Untargeted metabolomics in halophytes: The role of different metabolites in New Zealand mangroves under multi-factorial abiotic stress conditions

Ravi, Sridevi; Young, Tim; Macinnis‐Ng, Cate; Nyugen, Thao V.; Duxbury, Mark; Leuzinger, Sebastian

doi:10.1016/j.envexpbot.2020.103993

Cited by 27 publications

(11 citation statements)

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“…Pathway enrichment analysis identified the most affected metabolic pathways in peanut plants under individual and combined abiotic stresses. Salt, drought, and salt-drought stress differentially accumulated free amino acids in mangrove ( Avicennia marina ) [ 59 ]. Likewise, individual and combined abiotic stresses significantly affected amino acids, especially those in the valine, leucine, and isoleucine biosynthesis pathways.…”

Section: Discussionmentioning

confidence: 99%

Differential Physio-Biochemical and Metabolic Responses of Peanut (Arachis hypogaea L.) under Multiple Abiotic Stress Conditions

Patel

Khandwal

Choudhary

et al. 2022

IJMS

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The frequency and severity of extreme climatic conditions such as drought, salinity, cold, and heat are increasing due to climate change. Moreover, in the field, plants are affected by multiple abiotic stresses simultaneously or sequentially. Thus, it is imperative to compare the effects of stress combinations on crop plants relative to individual stresses. This study investigated the differential regulation of physio-biochemical and metabolomics parameters in peanut (Arachis hypogaea L.) under individual (salt, drought, cold, and heat) and combined stress treatments using multivariate correlation analysis. The results showed that combined heat, salt, and drought stress compounds the stress effect of individual stresses. Combined stresses that included heat had the highest electrolyte leakage and lowest relative water content. Lipid peroxidation and chlorophyll contents did not significantly change under combined stresses. Biochemical parameters, such as free amino acids, polyphenol, starch, and sugars, significantly changed under combined stresses compared to individual stresses. Free amino acids increased under combined stresses that included heat; starch, sugars, and polyphenols increased under combined stresses that included drought; proline concentration increased under combined stresses that included salt. Metabolomics data that were obtained under different individual and combined stresses can be used to identify molecular phenotypes that are involved in the acclimation response of plants under changing abiotic stress conditions. Peanut metabolomics identified 160 metabolites, including amino acids, sugars, sugar alcohols, organic acids, fatty acids, sugar acids, and other organic compounds. Pathway enrichment analysis revealed that abiotic stresses significantly affected amino acid, amino sugar, and sugar metabolism. The stress treatments affected the metabolites that were associated with the tricarboxylic acid (TCA) and urea cycles and associated amino acid biosynthesis pathway intermediates. Principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and heatmap analysis identified potential marker metabolites (pinitol, malic acid, and xylopyranose) that were associated with abiotic stress combinations, which could be used in breeding efforts to develop peanut cultivars that are resilient to climate change. The study will also facilitate researchers to explore different stress indicators to identify resistant cultivars for future crop improvement programs.

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Section: Discussionmentioning

confidence: 99%

Differential Physio-Biochemical and Metabolic Responses of Peanut (Arachis hypogaea L.) under Multiple Abiotic Stress Conditions

Patel

Khandwal

Choudhary

et al. 2022

IJMS

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“…In general, starch is mobilised after converting to simple soluble sugars, which are the source of required metabolic energy and exists in the course of ATP production for various phyto-processes (Huner et al 1998;de Block et al 2005;Sulpice et al 2009). Primary metabolites like glucose, sucrose, fructose and starch are known as non-structural carbohydrates (NSCs), which represent the bulk of plant carbon (Ravi et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Stress-induced carbon starvation in Rhizophora mucronata Lam. seedlings under conditions of prolonged submergence and water deficiency: Survive or succumb

