Untersuchung zur quantitativen Auswertung der Dünnschichtchromatographie I UV‐spektroskopische Bestimmung von p‐Hydroxybenzoesäuremethylester‐ und p‐Hydroxybenzoesäurepropylester‐Gemischen nach dünnschichtchromatographischer Trennung

Gänshirt, H.; Morianz, K.

doi:10.1002/ardp.19602931207

Cited by 56 publications

(10 citation statements)

References 2 publications

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“…priming dose delivered from an Agla micrometer syringe (Burroughs Wellcome). Oleic [Ganshirt et al, 1960]. Taurocholate predominated, but other conjugated and unconjugated bile acids weie present.…”

Section: Methodsmentioning

confidence: 80%

The Effect of Bile Salts on the Lymphatic Absorption by the Unanæsthetized Rat of Intraduodenally Infused Lipids

Morgan

1964

Exp Physiol

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Emulsified and unemulsified fat was infused intraduodenally at a steady rate in unanaesthetized rats with lymph fistulhe. The output of esterified fat in the lymph reached a steady state which was considered to mirror fat absorption from the lumen. In animals which also had bile fistulae the effect of addition or removal of sodium taurocholate was measured.Unemulsified olive oil or oleic acid and mono-olein sol was poorly absorbed in the absence of bile. Emulsified olive oil was better, but still incompletely, absorbed. In all these cases the addition of sodium taurocholate increased the absorption to that found in control animals with normal bile flow. Emulsified oleic acid was as well absorbed in bile fistula animals as in controls and the return or removal of taurocholate did not affect the output of esterified fat in the lymph. These results do not support a defect in epithelial re-esterification of fatty acids in the absence of bile.WHEN fat is infused at a steady rate directly into the duodenum of unanawsthetized rats, within a few hours the output of fat in the thoracic duct lymph reaches a plateau mirroring the absorption of fat [Shepherd and Simmonds, 1959]. When fat absorption is altered after a steady state has been established, the change is soon reflected in the lymphatic output of fat [Bennett et al., 1962] and so each experiment provides its own control period. This technique has been used to determine the effect of bile salts on the absorption of emulsified and unemulsified olive oil and oleic acid and of a mono-olein sol in rats with external bile fistulae. The aim was to evaluate the relative importance of the effect on lipolysis and on dispersion of triglyceride and digestion products in the lumen, on re-esterification during passage through the epithelium [Dawson and Isselbacher, 1960] and on the pathway of absorption [Borgstrom, 1953]. METHODSOperative Procedures-Male albino rats weighing 200-220 g. were fasted overnight. Under ether anaesthesia a thoracic duct fistula was established in each rat [Bollman et al., 1948] and at the same time a fine silicone rubber cannula was introduced into the duodenum through a fundal incision in the stomach. The tip of the duodenal * This work was carried out with the aid of grants from the National Health an(d Medical Research Council, Canberra, and the Medical Researchl Grants Committee, The

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Section: Methodsmentioning

confidence: 80%

The Effect of Bile Salts on the Lymphatic Absorption by the Unanæsthetized Rat of Intraduodenally Infused Lipids

Morgan

1964

Exp Physiol

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“…Bile salts were separated by t.l.c. on silica-gel H plates by an adaptation of the method of Ganshirt et al (1960). Bile (1 vol.)…”

Section: Methodsmentioning

confidence: 99%

Membranes and bile formation. Composition of several mammalian biles and their membrane-damaging properties

Coleman¹,

Iqbal²,

Godfrey³

et al. 1979

Biochemical Journal

212

117

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The total content and profile of bile salts and phospholipids are reported for several mammalian biles. Rabbit and guinea-pig biles are characterized by high proportions of conjugated dihydroxy bile salts with respect to trihydroxy bile salts, but contain relatively little phospholipid. Both rabbit and guinea-pig biles exhibit little evidence of hepatic cell damage, even though they are able to cause membrane damage (as evidenced by lysis of human erythrocytes) at low (2--3 mM) concentrations of bile salts; this lytic behaviour is also a property of their predominant bile salts. Addition of phosphatidylcholine to the bile or bile salt is able to decrease the lytic behaviour. Perhaps the most significant observation is that these biles, and their predominant bile salts, are dramatically less lytic towards sheep erythrocytes, indicating that some factor(s) in membrane composition and structure may partly explain the resistance of membranes of the biliary tract to the presence of high concentrations of potentially membrane-damaging bile salts.

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“…in a butan-1-ol/acetic acid/water (10 :1 :1, by vol.) system (Ganshirt et al, 1960) showed that at least 95% of the 14C radioactivity, identified by com-parison with authentic samples, was present as taurodehydrocholate.…”

Section: Preparation Of [14c]taurodehydrocholatementioning

confidence: 98%

The effects of colchicine on secretion into bile of bile salts, phospholipids, cholesterol and plasma membrane enzymes: bile salts are secreted unaccompanied by phospholipids and cholesterol

Barnwell¹,

Lowe²,

Coleman³

1984

Biochemical Journal

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Colchicine, a drug which interferes with microtubular function, has no effect on the secretion of taurodehydrocholate into bile; it is therefore suggested that bile salts are unlikely to be packaged in vesicles during cellular transit from sinusoidal to canalicular membranes. Colchicine greatly reduces the secretion of phospholipid and cholesterol into bile; it is suggested that this is due to an interruption in the supply of vesicles bringing lipids to repair the canalicular membrane during bile salt output. In the absence of the protective effect of a continuous supply of repair vesicles, micelleforming bile salts damage the canalicular membrane; the increased concentration of plasma membrane enzymes in bile and the increased aspartate aminotransferase activity in plasma and bile are evidence of this damage. Damage to the canalicular membrane may also be an explanation for the reduction in taurocholate transport and the taurocholate-induced cholestasis which are seen with colchicine-treated livers. Such membrane damage is not observed in colchicine-treated livers during the secretion of the non-micelle forming bile salt, taurodehydrocholate.

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Untersuchung zur quantitativen Auswertung der Dünnschichtchromatographie I UV‐spektroskopische Bestimmung von p‐Hydroxybenzoesäuremethylester‐ und p‐Hydroxybenzoesäurepropylester‐Gemischen nach dünnschichtchromatographischer Trennung

Cited by 56 publications

References 2 publications

The Effect of Bile Salts on the Lymphatic Absorption by the Unanæsthetized Rat of Intraduodenally Infused Lipids

The Effect of Bile Salts on the Lymphatic Absorption by the Unanæsthetized Rat of Intraduodenally Infused Lipids

Membranes and bile formation. Composition of several mammalian biles and their membrane-damaging properties

The effects of colchicine on secretion into bile of bile salts, phospholipids, cholesterol and plasma membrane enzymes: bile salts are secreted unaccompanied by phospholipids and cholesterol

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