Untargeted liquid chromatography coupled with mass spectrometry reveals metabolic changes in nitrogen-deficient Isatis indigotica Fortune

Cao, Yiwen; Qu, Renjun; Miao, Yujing; Tang, Xiao‐Qing; Zhou, Ying; Wang, Lei; Geng, Li

doi:10.1016/j.phytochem.2019.112058

Cited by 20 publications

(14 citation statements)

References 52 publications

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“…Liquid chromatography tandem mass spectrometry analysis. LC-MS/MS analyses were performed according to previous report 27 . In brief, the UHPLC system (1290, Agilent Technologies) with a UPLC BEH Amide column (1.7 μm 2.1*100 mm, Waters) coupled to TripleTOF 6600 (Q-TOF, AB Sciex) was used.…”

Section: Animals and Acclimatizationmentioning

confidence: 99%

“…Data preprocessing and annotation. According to previous report 27 , MS raw data files were converted to the mzXML format using ProteoWizard 28 , and processed by R 29 package XCMS (version 3.2) 30 . The preprocessing results generated a data matrix that consisted of the retention time, mass-to-charge ratio (m/z) values, and peak intensity.…”

Section: Animals and Acclimatizationmentioning

confidence: 99%

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Metabolomics Adaptation of Juvenile Pacific Abalone Haliotis discus hannai to Heat Stress

Gao

Liu

2020

Sci Rep

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Temperature fluctuation is a key abiotic factor for the growth and survival of Pacific abalone Haliotis discus hannai, particularly during climate change. However, the physiological mechanism underlying the abalones' response to heat stress remains unknown. We sought to understand the metabolic adaptation mechanism of Pacific abalone to heat stress for further analyzing its heat tolerance capacity. For two groups experienced different acclimate temperature (10 °C and 30 °C for 62 days), the Pacific abalone juveniles displayed significantly different survival rates under 31 °C acute heat treatment. A total of 1815 and 1314 differential metabolites were identified from the 10 °C and 30 °C acclimate groups respectively, by comparing mass spectrometry data of the samples before and after heat stimulation. Heat stress led to mitochondrial failure, resulting in incomplete oxidative metabolism of amino acids and fatty acids in the mitochondria, and massive accumulation of unstable metabolic intermediates in cells. The 10 °C acclimated group accumulated more harmful substances after heat stimulation, provoking further stress responses and pathophysiological processes. In comparison, the 30 °C acclimated group showed stronger regulation capacity to produce beneficial substances for metabolic homeostasis. The findings provided insight into the heat response of marine animals, especially concerning mitochondrial metabolism. Global warming will trigger more extreme weather events that can potentially be deleterious to marine animals, especially the ones distributed in the intertidal and neritic zones 1,2. Organisms inhabiting this range face higher water temperature elevations and more environmental fluctuations during the summer season. Aquaculture animals, which are subjected to fluctuations in natural conditions, are more vulnerable to these physical and chemical stimulations as they are usually stocked intensively at high density and rely on water influx from coastal areas 3. Even a slight change in the climate may cause enormous economic losses in mariculture. The analysis of thermal stress on aquaculture animals has thus gained more research interests 4-7. Pacific abalone Haliotis discus hannai, a cold water gastropod, is the predominantly farmed abalone species in many Asian countries 8. It is naturally distributed in cold water, implying that high temperatures would limit its southern expansion along the Asian coast. In recent years, the dominant aquaculture strategy in China is transferring juvenile abalones to warmer south China during the winter, thereby accelerating the animal's growth rate. In the meantime, the traditional genetic selection based on growth traits may contribute to a decreased heat stress tolerance of the organism. Consequently, the massive mortality in summer months has become a challenge that has led to an enormous economic loss in abalone and the entire molluscs industry in recent years 9. While some overwintering abalones were transferred back to the northern region during spring, some were c...