et al. 2020

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The behaviour of carbohydrate metabolism in a plant, particularly its total starch content, total soluble sugar (TSS) content and their utilisation, is of great importance in coping with abiotic stress conditions. With this in mind, we studied total starch and TSS contents, survival, growth, biomass accumulation and stomatal conductance in Rhizophora mucronata under conditions of prolonged submergence and water stress for a period of 11 months. The experiment was designed in such a way as to include three replicates per each treatment level, about 1600 young mangrove plants being subjected to study in the process. Under conditions of prolonged submergence and high levels of water stress, a small number of mangrove plants survived and they were promptly exhausted due to higher starch utilisation rates (0.75-1.05% dry mass/month). Although TSS content was increased under these intense stress conditions, it was not matched by increased seedling growth or biomass production; instead, a significant reduction in growth (i.e., ~78%) and dry matter content was observed in stressed seedlings as compared to young plants in the respective controls. It follows that the intense increase of TSS content might be due to the direct conversion of starch to soluble sugars in order to produce metabolic energy for tolerance mechanisms like osmoregulation and root anatomical adaptations under stress conditions. This indicates that more energy is allocated for plant maintenance than for growth and biomass production under stress conditions, which might be a good acclimatory strategy to rescue young mangrove plants at the early phase. However, stomatal closure under stress conditions may have caused restricted photosynthesis. Therefore, stress-induced starch degradation may upsurge, which in turn might lead in the long-run to carbon starvation, a condition lethal to mangrove seedlings.

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“…Salicylic acid is acting as an important plant hormone and assumes a wide range of functions in stress signalling and tolerance to salinity, especially regarding the K + versus Na + discrimination, regulation of Na + long-distance transport, H + -ATPase activity involved in toxic ion compartmentation and the prevention of stress-induced K + leakage [ 26 ]. Sinapic acid offers efficient potential to manage protection against salt-induced reactive oxygen species, as recently demonstrated for the mangrove species Avicenia marina [ 27 ]. Moreover, p -coumaric acid produced by phenylpropanoid pathway may be activated by Coenzyme A to produce monolignols acting as a precursor for lignin synthesis and plays a crucial role in cell wall remodelling under salt stress, allowing one to regulate cell wall extensibility and thus cell elongation, despite turgor modifications in stressed plants [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Transgenerational Effects of Salt Stress Imposed to Rapeseed (Brassica napus var. oleifera Del.) Plants Involve Greater Phenolic Content and Antioxidant Activity in the Edible Sprouts Obtained from Offspring Seeds

Benincasa

Bravi

Marconi

et al. 2021

Plants

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Previous research has demonstrated that rapeseed sprouts obtained under salinity demonstrate greater phenolic content and antioxidant activity compared to those sprouted with distilled water. This work aimed to test the hypothesis that these effects of salinity may persist into the next generation, so that offspring seeds of plants grown under salt stress may give edible sprouts with increased phenolic content and antioxidant activity. Plants of one rapeseed cultivar were grown in pots with 0, 100 and 200 mM NaCl, isolated from each other at flowering to prevent cross-pollination. Offspring seeds harvested from each salinity treatment were then sprouted with distilled water. We performed the extraction of free and bound phenolic fractions of sprouts and, in each fraction (methanolic extract), we determined the total polyphenols (P), flavonoids, (F), and tannins (T) with Folin–Ciocalteu reagent, the phenolic acids (PAs) by ultra-high-performance liquid chromatographs (UHPLC) analysis, and the antioxidant activity with three tests (2,2-diphenyl-1-picrylhydrazyl-hydrate, DPPH; ferric reducing antioxidant power, FRAP; 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] diammonium salt, ABTS). Individual seed weight was slightly decreased by salinity, whereas germination performance was improved, with a lower mean germination time for salted treatments. No significant differences were observed among treatments for P, F and T, except for bound P, while, in most cases, single PAs (as free, bound and total fractions) and antioxidant activity were significantly increased in salted treatments. Our results open new perspectives for the elicitation of secondary metabolites in the offspring seeds by growing parental plants under stressing conditions, imposed on purpose or naturally occurring.

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Untargeted metabolomics in halophytes: The role of different metabolites in New Zealand mangroves under multi-factorial abiotic stress conditions

Cited by 27 publications

References 50 publications

Differential Physio-Biochemical and Metabolic Responses of Peanut (Arachis hypogaea L.) under Multiple Abiotic Stress Conditions

Differential Physio-Biochemical and Metabolic Responses of Peanut (Arachis hypogaea L.) under Multiple Abiotic Stress Conditions

Stress-induced carbon starvation in Rhizophora mucronata Lam. seedlings under conditions of prolonged submergence and water deficiency: Survive or succumb

Transgenerational Effects of Salt Stress Imposed to Rapeseed (Brassica napus var. oleifera Del.) Plants Involve Greater Phenolic Content and Antioxidant Activity in the Edible Sprouts Obtained from Offspring Seeds

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