2015
DOI: 10.1016/j.soilbio.2014.10.007
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Untargeted soil metabolomics methods for analysis of extractable organic matter

Abstract: a b s t r a c tThe cycling of soil organic matter (SOM) by microorganisms is a critical component of the global carbon cycle but remains poorly understood. There is an emerging view that much of SOM, and especially the dissolved fraction (DOM), is composed of small molecules of plant and microbial origin resulting from lysed cells and released metabolites. Unfortunately, little is known about the small molecule composition of soils and how these molecules are cycled (by microbes or plants or by adsorption to m… Show more

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Cited by 162 publications
(132 citation statements)
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References 60 publications
(80 reference statements)
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“…We investigated the distribution of metabolites in triplicate extracted soil water samples using a robust Liquid Chromatography-based Mass Spectroscopy (LC-MS) metabolomics workflow based on a previous study on samples from the meadow soils (Swenson et al, 2015). A total of 125 unique compounds were detected and quantified.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…We investigated the distribution of metabolites in triplicate extracted soil water samples using a robust Liquid Chromatography-based Mass Spectroscopy (LC-MS) metabolomics workflow based on a previous study on samples from the meadow soils (Swenson et al, 2015). A total of 125 unique compounds were detected and quantified.…”
Section: Resultsmentioning
confidence: 99%
“…Metabolites were extracted from sieved (2 mm) soil samples using an aqueous extraction protocol (modified from Swenson et al, 2015). Briefly, sieved soils (4 g) were incubated (200 rpm, 1 h, 4°C) in triplicate with MilliQ water (16 ml) amended with 1.6 µg/ml of ABMBA (2-Amino-3-Bromo-5-methylbenzoic acid) and 2 µg/ml of UL-13C-Glucose included as internal extraction standards.…”
Section: Methodsmentioning
confidence: 99%
“…organic and amino acids, coumarins) that do not address the biochemical diversity of rhizosphere soil. Recent advances in liquid chromatography, mass spectrometry, and uni-and multivariate data analysis have made it possible to conduct untargeted metabolic profiling of complex metabolite mixtures, such as root exudates and soil extracts (Khorassani et al, 2011;Strehmel et al, 2014;Swenson et al, 2015;Ziegler et al, 2015;van Dam and Bouwmeester, 2016). In this study, we employed untargeted UPLC-Q-TOF analysis of soil extracts, followed by uni-and multivariate data reduction to separate rhizosphere-specific chemistry from common soil chemistry.…”
Section: Discussionmentioning
confidence: 99%
“…Specialized imaging techniques, such as magnetic resonance imaging, computed tomography (Metzner et al ., ; Helliwell et al ., ), or the use of labeled plants (Rellán‐Álvarez et al ., ), have been developed, but they are not widely accessible or amenable to high‐throughput experimentation (Metzner et al ., ). Similarly, approaches for the investigation of root exudation in soils include the use of in situ soil drainage systems (lysimeters) in fields (Strobel, ), which are low throughput and require complex installations, or of laboratory‐based extraction methods that are based on flushing the soil with large volumes of liquids (Swenson et al ., ; Pétriacq et al ., ). Studying metabolites within rhizosphere soils is also challenging because of the complex mixture of plant‐ and microbe‐derived metabolites, which are potentially altered by the chemistry and mineralogy of the soil investigated.…”
Section: Introductionmentioning
confidence: 99%