactions of phages and bacteria in deep-sea sediments as revealed by metagenomics

Diversities and interactions of phages and bacteria in deep-sea sediments as revealed by metagenomics

Characterization and identification of long-chain hydrocarbon-degrading bacterial communities in long-term chronically polluted soil in Ogoniland: an integrated approach using culture-dependent and independent methods

Biologic Conversion of Methane to Methanol

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