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Section: Animals and Acclimatizationmentioning

confidence: 99%

Section: Animals and Acclimatizationmentioning

confidence: 99%

Metabolomics Adaptation of Juvenile Pacific Abalone Haliotis discus hannai to Heat Stress

Gao

Liu

2020

Sci Rep

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“…The secondary metabolites in plants are numerous; by ultra-high-performance liquid chromatography, a total of 132 metabolites in Scutellaria baicalensis, 447 metabolites in Isatis indigotica Fortune, 122 in Moringa oleifera leaves, and 128 in American ginseng roots were identified [8][9][10][11], most of which were secondary metabolites. It has been proven that the proportion of secondary metabolites varies according to ever-changing environmental conditions [12].…”

Section: Introductionmentioning

confidence: 99%

Complexity of active medicinal ingredients in radix scutellariae with sodium hydrosulfite exposure

et al. 2020

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Both plants and animals are living things made up of similar cells as well as organelles, and their essence of life is the same. However, plants face more environmental stress than animals and generate excessive reactive oxygen species (ROS), a group of small molecules that can harm proteins, necessitating distinctive metabolic processes. Secondary metabolites in plants are a group of chemical components that can eliminate ROS and can also exhibit medicinal properties; therefore, herbal medicines are often closely linked to the ecological significance of secondary metabolites. Why plants contain so many, not few, active medicinal ingredients is unknown. The root of Scutellaria baicalensis, a popular herbal medicine, is rich in various flavonoids with diverse structural features. Sodium hydrosulfite (Na 2 S 2 O 4) can produce O_-2 radicals and induce physical conditions under environmental stress. Using UHPLC-ESI-Q-TOF-MS/MS analysis, a total of 25 different compounds were identified in the roots of S. baicalensis between the Na 2 S 2 O 4 groups and suitable conditions. Based on the results of the t-test (P<0.05) performed for the groups and ions with values of VIP � 2, the most significantly different chemical markers with Na 2 S 2 O 4 treatment were shi

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“…We did not observe a significant change in indole-3-acetate (auxin) levels upon endosidin-7 treatment; however, 5-hydroxyindole-3-acetate showed a significant increase in roots ( Fig 4F ). In independent plant metabolomics studies in melon, soybean, and Isatis indigotica [ 90 , 94 , 95 ], 5-hydroxyindole-3-acetate is reported to be putatively synthesized from tryptophan and it is speculated to affect auxin metabolism in plants [ 90 , 95 ]. Considering the increase of xanthine and 1,7-dimethylxanthine in roots ( Figs 3 and 4G ), it is likely that downstream of the purine metabolism, cytokinins synthesized from the deoxyxylulose pathway [ 96 , 97 ] are affected upon endosidin-7 treatment.…”

Section: Discussionmentioning

confidence: 99%

NMR spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor

et al. 2020

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In plant cytokinesis, de novo formation of a cell plate evolving into the new cell wall partitions the cytoplasm of the dividing cell. In our earlier chemical genomics studies, we identified and characterized the small molecule endosidin-7, that specifically inhibits callose deposition at the cell plate, arresting late-stage cytokinesis in arabidopsis. Endosidin-7 has emerged as a very valuable tool for dissecting this essential plant process. To gain insights regarding its mode of action and the effects of cytokinesis inhibition on the overall plant response, we investigated the effect of endosidin-7 through a nuclear magnetic resonance spectroscopy (NMR) metabolomics approach. In this case study, metabolomics profiles of arabidopsis leaf and root tissues were analyzed at different growth stages and endosidin-7 exposure levels. The results show leaf and root-specific metabolic profile changes and the effects of endosidin-7 treatment on these metabolomes. Statistical analyses indicated that the effect of endosidin-7 treatment was more significant than the developmental impact. The endosidin-7 induced metabolic profiles suggest compensations for cytokinesis inhibition in central metabolism pathways. This study further shows that long-term treatment of endosidin-7 profoundly changes, likely via alteration of hormonal regulation, the primary metabolism of arabidopsis seedlings. Hormonal pathway-changes are likely reflecting the plant’s responses, compensating for the arrested cell division, which in turn are leading to global metabolite modulation. The presented NMR spectral data are made available through the Metabolomics Workbench, providing a reference resource for the scientific community.

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Untargeted liquid chromatography coupled with mass spectrometry reveals metabolic changes in nitrogen-deficient Isatis indigotica Fortune

Cited by 20 publications

References 52 publications

Metabolomics Adaptation of Juvenile Pacific Abalone Haliotis discus hannai to Heat Stress

Metabolomics Adaptation of Juvenile Pacific Abalone Haliotis discus hannai to Heat Stress

Complexity of active medicinal ingredients in radix scutellariae with sodium hydrosulfite exposure

NMR spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor

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