Unterscheidung der Penicilloinsäuren von funktionellen Derivaten ihrer. α‐Carboxylgruppe mittels Penamaldat‐Stabilitätsbestimmung

Schneider, Conrad H.; Weck, A.L. de

doi:10.1002/hlca.19660490531

Cited by 35 publications

(14 citation statements)

References 5 publications

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“…The reaction of mercuric chloride with the sulfhydryl groups [Schultze and Heremans, 1966] on y-globulins may be responsible for this phenomenon. The penamaldate assay has been widely used to determine the mo lar ratio of BPO groups to carriers [Parker et al, 1962;Schneider et al, 1966Schneider et al, , 1971. Particular attention should be paid to prote in concentration on performance of the pen amaldate assay, when y-globulins are em ployed as carrier proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Fully substituted benzylpenicil loyl poly-L-Iysine (BPO-PLL) was prepared as de scribed previously [Schneider et al, 1971]. The molar ratio of BPO groups to the carrier was de termined by the penamaldate assay [Schneider and de Week, 1966]. In brief, each conjugate was ap propriately diluted with 0.05 M phosphate-buf fered saline (PBS), pH 7.4, and a 1-ml sample was mixed with 0.04 ml of 1 mM mercuric chloride.…”

Section: Preparation Of Hapten-carrier Conjugatesmentioning

confidence: 99%

“…The BPO hapten is used because it is one of the few chemically well-defined allergens, which plays an important role in human allergic disorders [Parker et al, 1962;Blaser et al, 1980]. The penamaldate assay, which has been used for quantitative titration for the molar ratio of BPO groups to the carrier molecules [Parker et al, 1962;Schneider and de Week, 1966] was also reevaluated.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Epitoge Density on the Induction of the IgE Immune Response in Mice

Nakagawa¹,

Blaser²,

Weck³

1980

Int Arch Allergy Immunol

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The effect of hapten density on benzylpenicilloyl (BPO)-protein conjugates upon the induction of BPO-specific IgE immune response was investigated in BALB/c and C3H mice. Bovine γ-globulin (BGG) and ovalbumin (OA) were employed as carrier proteins. In both strains of mice, moderately substituted conjugates (BPO₁₉-BGG, and BPO-OA with an epitope density from 2 to 7.5) elicited a persistent BPO-specific IgE, while heavily substituted conjugates (BPO₃₈-BGG) induced only a transient response. In contrast, lightly substituted conjugates (BPO_3.2-, BPO_9.5-BGG, and BPO₁-OA) failed to produce BPO-specific IgE antibodies. There was no significant difference in carrier-specific IgE immune response among these various conjugates. These results suggest that moderately substituted hapten-protein conjugates are one of the favorable conditions for eliciting hapten-specific IgE immune response in mice.

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Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Hapten-carrier Conjugatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Epitoge Density on the Induction of the IgE Immune Response in Mice

Nakagawa¹,

Blaser²,

Weck³

1980

Int Arch Allergy Immunol

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“…This is also true for the data of the elemental analyses and the molar penamaldate values ( Table 2). The molar penamaldate value of conjugate 4 (disregarding the monohaptenic compound 1) is nevertheless among the two highest ones, and it is expected that marked incompleteness of penicilloylation will be [7] divided by the theoretical number of Bpo-groups. It is of the order of 16000-18000 for disubstituted and multisubstituted Bpo-derivatives (cf: [El).…”

mentioning

confidence: 99%

Preparation and Conformational Properties of Benzylpenicilloyl‐oligo‐L‐lysine Conjugates

Rolli

Lüscher

Schneider

et al. 1982

Helvetica Chimica Acta

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SummarySelected oligo-L-lysine carriers and a poly-L-lysine were penicilloylated with benzylpenicillin. The resulting conjugates 2-6 were studied by IR. absorption in the solid state and circular dichroism measurements in solution. The IR. data demonstrate the lack of /3-structure formation even in medium-sized peptides where such structures might be expected on the basis of previous studies on differen tly substituted oligo-L-lysines. Considerable proportions of right-handed a-helical conformation are exhibited by the icosa-L-lysine and poly-L-lysine conjugates 5 and 6 in water and 2,2,2-trifluoroethanol. Difficulties in obtaining fully penicilloylated conjugates are not related to the extent of a-helical conformation in aqueous solution.

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“…After passage through a Sephadex G-25 column (pH 5.6), the BPO-DEX eluate was immediately frozen and stored at -20 °C until use. A substitution of approximately 60 BPO groups per 100 glucose residues was measured by the penamaldate assay [9] and by the anthron meth od [10]. Hydrolysis of BPO-DEX was also con firmed as described [5].…”

Section: Chemicalsmentioning

confidence: 99%

Induction of Immunological Tolerance to the Penicilloyl Antigenic Determinant

Nakagawa¹,

Otz²,

Weck³

et al. 1981

Int Arch Allergy Immunol

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The suppressive effect of cleavable penicilloylated dextran (BPO-DEX), whose directly bound penicilloyl groups undergo hydrolytic cleavage within 3 days under physiological conditions, on murine IgE antibody formation against the benzylpenicilloyl (BPO) determinant was investigated in BALB/c and C3H mice. Intraperitoneal administration of BPO-DEX during either primary or secondary IgE responses to BPO ascaris produced a reversible suppression of BPO-specific IgE, while not affecting carrier-specific IgE antibody formation. Suppression of longer duration, at least 10 weeks, was achieved, however, by repeated administrations of BPO-DEX. BPO-DEX itself did not generate detectable BPO-specific IgE antibodies. These results suggest that BPO-DEX might be one of the promising tolerogens in the prevention of penicillin allergy.

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Unterscheidung der Penicilloinsäuren von funktionellen Derivaten ihrer. α‐Carboxylgruppe mittels Penamaldat‐Stabilitätsbestimmung

Cited by 35 publications

References 5 publications

Effect of Epitoge Density on the Induction of the IgE Immune Response in Mice

Effect of Epitoge Density on the Induction of the IgE Immune Response in Mice

Preparation and Conformational Properties of Benzylpenicilloyl‐oligo‐L‐lysine Conjugates

Induction of Immunological Tolerance to the Penicilloyl Antigenic Determinant

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