Untargeted Metabolomics Reveals a Multi-Faceted Resistance Response to Fusarium Head Blight Mediated by the Thinopyrum elongatum Fhb7E Locus Transferred via Chromosome Engineering into Wheat

Fanelli, Guido; Kuzmanović, Ljiljana; Giovenali, Gloria; Tundo, Silvio; Mandalà, Giulia; Rinalducci, Sara; Ceoloni, Carla

doi:10.3390/cells12081113

Cited by 3 publications

(1 citation statement)

References 142 publications

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“…To overcome this narrow genetic variation in durum wheat, many studies have been conducted to introgress resistance from wild or cultivated relatives to durum what, e.g. T. dicoccoides , T. dicoccum, T. elongatum and T. carthlicum [ 13 , 18 , 19 , 33 , 69 , 70 , 72 , 73 ]. Introgression of exotic genes into elite germplasm might be accompanied with the transfer of undesired morphological trait, thus mining and stacking minor-effect resistance QTL from adapted durum germplasm will contribute to enrich the gene pool and improve resistance levels without compromising the elite germplasm characteristics.…”

Section: Discussionmentioning

confidence: 99%

Genetic mapping of deoxynivalenol and fusarium damaged kernel resistance in an adapted durum wheat population

Berraies,

Ruan,

Knox

et al. 2024

BMC Plant Biol

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Background Fusarium head blight (FHB) infection results in Fusarium damaged kernels (FDK) and deoxynivalenol (DON) contamination that are downgrading factors at the Canadian elevators. Durum wheat (Triticum turgidum L. var. durum Desf.) is particularly susceptible to FHB and most of the adapted Canadian durum wheat cultivars are susceptible to moderately susceptible to this disease. However, the durum line DT696 is less susceptible to FHB than commercially grown cultivars. Little is known about genetic variation for durum wheat ability to resist FDK infection and DON accumulation. This study was undertaken to map genetic loci conferring resistance to DON and FDK resistance using a SNP high-density genetic map of a DT707/DT696 DH population and to identify SNP markers useful in marker-assisted breeding. One hundred twenty lines were grown in corn spawn inoculated nurseries near Morden, MB in 2015, 2016 and 2017 and the harvested seeds were evaluated for DON. The genetic map of the population was used in quantitative trait locus analysis performed with MapQTL.6® software. Results Four DON accumulation resistance QTL detected in two of the three years were identified on chromosomes 1 A, 5 A (2 loci) and 7 A and two FDK resistance QTL were identified on chromosomes 5 and 7 A in single environments. Although not declared significant due to marginal LOD values, the QTL for FDK on the 5 and 7 A were showing in other years suggesting their effects were real. DT696 contributed the favourable alleles for low DON and FDK on all the chromosomes. Although no resistance loci contributed by DT707, transgressive segregant lines were identified resulting in greater resistance than DT696. Breeder-friendly KASP markers were developed for two of the DON and FDK QTL detected on chromosomes 5 and 7 A. Markers flanking each QTL were physically mapped against the durum wheat reference sequence and candidate genes which might be involved in FDK and DON resistance were identified within the QTL intervals. Conclusions The DH lines harboring the desired resistance QTL will serve as useful resources in breeding for FDK and DON resistance in durum wheat. Furthermore, breeder-friendly KASP markers developed during this study will be useful for the selection of durum wheat varieties with low FDK and DON levels in durum wheat breeding programs.

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Section: Discussionmentioning

confidence: 99%

Genetic mapping of deoxynivalenol and fusarium damaged kernel resistance in an adapted durum wheat population

Berraies,

Ruan,

Knox

et al. 2024

BMC Plant Biol

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Untargeted metabolomics approaches for the characterization of cereals and their derived products by means of liquid chromatography coupled to high resolution mass spectrometry

Bozza,

Barboni,

Spadafora

et al. 2024

Journal of Chromatography Open

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Coping with salinity stress: segmental group 7 chromosome introgressions from halophytic Thinopyrum species greatly enhance tolerance of recipient durum wheat

Tounsi,

Giorgi,

Kuzmanović

et al. 2024

Front. Plant Sci.

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Increased soil salinization, tightly related to global warming and drought and exacerbated by intensified irrigation supply, implies highly detrimental effects on staple food crops such as wheat. The situation is particularly alarming for durum wheat (DW), better adapted to arid/semi-arid environments yet more sensitive to salt stress than bread wheat (BW). To enhance DW salinity tolerance, we resorted to chromosomally engineered materials with introgressions from allied halophytic Thinopyrum species. “Primary” recombinant lines (RLs), having portions of their 7AL arms distally replaced by 7el1L Th. ponticum segments, and “secondary” RLs, harboring Th. elongatum 7EL insertions “nested” into 7el1L segments, in addition to near-isogenic lines lacking any alien segment (CLs), cv. Om Rabia (OR) as salt tolerant control, and BW introgression lines with either most of 7el1 or the complete 7E chromosome substitution as additional CLs, were subjected to moderate (100 mM) and intense (200 mM) salt (NaCl) stress at early growth stages. The applied stress altered cell cycle progression, determining a general increase of cells in G1 and a reduction in S phase. Assessment of morpho-physiological and biochemical traits overall showed that the presence of Thinopyrum spp. segments was associated with considerably increased salinity tolerance versus its absence. For relative water content, Na+ accumulation and K+ retention in roots and leaves, oxidative stress indicators (malondialdehyde and hydrogen peroxide) and antioxidant enzyme activities, the observed differences between stressed and unstressed RLs versus CLs was of similar magnitude in “primary” and “secondary” types, suggesting that tolerance factors might reside in defined 7el1L shared portion(s). Nonetheless, the incremental contribution of 7EL segments emerged in various instances, greatly mitigating the effects of salt stress on root and leaf growth and on the quantity of photosynthetic pigments, boosting accumulation of compatible solutes and minimizing the decrease of a powerful antioxidant like ascorbate. The seemingly synergistic effect of 7el1L + 7EL segments/genes made “secondary” RLs able to often exceed cv. OR and equal or better perform than BW lines. Thus, transfer of a suite of genes from halophytic germplasm by use of fine chromosome engineering strategies may well be the way forward to enhance salinity tolerance of glycophytes, even the sensitive DW.

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Untargeted Metabolomics Reveals a Multi-Faceted Resistance Response to Fusarium Head Blight Mediated by the Thinopyrum elongatum Fhb7E Locus Transferred via Chromosome Engineering into Wheat

Cited by 3 publications

References 142 publications

Genetic mapping of deoxynivalenol and fusarium damaged kernel resistance in an adapted durum wheat population

Genetic mapping of deoxynivalenol and fusarium damaged kernel resistance in an adapted durum wheat population

Untargeted metabolomics approaches for the characterization of cereals and their derived products by means of liquid chromatography coupled to high resolution mass spectrometry

Coping with salinity stress: segmental group 7 chromosome introgressions from halophytic Thinopyrum species greatly enhance tolerance of recipient durum wheat

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