2024
DOI: 10.1101/2024.09.16.613180
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Untargeted and Semi-Targeted Metabolomics Approach for Profiling Small Intestinal and Fecal Metabolome Using High-Resolution Mass Spectrometry

Alexandre Tronel,
Morgane Roger-Margueritat,
Caroline Plazy
et al.

Abstract: The gut microbiome is a complex ecosystem varying along different gut sections, consisting of metabolites from food, host, and microbes. Microbially derived metabolites like bile acids and short chain fatty acids interact with host physiology. Current studies often use fecal samples, which don′t fully represent the upper gut due to stratification. To sample the proximal gut microbiome, endoscopic methods or new non invasive devices are used. We developed an approach combining untargeted and semi−targeted metab… Show more

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