Untargeted LC-QTOF-MS/MS-based metabolomics of spent coffee grounds alcoholic beverages fermented with Lachancea thermotolerans and Lactiplantibacillus plantarum

Liu, Yunjiao; Lu, Yuyun; Liu, Shao-Quan

doi:10.1016/j.foodres.2023.112733

Cited by 10 publications

(3 citation statements)

References 49 publications

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“…4 ) ( Mok et al, 2019 ). Besides this metabolic pathway, LAB can also degrade malic acid into lactic acid through malolactic fermentation ( Liu et al, 2023 ). There was a slight increase of lactic acid in both PCC- and LAC-fermented samples (1.3-fold and 1.2-fold increase respectively) ( Table S2 ).…”

Section: Resultsmentioning

confidence: 99%

Metabolomics analysis of okara probiotic beverages fermented with Lactobacillus gasseri and Limosilactobacillus fermentum by LC-QTOF-MS/MS

Gao,

Zhou,

Lin

et al. 2024

Food Chemistry: X

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Section: Resultsmentioning

confidence: 99%

Metabolomics analysis of okara probiotic beverages fermented with Lactobacillus gasseri and Limosilactobacillus fermentum by LC-QTOF-MS/MS

Gao,

Zhou,

Lin

et al. 2024

Food Chemistry: X

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“…Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (LC-QTOF-MS) is recognized as a non-targeted metabolomics analytical method capable of analyzing extracellular and intracellular metabolites. Owing to its broad dynamic range and high sensitivity, it is employed as an effective tool for characterizing a wide array of metabolites [31].…”

Section: Introductionmentioning

confidence: 99%

“…2023, 24, x FOR PEER REVIEW 3 of 14 extracellular and intracellular metabolites. Owing to its broad dynamic range and high sensitivity, it is employed as an effective tool for characterizing a wide array of metabolites [31]. Previously, it has been extensively applied in various fields, such as observing metabolite variations in fermented food and beverages, identifying bioactive compounds, and conducting disease analysis [32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Revealing the Mechanisms of Enhanced β-Farnesene Production in Yarrowia lipolytica through Metabolomics Analysis

Liu,

Bi,

Wang

et al. 2023

IJMS

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β-Farnesene is an advanced molecule with promising applications in agriculture, the cosmetics industry, pharmaceuticals, and bioenergy. To supplement the shortcomings of rational design in the development of high-producing β-farnesene strains, a Metabolic Pathway Design-Fermentation Test-Metabolomic Analysis-Target Mining experimental cycle was designed. In this study, by over-adding 20 different amino acids/nucleobases to induce fluctuations in the production of β-farnesene, the changes in intracellular metabolites in the β-farnesene titer-increased group were analyzed using non-targeted metabolomics. Differential metabolites that were detected in each experimental group were selected, and their metabolic pathways were located. Based on these differential metabolites, targeted strain gene editing and culture medium optimization were performed. The overexpression of the coenzyme A synthesis-related gene pantothenate kinase (PanK) and the addition of four mixed water-soluble vitamins in the culture medium increased the β-farnesene titer in the shake flask to 1054.8 mg/L, a 48.5% increase from the initial strain. In the subsequent fed-batch fermentation, the β-farnesene titer further reached 24.6 g/L. This work demonstrates the tremendous application value of metabolomics analysis for the development of industrial recombinant strains and the optimization of fermentation conditions.

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Highlights for the advanced analysis of bioactive compounds in coffee

Pua,

Goh,

Huang

et al. 2025

Coffee in Health and Disease Prevention

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Untargeted LC-QTOF-MS/MS-based metabolomics of spent coffee grounds alcoholic beverages fermented with Lachancea thermotolerans and Lactiplantibacillus plantarum

Cited by 10 publications

References 49 publications

Metabolomics analysis of okara probiotic beverages fermented with Lactobacillus gasseri and Limosilactobacillus fermentum by LC-QTOF-MS/MS

Metabolomics analysis of okara probiotic beverages fermented with Lactobacillus gasseri and Limosilactobacillus fermentum by LC-QTOF-MS/MS

Revealing the Mechanisms of Enhanced β-Farnesene Production in Yarrowia lipolytica through Metabolomics Analysis

Highlights for the advanced analysis of bioactive compounds in coffee

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